Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,740,385	T→C	G227G (GGT→GGC)	avtA →	valine‑‑pyruvate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,740,385	0	T	C	100.0%	109.5 / NA	32	G227G (GGT→GGC)	avtA	valine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGAAAAAATCATCACCGCCAT  >  NC_000913/3740262‑3740513                                                                                                                            |                                                                                                                                 ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACACTTATGGGGTCCCGTTCCCGGCCATCATCTTCAGTGAAg                                                                                                                  >  2:272536/1‑140 (MQ=255)    gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAgcgc                                                                                                               <  1:551120/140‑1 (MQ=255)     ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGG‑GAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAgcgcgc                                                                                                             <  2:163532/140‑1 (MQ=255)       gacgaATCCAACAGGGAATGTGATTACTGACGAAGAGCTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGcc                                                                                                            <  1:272536/140‑1 (MQ=255)               cAACAGGCAATGTGATTTCTGAAGAAGAGTTCCTGTAGCCTGACGCGCCGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATgg                                                                                                    <  2:186136/140‑1 (MQ=255)               cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATgg                                                                                                    <  1:567536/140‑1 (MQ=255)                      cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGa                                                                                             >  1:393866/1‑140 (MQ=255)                      cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGa                                                                                             <  1:398426/140‑1 (MQ=255)                       aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGaa                                                                                            >  1:14203/1‑140 (MQ=255)                              ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTg                                                                                     <  2:153651/140‑1 (MQ=255)                              ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTg                                                                                     <  1:371127/140‑1 (MQ=255)                              ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTg                                                                                     <  1:675284/140‑1 (MQ=255)                                    aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGGATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGc                                                                               >  1:651921/1‑140 (MQ=255)                                    aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGc                                                                               >  1:584998/1‑140 (MQ=255)                                       aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATg                                                                            >  2:74656/1‑140 (MQ=255)                                       aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATg                                                                            >  2:733421/1‑140 (MQ=255)                                          agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGt                                                                         <  1:74656/140‑1 (MQ=255)                                              gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCTAAGATCGTGATGTGCATGAGTCttt                                                                     >  1:343896/1‑140 (MQ=255)                                              gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAgctgtc                                                                     >  1:728628/1‑135 (MQ=255)                                              gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCttt                                                                     >  1:685400/1‑140 (MQ=255)                                                   aGCTTGACGCGCTGGCGAATCACCAGGGCCTTCCGCTTGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAg                                                                <  1:246340/140‑1 (MQ=255)                                                    gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGc                                                               <  1:733421/140‑1 (MQ=255)                                                    gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGc                                                               >  2:40014/1‑140 (MQ=255)                                                    gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGc                                                               <  1:40014/140‑1 (MQ=255)                                                                         acacGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCt                                          <  2:651921/140‑1 (MQ=255)                                                                                       tGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATAAGTCTTTCGAAGGTTGTTCTACCTGGCTCCTACTGCGGCATTatcatc                            >  2:360879/1‑140 (MQ=255)                                                                                                atAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGaa                   <  1:512670/140‑1 (MQ=255)                                                                                                   aCGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGaaaaa                >  2:486729/1‑140 (MQ=255)                                                                                                          tGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGAAAAAAtcatca         >  2:604163/1‑140 (MQ=255)                                                                                                                 ccGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCCCCCGCTGCGGCATTATCATCGCCAATGAAAAAATCATCACCGCCAt  <  1:471262/140‑1 (MQ=255)                                                                                                                 ccGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGCCTCCCGCTACGGGATTATCATCGCCGATGAAAAAATCATCACCGCCAt  >  2:310824/1‑140 (MQ=255)                                                                                                                     tCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGAAAAAATCATCACCGcc    <  1:591417/134‑1 (MQ=255)                                                                                                                     tCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGAAAAAATCATCACCGcc    >  2:591417/1‑134 (MQ=255)                                                                                                                            |                                                                                                                                 CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGAAAAAATCATCACCGCCAT  >  NC_000913/3740262‑3740513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑