Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,894,592	G→A	A238T (GCC→ACC)	yieE →	putative phosphopantetheinyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,894,592	0	G	A	100.0%	153.7 / NA	46	A238T (GCC→ACC)	yieE	putative phosphopantetheinyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/27);  total (19/27)

TGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGT  >  NC_000913/3894455‑3894719                                                                                                                                          |                                                                                                                                tGCTGGTGTGGTCCGTGCTGGTGACGCCCACCATTTAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTAcc                                                                                                                               <  2:209457/140‑1 (MQ=255)    tGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCaa                                                                                                                            <  1:662935/140‑1 (MQ=255)    tGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCaa                                                                                                                            <  1:568405/140‑1 (MQ=255)        gtgGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAActct                                                                                                                        >  1:987/1‑140 (MQ=255)                tGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTg                                                                                                                >  1:682108/1‑140 (MQ=255)                tGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTg                                                                                                                >  1:473103/1‑140 (MQ=255)                  gCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGaa                                                                                                              <  1:176762/140‑1 (MQ=255)                     gTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGc                                                                                                           >  1:107497/1‑140 (MQ=255)                               cGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCa                                                                                                 <  2:144630/140‑1 (MQ=255)                                          cTCAGTGTTTGGGAGTTCGATGGCAAACACGACAGTAAATGCCTGCCGTATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGAc                                                                                      <  2:346422/140‑1 (MQ=255)                                          cTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATa                                                                                         <  2:506004/137‑1 (MQ=255)                                          cTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATa                                                                                         >  1:506004/1‑137 (MQ=255)                                                gtCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACcac                                                                                <  1:64716/140‑1 (MQ=255)                                                  cTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAg                                                                              <  2:107497/140‑1 (MQ=255)                                                  cTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAg                                                                              <  2:473103/140‑1 (MQ=255)                                                    gggAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGa                                                                            <  1:146385/140‑1 (MQ=255)                                                         ttAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGtcat                                                                       >  1:136015/1‑140 (MQ=255)                                                         ttAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGtcat                                                                       >  2:445963/1‑140 (MQ=255)                                                         ttAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGtcat                                                                       <  1:184254/140‑1 (MQ=255)                                                         ttAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTATACCGTGAAGGCATTTTCGCCAAATCGATAAACAACCACAGGAGtcat                                                                       >  2:281185/1‑140 (MQ=255)                                                                 cAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTg                                                               >  1:558775/1‑140 (MQ=255)                                                                   aaCACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGaa                                                             >  1:421943/1‑140 (MQ=255)                                                                   aaCACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGaa                                                             >  2:48416/1‑140 (MQ=255)                                                                          cTGGAAAAGCCTGCCTGATATTTACAGCCTCGCCAACCATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGc                                                      <  1:195000/140‑1 (MQ=255)                                                                                 aGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAggtggt                                               <  2:682108/140‑1 (MQ=255)                                                                                   ccTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGgtta                                             <  1:445963/140‑1 (MQ=255)                                                                                      gCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGgttacgt                                          <  2:987/140‑1 (MQ=255)                                                                                       ccGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGgttacgtt                                         >  2:62167/1‑140 (MQ=255)                                                                                       ccGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGgttacgtt                                         <  2:558775/140‑1 (MQ=255)                                                                                            tatTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGgtta                                             >  1:684612/1‑131 (MQ=255)                                                                                            tatTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGgtta                                             <  2:684612/131‑1 (MQ=255)                                                                                                 acaGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTAGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGc                               >  1:517270/1‑140 (MQ=255)                                                                                                        cgcCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGgttacgtt                                         >  2:105702/1‑123 (MQ=255)                                                                                                        cgcCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGgttacgtt                                         <  1:105702/123‑1 (MQ=255)                                                                                                        cgcCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCa                        >  2:77317/1‑140 (MQ=255)                                                                                                        cgcCAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCa                        >  1:510512/1‑140 (MQ=255)                                                                                                          ccAACAATCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCaaa                      <  1:383333/140‑1 (MQ=255)                                                                                                           caacaaTCCTACCAGCCGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAg                     <  2:510512/140‑1 (MQ=255)                                                                                                            aacaaTCCTACCAGCTGGATGATGCTTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAgg                    <  1:281185/140‑1 (MQ=255)                                                                                                                          ccGGATGATGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCa      >  1:642346/1‑140 (MQ=255)                                                                                                                             gatgatGCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATgg   <  1:413218/140‑1 (MQ=255)                                                                                                                              atgatgCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCa      >  1:594092/1‑136 (MQ=255)                                                                                                                              atgatgCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCa      <  2:594092/136‑1 (MQ=255)                                                                                                                              atgatgCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGt  <  2:381584/140‑1 (MQ=255)                                                                                                                              atgatgCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGt  <  1:671505/140‑1 (MQ=255)                                                                                                                              atgatgCGTTTTACCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGt  <  2:136015/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                TGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGT  >  NC_000913/3894455‑3894719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑