Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,881,409	T→C	I305V (ATC→GTC)	dnaN ←	beta sliding clamp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,881,409	0	T	C	100.0%	133.4 / NA	40	I305V (ATC→GTC)	dnaN	beta sliding clamp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/20);  total (20/20)

CAATCTGCACGCTGGAAACCGAATCGGTCAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGATCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGCCTGCTTGAGCAGATCGCA  >  NC_000913/3881274‑3881544                                                                                                                                        |                                                                                                                                        cAATCTGCACGCTGGAAACCGAATCGGTCAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACctct                                                                                                                                     >  2:660206/1‑140 (MQ=255) cAATCTGCACGCTGGAAACCGAATCGGTCAACATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACctct                                                                                                                                     >  2:567724/1‑140 (MQ=255)    tCTGCACGCTGGAAACCGAATCGGTCAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTcc                                                                                                                                  <  2:142080/140‑1 (MQ=255)            cTGGAAACCGAATCGGTCAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCttcttc                                                                                                                          <  2:80995/140‑1 (MQ=255)               gAAACCGAATCGGTCAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTg                                                                                                                       <  2:482173/140‑1 (MQ=255)                         cGGTCAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGgttg                                                                                                             >  2:635960/1‑140 (MQ=255)                         cGGTCAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGgttg                                                                                                             >  1:735476/1‑140 (MQ=255)                         cGGTCAGCATCATGCGGACGTTGTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTGCCGCTTCTTCCTGTTCCGGgttg                                                                                                             >  2:520044/1‑140 (MQ=255)                             cAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTgg                                                                                                         <  1:707374/140‑1 (MQ=255)                                catcatGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCgg                                                                                                      <  1:520044/140‑1 (MQ=255)                                catcatGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCgg                                                                                                      <  1:635960/140‑1 (MQ=255)                                         gACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGAACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCa                                                                                             >  2:38703/1‑140 (MQ=255)                                                     tttCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGc                                                                                 <  2:533749/140‑1 (MQ=255)                                                      ttCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCAGGGATGTTGGCGATGATGTTCAGCTGGTTTTCGCt                                                                                >  2:219249/1‑140 (MQ=255)                                                          gcgcGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACa                                                                            <  1:567724/140‑1 (MQ=255)                                                            gcgTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATa                                                                          <  2:512600/140‑1 (MQ=255)                                                                   gAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTAGTTTTCACTGACATAAAGACGt                                                                   >  1:330649/1‑140 (MQ=255)                                                                          cAGCACATAACTGACGTTGAAGCCGATTTCCATCTACGCACCGCTATAAGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTAcgccgc                                                            <  2:297152/140‑1 (MQ=255)                                                                                  aaCTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTtc                                                    >  1:637556/1‑140 (MQ=255)                                                                                   aCTGACGTTGAAGCCGATTTCCCTCCCCGCACCGCTACAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTtct                                                   <  1:295537/140‑1 (MQ=255)                                                                                    cTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTtctc                                                  >  2:136793/1‑140 (MQ=255)                                                                                     tGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGAACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCg                                                 <  1:38703/140‑1 (MQ=255)                                                                                       aCGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGCTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGtt                                               >  1:687917/1‑140 (MQ=255)                                                                                                       ccATCTCCGCACCGCTTCAGGTTACGTCGAGTACCTCTTCCGCTTCTTTCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCcgcg                               <  2:330649/140‑1 (MQ=255)                                                                                                            tcCGCACCGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGc                          <  1:136793/140‑1 (MQ=255)                                                                                                                 aCCGCTATAGGTAACCTCGAGGACCACTTACGCTTCTCCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACg                     <  1:219249/140‑1 (MQ=255)                                                                                                                   cGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGcc                   <  1:94591/140‑1 (MQ=255)                                                                                                                   cGCTATAGGTAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGcc                   <  2:385260/140‑1 (MQ=255)                                                                                                                   cGCTATAGGTAACGTAGAGGACGTCTTCCCCTTCTTCGTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGcc                   <  1:283314/140‑1 (MQ=255)                                                                                                                            tAACGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGCCTGCTTGAGc          <  2:735476/140‑1 (MQ=255)                                                                                                                             aaCGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACg                     >  1:666310/1‑128 (MQ=255)                                                                                                                             aaCGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACg                     <  2:666310/128‑1 (MQ=255)                                                                                                                             aaCGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGCCTGCTTGAGCa         >  1:25870/1‑140 (MQ=255)                                                                                                                             aaCGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGCCTGCTTGAGCa         >  1:504982/1‑140 (MQ=255)                                                                                                                             aaCGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGGTAGTGAGAATCGCCGCGCGAGAAAACGCCTGCTTGAGCa         >  2:218768/1‑140 (MQ=255)                                                                                                                               cGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGCCTGCTTGAGCAGa       >  1:647062/1‑140 (MQ=255)                                                                                                                               cGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGCCTGCTTGAGCAGa       >  2:75256/1‑140 (MQ=255)                                                                                                                               cGTCGAGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAACTTCTCGTTAGAGAGAAACGCCGCGCCAGCAAACGCCTGCTTGAGCAGa       >  2:293134/1‑140 (MQ=255)                                                                                                                                    aGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGCCTGCTTGAGCAGATCGca  >  1:15949/1‑140 (MQ=255)                                                                                                                                    aGGACCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGACAATTGCCCCGCGAGCATACGCCTGCTTGAGCAGATCGca  >  2:248222/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CAATCTGCACGCTGGAAACCGAATCGGTCAGCATCATGCGGACGTTTTCGCATTTCAGCGCGTTCAGAACATCCAGCACATAACTGACGTTGAAGCCGATTTCCATCTCCGCACCGCTATAGGTAACGTCGAGGATCTCTTCCGCTTCTTCCTGTTCCGGGTTGTTGGCGGTGATTTTCAGCTGGTTTTCGCTGACATAAAGACGTACGCCGCGGAATTTCTCGTTAGAGAGAATCGCCGCGCGAGCAAACGCCTGCTTGAGCAGATCGCA  >  NC_000913/3881274‑3881544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑