Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,915,903	T→C	I24V (ATC→GTC)	atpC ←	ATP synthase F1 complex subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,915,903	0	T	C	100.0%	133.5 / NA	40	I24V (ATC→GTC)	atpC	ATP synthase F1 complex subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

ACAGATAGATAAACTCTTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGATTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGA  >  NC_000913/3915777‑3916036                                                                                                                               |                                                                                                                                      acagaTAGATAAACTCTTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCgaccaga                                                                                                                          >  2:661705/1‑140 (MQ=255) acagaTAGATAAACTCTTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCgaccaga                                                                                                                          >  1:64892/1‑140 (MQ=255)    gatagataAACTCTTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCgaccagacca                                                                                                                       <  1:461762/140‑1 (MQ=255)          taAACTCTTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAAc                                                                                                                 <  2:28455/140‑1 (MQ=255)          taAACTCTTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGGAGATCCCCAGTTCACCTTCGCTACCCGTTCCCTAGGTTTTCTCTACCAGACCAGAGAAc                                                                                                                 >  1:231897/1‑140 (MQ=255)           aaaCTCTTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGGAGATCCCCCGTTCACCTTCGCTCCCCGTTAACTGAACGTTCTCGACCAGACCAGAGAACa                                                                                                                >  1:275002/1‑140 (MQ=255)               tctTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATttg                                                                                                            >  1:551651/1‑140 (MQ=255)               tctTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGGAGATCCCCAGTTCACACTCGCTACCCGTAACCGTGACTTTTGCGACCAAACCAGAGAACAtttt                                                                                                            >  2:241543/1‑139 (MQ=255)                   cGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGAT‑CCTAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGct                                                                                                       >  1:145887/1‑140 (MQ=255)                    gTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGct                                                                                                       <  2:98147/140‑1 (MQ=255)                           tGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCt                                                                                                >  1:565694/1‑140 (MQ=255)                           tGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCCGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCCCTACCCGTTACCTGGGCTTTCTCGACCAGACCAGAGAAAATTTGTTGCTCTGCGCt                                                                                                >  2:365589/1‑140 (MQ=255)                             cTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTga                                                                                              <  1:661705/140‑1 (MQ=255)                              tGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTgac                                                                                             <  1:49154/140‑1 (MQ=255)                                          cGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGGAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCCCTGCGGTGACGACGTCCAggtg                                                                                 >  2:329632/1‑140 (MQ=255)                                            aaTCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAggtggt                                                                               <  1:86763/140‑1 (MQ=255)                                                  aCCAGGCTTAATGGCGGTGAGCAGCGGAGTGTGGGCCAAGAAGGTCCCCAATTCCACCTCGCTACCCGTTACCTCGACTTTCTCGACCAAACAAGAGAACATTTGTTAGTCTGCGCTGAAAGCGTCCCgggggttaaaca                                                                         >  2:400023/1‑134 (MQ=255)                                                           aaTGGCGGTGAGCAGCGGTGCGGGGACAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCatat                                                                <  1:241543/140‑1 (MQ=255)                                                            aTGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAAACCAGAGAACATTTGTTGCTGTGCACTGACGACGTCCTGGGGGTAAGTCATTGCCATATc                                                               >  2:289870/1‑140 (MQ=255)                                                            aTGGCGGTGAGCAGCGGGGCGTGGCCAGGGGAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATc                                                               >  2:496442/1‑140 (MQ=255)                                                                cGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCAccc                                                           <  1:396960/140‑1 (MQ=255)                                                                 ggTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCCGACCAGAGAACATTTGTTGCTCTGCGCCGGCGACGTCCAGGGGGTAAGTCATTGCCATATCAcccc                                                          >  2:75780/1‑139 (MQ=255)                                                                  gTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTc                                                         >  2:76240/1‑140 (MQ=255)                                                                  gTGAGCAGCGGTGCGTGGCCAGGGGAGATCCCCCGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGAGCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTc                                                         >  2:650274/1‑140 (MQ=255)                                                                          cGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAg                                                 >  2:622878/1‑140 (MQ=255)                                                                                  ggCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTaaa                                         <  2:64892/140‑1 (MQ=255)                                                                                       gggTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttt                                    <  2:565694/140‑1 (MQ=255)                                                                                       gggTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttt                                    >  2:520711/1‑140 (MQ=255)                                                                                         gTAGAT‑CCTAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTg                                 <  2:145887/140‑1 (MQ=255)                                                                                             aTCCCCAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCGTCCGATAAAGGCGATAAAGTTTTTTGGCt                              >  1:211940/1‑140 (MQ=255)                                                                                                  cAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTc                         >  2:113393/1‑140 (MQ=255)                                                                                                  cAGTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTc                         <  2:551651/140‑1 (MQ=255)                                                                                                    gTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGAGCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCca                       <  1:650274/140‑1 (MQ=255)                                                                                                    gTTCACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCca                       <  1:76240/140‑1 (MQ=255)                                                                                                       cACCTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCAATATCACCGTCCAATTGAGGCGTTAGAGTTTTTTGGCTTTTTCCACAg                    >  2:234058/1‑140 (MQ=255)                                                                                                          cTTCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGctt                 >  2:532700/1‑140 (MQ=255)                                                                                                            tCGCTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCA‑‑‑GGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCg            >  2:657358/1‑140 (MQ=255)                                                                                                               cTACCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCg            >  2:114845/1‑140 (MQ=255)                                                                                                                 aCCCGTTACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCA‑‑‑GGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGa       <  1:657358/140‑1 (MQ=255)                                                                                                                      ttACCTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAAc     <  1:114845/140‑1 (MQ=255)                                                                                                                         ccTGGACTTTCTCGACCTGACCCGAGAACATCTGTCGCTCTGCGCTGAGGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGa  <  2:211940/140‑1 (MQ=255)                                                                                                                         ccTGGACTTTCTCGACCAGACCTGAGAACATTTGTGGCTCTGCGCTGATGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGa  <  2:275002/140‑1 (MQ=255)                                                                                                                         ccTGGACTTTCTCGACCAGACCAGATAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGa  <  1:289870/140‑1 (MQ=255)                                                                                                                         ccTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGa  <  1:554180/140‑1 (MQ=255)                                                                                                                         ccTGGACTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGa  <  1:496442/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                      ACAGATAGATAAACTCTTCGTGACCGTGCTGTTTCACGATGCGAATCATACCAGGCTTAATGGCGGTGAGCAGCGGTGCGTGGCCAGGGTAGATCCCCAGTTCACCTTCGCTACCCGTTACCTGGATTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGA  >  NC_000913/3915777‑3916036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑