Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,664,399	A→G	L74L (TTA→CTA)	gadW ←	DNA‑binding transcriptional dual regulator GadW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,664,399	0	A	G	100.0%	128.2 / NA	38	L74L (TTA→CTA)	gadW	DNA‑binding transcriptional dual regulator GadW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/24);  total (14/24)

ATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATAC  >  NC_000913/3664267‑3664533                                                                                                                                     |                                                                                                                                       aTAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTaaa                                                                                                                                 <  2:157645/140‑1 (MQ=255)       aGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAAATAACAAATGGTATTTGCTGTGCAGCCCTAAATTGCGt                                                                                                                           >  1:261670/1‑140 (MQ=255)              gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCt                                                                                                                    <  2:371112/140‑1 (MQ=255)              gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCt                                                                                                                    <  2:399194/140‑1 (MQ=255)                agagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGa                                                                                                                  <  1:144924/140‑1 (MQ=255)                agagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGa                                                                                                                  <  1:13843/140‑1 (MQ=255)                   gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGa                                                                                                               >  1:2206/1‑140 (MQ=255)                     atatTGCTCAGTATCCGTGAATTTACCCCTGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAat                                                                                                             <  1:195491/140‑1 (MQ=255)                        ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGGTGTGTAGGCCTGAAATGCGTGTTAGCTGACGAATAtt                                                                                                          >  1:193235/1‑140 (MQ=255)                                tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaagt                                                                                                  >  2:504382/1‑140 (MQ=255)                                   cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTATTTGCTGTGTAGCCCGAAATTGCGTGGCTGATGACGAGTATTTTGTAAGTCAt                                                                                               >  1:234196/1‑140 (MQ=255)                                     gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAat                                                                                                             >  2:719021/1‑124 (MQ=255)                                     gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAat                                                                                                             <  1:719021/124‑1 (MQ=255)                                      tGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                                                                            <  2:176815/140‑1 (MQ=255)                                             aCCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                                                                     <  1:504382/140‑1 (MQ=255)                                             aCCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                                                                     <  2:559111/140‑1 (MQ=255)                                                   gATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                                                                >  2:176189/1‑139 (MQ=255)                                                   gATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                                                                <  1:176189/139‑1 (MQ=255)                                                             aGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATCTTTGGTAGGTAATGGCCAACTGTCAGCTCATCGATATcc                                                                     >  2:268508/1‑140 (MQ=255)                                                             aGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATcc                                                                     >  2:613667/1‑140 (MQ=255)                                                                tATCAAAAATCGCCGTCACCAGAGGGATGTGGGGTGATTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                                                                  <  1:281714/140‑1 (MQ=255)                                                                 aTCAAAAATCGCCGTCAACAACAGCATGGGGAGTGACTGATTAATTAAAAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtc                                                                 <  2:244268/140‑1 (MQ=255)                                                                   cAAAAATCGCCGTCACCAGCGGAATCGGGGGTGACTGCTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtcgt                                                               <  1:268508/140‑1 (MQ=255)                                                                      aaaTCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAGCTGTCAGCTCATCGATATCCAGTCGTcgc                                                            <  2:662864/140‑1 (MQ=255)                                                                          cGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGACAAACTGGCAGATCATCGATAGCCAGTCGTCGCGTAt                                                        >  1:204547/1‑140 (MQ=255)                                                                                    aGCGGCATGGGGGGTGTTTGGTAAATTAACAAACGATCTTTGCTGTGTCGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACa                                              <  2:322913/140‑1 (MQ=255)                                                                                             gggggTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCaaa                                     >  2:658171/1‑140 (MQ=255)                                                                                                     cTGGTTAATTAACAAACGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTg                             >  2:160747/1‑140 (MQ=255)                                                                                                             ttaaCAAATGGTCTTTATTGTGAAGCCCTAAATTGAGTGGTAGCTGACTAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAAttt                     <  2:356263/140‑1 (MQ=255)                                                                                                                   aaCGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTcc               >  2:85253/1‑140 (MQ=255)                                                                                                                     cGGTCTTTGCTGTTTAGTCCTAAATTGCGTGGTAGATGATGAATATTTCGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAAACGTCTGCCAAATTTTTCTCcag             <  2:234196/140‑1 (MQ=255)                                                                                                                     cGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCcag             <  1:376440/140‑1 (MQ=255)                                                                                                                     cGGTCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCcag             <  1:160747/140‑1 (MQ=255)                                                                                                                        tCTTTGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCcagcag          <  1:613667/140‑1 (MQ=255)                                                                                                                          tttGCTGTGTAGCCCTACATTGTGTGGTAGCTGACCAATCTTTTGTAATTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCcagcagca        <  2:193235/140‑1 (MQ=255)                                                                                                                          tttGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAATTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCcagcagca        <  2:294317/140‑1 (MQ=255)                                                                                                                          tttGCTGTGTAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCcagcagca        >  1:625123/1‑140 (MQ=255)                                                                                                                                gtgtAGCCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATAc  <  1:658171/140‑1 (MQ=255)                                                                                                                                gtgtAGCCCTAAATTGCATGGTCGCTGACGATTATGTTGCAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATAc  <  2:204547/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       ATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATAC  >  NC_000913/3664267‑3664533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑