Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,863,379 T→C L75L (TTA→TTG)  glvC ← putative PTS enzyme II component GlvC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,863,3790TC98.2% 190.6 / NA 55L75L (TTA→TTG) glvCputative PTS enzyme II component GlvC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (24/30);  minor base G (0/1);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGTAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGTCAGTGATTCCCCGACAAACATCGGGTTTTGCAGCAAG  >  NC_000913/3863243‑3863514
                                                                                                                                        |                                                                                                                                       
cgTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAACAAGCCACTGCGCTTCCTTAACAAGGCCAATGGGCaaa                                                                                                                                      >  2:268939/1‑140 (MQ=255)
  tCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAAcc                                                                                                                                    >  2:665232/1‑140 (MQ=255)
  tCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAAcc                                                                                                                                    >  2:148730/1‑140 (MQ=255)
    cTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGa                                                                                                                                  <  1:676968/140‑1 (MQ=255)
     tGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCACACAAGCACACCCATGCGCTTGCTTAACAAGACCAATGGGCAAACCGAc                                                                                                                                 >  2:214374/1‑140 (MQ=255)
        gTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGc                                                                                                                              >  1:512694/1‑140 (MQ=255)
             aTCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCaaaaa                                                                                                                         >  2:63734/1‑140 (MQ=255)
                  cGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGc                                                                                                                    <  2:592632/140‑1 (MQ=255)
                   gCCGAAGTAGCTTCCCCAGGTCTTTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCg                                                                                                                   <  2:304367/140‑1 (MQ=255)
                      gAAGTAGCTTCCCCAGTTCATTCCCATCGCCTTGAGGAATTAGTTCCACGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCa                                                                                                                <  2:348574/140‑1 (MQ=255)
                       aaGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCat                                                                                                               >  2:714103/1‑140 (MQ=255)
                        aGTAGCTTCCCCAGGTCTTTCCTATCGCGTTAATGTAATCGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCata                                                                                                              <  2:354400/140‑1 (MQ=255)
                        aGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCata                                                                                                              <  1:434006/140‑1 (MQ=255)
                        aGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCata                                                                                                              <  2:47177/140‑1 (MQ=255)
                        aGTAGCTTCCCCAGGCCATTCCCATCGCGTTGATGAACTAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCata                                                                                                              <  1:321522/140‑1 (MQ=255)
                            gCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTAc                                                                                                          >  2:15736/1‑140 (MQ=255)
                            gCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTAc                                                                                                          <  1:714103/140‑1 (MQ=255)
                                ccccAGGTCATTCCCATCGCGTCGAGGAAATAGTTCCCGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGaa                                                                                                      <  1:214374/140‑1 (MQ=255)
                                ccccAGGTCATTCCCATCGCGTAAATGAAATCGTTTCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGaa                                                                                                      <  2:214975/140‑1 (MQ=255)
                                 cccAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAAt                                                                                                     >  1:58950/1‑140 (MQ=255)
                                          ttCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCaa                                                                                            >  1:521949/1‑140 (MQ=255)
                                           tCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAAc                                                                                           >  1:46519/1‑140 (MQ=255)
                                             ccATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCg                                                                                         <  2:78639/140‑1 (MQ=255)
                                               aTCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCGCCGCCAGACAAGCACGCCCCTGAGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATCCCGTCCAACCGcc                                                                                       <  2:60321/140‑1 (MQ=255)
                                               aTCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGcc                                                                                       <  2:55244/140‑1 (MQ=255)
                                               aTCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGcc                                                                                       <  2:46519/140‑1 (MQ=255)
                                               aTCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGcc                                                                                       <  2:58950/140‑1 (MQ=255)
                                               aTCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGcc                                                                                       <  1:418888/140‑1 (MQ=255)
                                               aTCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGcc                                                                                       <  2:23102/140‑1 (MQ=255)
                                               aTCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGcc                                                                                       <  2:717562/140‑1 (MQ=255)
                                                 gagacagatgaAATATTTCCAGGTCCGGACACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCct                                                                                     <  1:242107/134‑1 (MQ=255)
                                                 cgcgTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCct                                                                                     <  2:504051/140‑1 (MQ=255)
                                                 cgcgTTGACGAAATAGTGCCAGGTCAGGAATCTGCTCATGACCGCCAGGCAAGCCCGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCct                                                                                     <  2:341157/140‑1 (MQ=255)
                                                        atgaAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCaat                                                                              <  1:665232/140‑1 (MQ=255)
                                                         tgaAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCaata                                                                             <  2:512694/140‑1 (MQ=255)
                                                                   ccAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTAAGGAGTACAGTCCAACCGCCCTATTCAATAAtgctgtctc                                                                   >  2:242107/1‑133 (MQ=255)
                                                                      ggTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGAttt                                                                >  1:283576/1‑140 (MQ=255)
                                                                      ggTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTAAATAATGTGTACGAttt                                                                >  2:401915/1‑140 (MQ=255)
                                                                       gTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTg                                                               >  2:487574/1‑140 (MQ=255)
                                                                        tCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTgc                                                              <  1:15736/140‑1 (MQ=255)
                                                                         cAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTAAATAATGTGTACGATTTgcg                                                             <  1:401915/140‑1 (MQ=255)
                                                                                 tCACCATCACCGCCAGACAAGCGCGGCCCGGCGCTTGGTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATaaa                                                     <  1:253297/140‑1 (MQ=255)
                                                                                 tCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATaaa                                                     <  1:699315/140‑1 (MQ=255)
                                                                                          ccGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGa                                            >  2:575091/1‑140 (MQ=255)
                                                                                           cGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGAt                                           >  1:177999/1‑140 (MQ=255)
                                                                                           cGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGAt                                           <  1:148730/140‑1 (MQ=255)
                                                                                               aGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGt                                       >  2:414538/1‑140 (MQ=255)
                                                                                               aGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGt                                       >  2:373549/1‑140 (MQ=255)
                                                                                               aGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGt                                       <  1:640525/140‑1 (MQ=255)
                                                                                                         gCCCCTGCGCTTGCTTAGCAAGCCCAATGGGGAAACCGATAGCAAAAAGCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGTCAGTGATTcc                             <  2:283576/140‑1 (MQ=255)
                                                                                                                     gcttAGCAAGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATATACTGTTCGGATAGGGCAGTGACTCCCCGACAAACATc                 >  1:208316/1‑140 (MQ=255)
                                                                                                                             aGGCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGTCAGTGATTCCCCGACAAACATCGGGTTTTg         >  1:7530/1‑140 (MQ=255)
                                                                                                                               gCCAATGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGTCAGTGATTCCCCGACAAACATCGGGTTTTgca       >  1:87831/1‑140 (MQ=255)
                                                                                                                                  aaTGGGCAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGTCAGTGATTCCCCGACAAACATCGGGTTTTgcagca    >  1:685300/1‑140 (MQ=255)
                                                                                                                                    tGGGCAAACCGACAGCAAAAATCAGCAGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGTCAGTGATTCCCCGACAAACATCGGGTTTTGCAGCAAg  >  1:608525/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACCATCACCGCCAGACAAGCACGCCCCTGCGCTTGCTTAGCAAGGCCAATGGGTAAACCGACAGCAAAAATCAGCGGCATATTACGGAATACCGTCCAACCGCCCTCTTCAATAATGTGTACGATTTGCGCGAATAAACTGTTCGGATCGGTCAGTGATTCCCCGACAAACATCGGGTTTTGCAGCAAG  >  NC_000913/3863243‑3863514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑