Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,750,873	A→G	intergenic (+92/+255)	sgbE → / ← yiaT	L‑ribulose‑5‑phosphate 4‑epimerase SgbE/outer membrane protein YiaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,750,873	0	A	G	100.0%	189.9 / NA	56	intergenic (+92/+255)	sgbE/yiaT	L‑ribulose‑5‑phosphate 4‑epimerase SgbE/outer membrane protein YiaT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/24);  total (32/24)

ACCTGCGTAAGCATGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTATGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACATTTGAAGGCAAG  >  NC_000913/3750741‑3751004                                                                                                                                     |                                                                                                                                    aCCTGCGTAAGCATGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGTCGCTTCCCCGCTGCAGGCGTTAAACCAGTGTGTTTCt                                                                                                                              >  1:249100/1‑140 (MQ=255)  ccTGCGTAAGCATGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTg                                                                                                                             >  1:723057/1‑140 (MQ=255)     gCGTAAGCATGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCGTCCACGCGGCTGGCGTTAAACAAGTGTGTTTCTGaaa                                                                                                                          >  1:195117/1‑140 (MQ=255)      cGTAAGCATGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGaaaa                                                                                                                         >  2:626093/1‑140 (MQ=255)          aGCATGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCt                                                                                                                     >  1:455989/1‑140 (MQ=255)            cATGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGc                                                                                                                   >  2:625151/1‑140 (MQ=255)             aTGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGcc                                                                                                                  <  1:120513/140‑1 (MQ=255)                     aaTGCCTATTACGGGCAGTAATCCCCCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAg                                                                                                          <  1:285338/140‑1 (MQ=255)                       tGCCTATTACGGGCAGTCATCCTTCACGCCGCGGCCTCAACGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGcc                                                                                                        <  2:249100/140‑1 (MQ=255)                       tGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGTTGCTGGCGCTAAACCATTGTGTTTCAGAAAGATCTGCCGGAAATAGcc                                                                                                        >  2:285338/1‑140 (MQ=255)                           tATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACg                                                                                                    >  2:539877/1‑140 (MQ=255)                            aTTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGt                                                                                                   >  2:118924/1‑140 (MQ=255)                              tACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCa                                                                                                 <  2:431768/140‑1 (MQ=255)                                   gCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTaaa                                                                                            >  2:81679/1‑140 (MQ=255)                                   gCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTaaa                                                                                            >  2:149071/1‑140 (MQ=255)                                   gCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTaaa                                                                                            <  1:81679/140‑1 (MQ=255)                                   gCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTaaa                                                                                            <  1:47059/140‑1 (MQ=255)                                   gCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTaaa                                                                                            <  1:118924/140‑1 (MQ=255)                                     aGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCaa                                                                                                      >  1:660461/1‑128 (MQ=255)                                     aGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCaa                                                                                                      <  2:660461/128‑1 (MQ=255)                                       tAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCTGTGTGTTTCTGAAAAAACTGCCGGAAATAGATAACGTCATTAAACCGt                                                                                        >  2:321676/1‑140 (MQ=255)                                       tAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATTTGCCGGAAATAGCCAACGTCATTAAACCGt                                                                                        >  1:2870/1‑140 (MQ=255)                                       tAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGt                                                                                        >  2:154894/1‑140 (MQ=255)                                       tAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGt                                                                                        >  1:644301/1‑140 (MQ=255)                                       tAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGt                                                                                        >  1:412415/1‑140 (MQ=255)                                        aaTCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGGTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTa                                                                                       <  1:539877/140‑1 (MQ=255)                                         aTCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTAt                                                                                      >  2:657419/1‑140 (MQ=255)                                             cTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCa                                                                                  <  2:412415/140‑1 (MQ=255)                                               cACGCCGGGGCATCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATg                                                                                >  1:590229/1‑140 (MQ=255)                                                 cGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGCGTGTTTCTGAAAAATCTGCTGGAAATAGCCAACGTCATTAAACCGTATTGCATGGa                                                                              >  2:67243/1‑140 (MQ=255)                                                    cGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCa                                                                           >  2:510641/1‑140 (MQ=255)                                                          ttCATCGCCCCGGCAGTACGAATTGGTATTTTCCTTGGTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGa                                                                     <  1:321676/140‑1 (MQ=255)                                                           tCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGAc                                                                    <  1:626093/140‑1 (MQ=255)                                                              tCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAAc                                                                 >  1:123772/1‑140 (MQ=255)                                                                  cccGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCg                                                             <  1:596882/140‑1 (MQ=255)                                                                     ggCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGt                                                          <  1:154894/140‑1 (MQ=255)                                                                     ggCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGt                                                          <  1:176377/140‑1 (MQ=255)                                                                            cGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCa                                                   <  2:723057/140‑1 (MQ=255)                                                                            cGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGACATTAAACGGTATTGCTTGGAGCAGGCCGAGAACGTCGAGTCACTGCa                                                   >  2:321135/1‑140 (MQ=255)                                                                               aTTGATATGTTCCTTGCTGTAACGCGGCTGCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATaa                                                <  1:321135/140‑1 (MQ=255)                                                                                    tatGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGCGTGTTTCTGAAAAATCTGCTGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGt                                           <  1:67243/140‑1 (MQ=255)                                                                                        ttCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTtgct                                       >  1:670444/1‑140 (MQ=255)                                                                                             tGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGCGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCGGACAACGTCGAGTCACTGCATTACCTGTTGCTGCccg                                  >  1:240099/1‑140 (MQ=255)                                                                                                 gTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGa                              <  2:2870/140‑1 (MQ=255)                                                                                                  tAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGAt                             >  1:422147/1‑140 (MQ=255)                                                                                                   aaCGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATg                            <  2:670444/140‑1 (MQ=255)                                                                                                    aCGCCGCTTCCACGCTGCTGGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATgc                           >  2:398411/1‑140 (MQ=255)                                                                                                     cgccgcTTCCACGCTGCGGGCGGTAAACCAGTGTGTTTCAGAAAAATCGGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATgcg                          <  2:240099/140‑1 (MQ=255)                                                                                                                    gctgGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACAttt           >  1:112853/1‑140 (MQ=255)                                                                                                                     ctgGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACATTTg          >  2:378644/1‑140 (MQ=255)                                                                                                                     ctgGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACATTTg          >  2:603898/1‑140 (MQ=255)                                                                                                                     ctgGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGGCACTGCATAACCGGTTGCTGCCCGCGGATGAGCCATTTACGACATTTg          >  2:203535/1‑140 (MQ=255)                                                                                                                      tgGCGTTAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACATTTGa         <  2:123772/140‑1 (MQ=255)                                                                                                                           ttAACCCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACATTTGAAGGCa    <  1:58716/140‑1 (MQ=255)                                                                                                                           ttAAACCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACATTTGAAGGCa    <  2:461822/140‑1 (MQ=255)                                                                                                                             aaaCCAGTGTGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACATTTGAAGGCAAg  >  2:178624/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                    ACCTGCGTAAGCATGGGGCCAATGCCTATTACGGGCAGTAATCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGCGTTAAACCAGTATGTTTCTGAAAAATCTGCCGGAAATAGCCAACGTCATTAAACCGTATTGCATGGAGCAGGCCGACAACGTCGAGTCACTGCATAACCTGTTGCTGCCCGCCGATGCGCCATTTACGACATTTGAAGGCAAG  >  NC_000913/3750741‑3751004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑