Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,756,083	A→G	G143G (GGT→GGC)	aldB ←	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,756,083	0	A	G	100.0%	122.3 / NA	35	G143G (GGT→GGC)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/14);  total (21/14)

GCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTG  >  NC_000913/3755948‑3756217                                                                                                                                        |                                                                                                                                       gCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCtt                                                                                                                                    >  2:114618/1‑140 (MQ=255)    cgccgcCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCTACGGTTTAGCTCTCCACTTCACTGATCCCGCCTTCCt                                                                                                                                 >  1:237131/1‑140 (MQ=255)     gccgccAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTg                                                                                                                                <  1:31868/140‑1 (MQ=255)     gccgccAGCGCGGGAGACATTTTCCAGCTCGACATTAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTg                                                                                                                                <  2:333051/140‑1 (MQ=255)       cgccAGCGCGGGAGCCATTTTCCAGCTCGCCATCATCAGTGGGAAGTTTCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTgcg                                                                                                                              <  2:279435/140‑1 (MQ=255)       cgccAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTgcg                                                                                                                              <  1:89943/140‑1 (MQ=255)         ccAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGcc                                                                                                                            <  2:583237/140‑1 (MQ=255)                 ggAGCCATTTTCCAGATCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATaca                                                                                                                    <  1:302758/140‑1 (MQ=255)                    gCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGa                                                                                                                 >  2:158051/1‑140 (MQ=255)                                   gCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTGACTGATCCGGCCTTCCAGCGCCCGAATACGAGAGGCGAAATAGCGGaa                                                                                                  >  2:236380/1‑140 (MQ=255)                                     cATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAAt                                                                                                >  2:117235/1‑140 (MQ=255)                                                   aGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGc                                                                                  >  1:126656/1‑140 (MQ=255)                                                             gATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCg                                                                        <  2:126656/140‑1 (MQ=255)                                                                aaTCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCgcag                                                                     >  2:371367/1‑140 (MQ=255)                                                                  tCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCgcagca                                                                   <  1:410356/140‑1 (MQ=255)                                                                              cGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGa                                                       >  2:435493/1‑140 (MQ=255)                                                                               gCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGaa                                                      <  1:371367/140‑1 (MQ=255)                                                                               gCCTAACGGTTCATGGAAATGAGAGGCCACGATATAGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGaa                                                      <  2:331368/140‑1 (MQ=255)                                                                                   aaCGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCACAGCACTGGTTTCGCGAATggg                                                  >  1:331368/1‑140 (MQ=255)                                                                                     cGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTcgcg                                                        >  1:495363/1‑132 (MQ=255)                                                                                     cGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTcgcg                                                        <  2:495363/132‑1 (MQ=255)                                                                                          cATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCg                                           >  2:176784/1‑140 (MQ=255)                                                                                          cATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCg                                           >  1:130918/1‑140 (MQ=255)                                                                                          cATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCg                                           >  1:542640/1‑140 (MQ=255)                                                                                             ggAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTa                                        <  1:158051/140‑1 (MQ=255)                                                                                                 aTGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATccc                                    >  1:398808/1‑140 (MQ=255)                                                                                                  tGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCa                                   <  1:508946/140‑1 (MQ=255)                                                                                                             ggTTTCGCTATCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTg                        >  2:686355/1‑140 (MQ=255)                                                                                                                       tCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAg              >  1:454271/1‑140 (MQ=255)                                                                                                                       tCAACTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGGTTCGCGAAAGGGGTTGCCGTTATCCTAGGTCTCAGCTGTCGCTAACAg              >  2:205622/1‑140 (MQ=255)                                                                                                                         aaCTTCACTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCt            >  2:83537/1‑140 (MQ=255)                                                                                                                           cTTCACTGATCCCGCATTCCTGCGCTCGAATACGCGAAGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCg          <  1:236380/140‑1 (MQ=255)                                                                                                                                cTGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTATATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGtt     <  1:134270/140‑1 (MQ=255)                                                                                                                                 tGATCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGttt    >  2:13881/1‑140 (MQ=255)                                                                                                                                   aTCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTg  >  1:478851/1‑140 (MQ=255)                                                                                                                                   aTCCCGCCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTg  >  1:17094/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTG  >  NC_000913/3755948‑3756217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑