Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,753,644	G→A	S152S (AGC→AGT)	yiaV ←	putative membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,753,644	0	G	A	100.0%	176.6 / NA	51	S152S (AGC→AGT)	yiaV	putative membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/27);  total (24/27)

GCTATCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGGCTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCGCTGTTTATGTTCTGCGGTAACGATATCC  >  NC_000913/3753518‑3753776                                                                                                                               |                                                                                                                                     gCTATCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGt                                                                                                                         <  2:92746/140‑1 (MQ=255) gCTATCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGt                                                                                                                         <  1:385365/140‑1 (MQ=255) gCTATCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGt                                                                                                                         >  1:92746/1‑140 (MQ=255)   tATCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTaa                                                                                                                       <  2:555444/140‑1 (MQ=255)   tATCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTaa                                                                                                                       <  1:402557/140‑1 (MQ=255)    aTCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAAc                                                                                                                      >  2:447135/1‑140 (MQ=255)     tCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCAc                                                                                                                               <  1:15208/130‑1 (MQ=255)     tCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCAc                                                                                                                               >  2:15208/1‑130 (MQ=255)     tCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACg                                                                                                                     >  2:31037/1‑140 (MQ=255)     tCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACg                                                                                                                     >  1:720903/1‑140 (MQ=255)          cTGGCTCTGGATCTGATTTTGTTCCTCCGACGATGACGTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGAt                                                                                                                <  1:320191/140‑1 (MQ=255)             gCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATAct                                                                                                             >  2:93131/1‑140 (MQ=255)               tctGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATActct                                                                                                           >  2:484850/1‑140 (MQ=255)               tctGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATActct                                                                                                           <  1:484850/140‑1 (MQ=255)                  ggATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTa                                                                                                        >  2:451404/1‑140 (MQ=255)                   gATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAg                                                                                                       <  1:524894/140‑1 (MQ=255)                   gATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAg                                                                                                       <  1:393867/140‑1 (MQ=255)                   gATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAg                                                                                                       >  1:641806/1‑140 (MQ=255)                     tCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCg                                                                                                     <  1:447135/140‑1 (MQ=255)                       tGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGaa                                                                                                   >  2:473506/1‑140 (MQ=255)                         tttttGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACCGCCATGTGCGTACCGCTGATACTCTTTAGCGAAtt                                                                                                 >  2:356776/1‑140 (MQ=255)                             tGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATc                                                                                             >  2:517407/1‑140 (MQ=255)                                  cgccgccgATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCcgcg                                                                                        <  1:451404/140‑1 (MQ=255)                                   gccgccgATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGt                                                                                       >  1:524298/1‑140 (MQ=255)                                         gATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCtt                                                                                 <  1:473506/140‑1 (MQ=255)                                            gACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGc                                                                              <  2:720903/140‑1 (MQ=255)                                            gACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGc                                                                              <  2:524298/140‑1 (MQ=255)                                            gACTTCACGGAGGCTGCCTGCGCCATATAGTTTTGCCTCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGc                                                                              <  1:274545/140‑1 (MQ=255)                                                           tCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTcgc                                                               >  2:656073/1‑140 (MQ=255)                                                            ccTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATATATACCGCGTGGGCTTTACCTGCTGAGTATTcgcc                                                              >  2:279052/1‑140 (MQ=255)                                                             cTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTcgccg                                                             <  2:641806/140‑1 (MQ=255)                                                                cgcCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCca                                                          <  2:57019/140‑1 (MQ=255)                                                                  ccAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCcatc                                                        <  1:93131/140‑1 (MQ=255)                                                                  ccAGATAATTTTGCCGCGCCACATCGATACCGCGTTCAGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCcatc                                                        <  1:356776/140‑1 (MQ=255)                                                                     gATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCcatctca                                                     >  1:427888/1‑140 (MQ=255)                                                                     gATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCcatctca                                                     >  2:598920/1‑140 (MQ=255)                                                                             tGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAActc                                             >  1:609390/1‑140 (MQ=255)                                                                                        aTCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACg                                  <  1:517407/140‑1 (MQ=255)                                                                                          cGATATCGCATTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGCGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGcc                                <  2:424133/140‑1 (MQ=255)                                                                                           gATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGccc                               <  2:596442/140‑1 (MQ=255)                                                                                           gATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGccc                               <  2:492881/140‑1 (MQ=255)                                                                                           gATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGccc                               <  1:399425/140‑1 (MQ=255)                                                                                            atatCGCGTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCg                              <  1:53495/140‑1 (MQ=255)                                                                                                  cgTTCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCTATCTCATCTAACTCTGCGCCCAACGCCCGCTGttt                        >  2:569963/1‑140 (MQ=255)                                                                                                    ttCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCGCTGTTTaa                      >  1:498522/1‑139 (MQ=255)                                                                                                    ttCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCGCTGTTTAt                      >  2:50373/1‑140 (MQ=255)                                                                                                    ttCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCGCTGTTTAt                      >  2:478214/1‑140 (MQ=255)                                                                                                    ttCTGAAAACGGGTTTACTTTCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCGCTGTTTAt                      >  2:667542/1‑140 (MQ=255)                                                                                                                       ttCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCGCTGTTTATGTTCTGCGGTAACGATATc   <  2:34799/140‑1 (MQ=255)                                                                                                                       ttCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCGCTGTTTATGTTCTGCGGTAACGATATc   <  2:100278/140‑1 (MQ=255)                                                                                                                        tCGCCTGACTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGTCCAACGCCCGCTGTTTATGTTCTGCGGTAACGATATcc  >  1:131995/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                     GCTATCCAGCTGGCTCTGGATCTGTTTTTGTTCCGCCGCCGATGACTTCACGGAGGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTGAAAACGGGTTTACTTTCGCCTGGCTGCCACGTGCGTAACGCTGATACTCTTTAGCGAATTTATCCCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTGCGCCCAACGCCCGCTGTTTATGTTCTGCGGTAACGATATCC  >  NC_000913/3753518‑3753776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑