Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,935,976 T→C L230P (CTG→CCG)  rbsC → ribose ABC transporter membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,935,9760TC97.4% 120.5 / NA 38L230P (CTG→CCG) rbsCribose ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (24/13);  minor base G (1/0);  total (25/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATGCTGCATCACACGCGTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCTGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCG  >  NC_000913/3935852‑3936108
                                                                                                                            |                                                                                                                                    
aTGCTGCATCACACGCGTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCgg                                                                                                                       <  1:320758/140‑1 (MQ=255)
  gctgcATCACACGCGTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCCTCTCTTCTCTTTGTGGGCTGCCGGCATCGCTGGCCGGGa                                                                                                                     >  2:320758/1‑140 (MQ=255)
  gctgcATCACACGCGTCTGGCGCGTTACATCTACGCGCTGGGCGGCAACGAAACGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGCCTATTCTCTTTGGGTTCTGCCGGCATCGCTGGCCgggg                                                                                                                     >  2:713964/1‑139 (MQ=255)
      cATCACACGCGTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTCTTCTCTTAGTGGTCTGACGGCTTCGCTGGCCGGGATCAt                                                                                                                 >  2:225094/1‑140 (MQ=255)
      cATCACACGCGTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTGGTGGTCTGCCGGCCTCGCTGGCCGGGATCAt                                                                                                                 >  2:236034/1‑140 (MQ=255)
      cATCACACGCGTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGGCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATACTCTTTGCGGTCTGCCGGCATCGCTGGCCGGGATCAt                                                                                                                 >  1:319290/1‑140 (MQ=255)
              gcgTCTGGGGCGTTACATCTACGCGCTGGGCGGCGACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTgg                                                                                                         <  2:377356/140‑1 (MQ=255)
                gTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGgcg                                                                                                       <  2:538870/140‑1 (MQ=255)
                gTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGgcg                                                                                                       >  1:705377/1‑140 (MQ=255)
                  cTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTATCTTTGGGGTCTGCCGACCTCGCTGACAGGGCACATTGAATTGGcgcg                                                                                                     >  2:321057/1‑140 (MQ=255)
                          ttACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTctcct                                                                                             >  1:63980/1‑140 (MQ=255)
                          ttACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTctcct                                                                                             >  2:504921/1‑140 (MQ=255)
                          ttACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTctcct                                                                                             >  2:660867/1‑140 (MQ=255)
                            aCATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCGGGCATCGCGGGCGGGGATCATTGAAGTGGGGCCACTCtcctcc                                                                                           >  1:450495/1‑140 (MQ=255)
                            aCATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATTGCTGGCCCGGAGCAATGAAGTAGCGCGTCTCtcctcc                                                                                           >  1:329235/1‑140 (MQ=255)
                               tCTACGCGCTGGGCGTCAACGATGCCGCACCGCGTCTATCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGca                                                                                        <  1:225094/140‑1 (MQ=255)
                                cTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGcac                                                                                       <  2:63980/140‑1 (MQ=255)
                                       cTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCAc                                                                                >  1:701864/1‑140 (MQ=255)
                                       cTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCAc                                                                                >  1:22325/1‑140 (MQ=255)
                                                     aGCGGCAACGCGTCTTTGTGGTATCAACGTCTATACAATTAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCt                                                                  <  2:329235/140‑1 (MQ=255)
                                                             cgcgTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGGCTGGCTATGAACTg                                                          >  2:608724/1‑140 (MQ=255)
                                                             cgcgTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTg                                                          <  2:532576/140‑1 (MQ=255)
                                                                        ggTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATtgc                                               <  2:705377/140‑1 (MQ=255)
                                                                         gTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATtgct                                              >  2:447617/1‑140 (MQ=255)
                                                                            tCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATACTATTGCcgcg                                           >  2:82505/1‑140 (MQ=255)
                                                                            tCAACGTCAATAAAAACAAAATCATCGTCCATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCg                                           <  1:273907/140‑1 (MQ=255)
                                                                            ccAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCg                                           <  1:27844/139‑1 (MQ=255)
                                                                              aaCGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCggg                                         >  2:652734/1‑139 (MQ=255)
                                                                                   cAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACACCCCACGGCGGGGACTGGCTCTGAGCTGGATGCTATTTGTTCGGTGGCTc                                    >  2:27844/1‑140 (MQ=255)
                                                                                   cAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAAGCCACGGCGTGGAGTGGATATGAGCTGGATGGTATTGCTGCGGTGGTTc                                    >  2:273907/1‑140 (MQ=255)
                                                                                       aaaatcaaaattATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTggg                                <  2:562356/140‑1 (MQ=255)
                                                                                       aaaatcaaaatcATCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTggg                                <  1:597723/140‑1 (MQ=255)
                                                                                           tcaaaatcaTCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGTGCGTCTCTCCTCCGCACAACCCACGGCGGGGTCTGGCTATGAGCTGGATGCTATTTCTGCGGGGGTTCTggggggg                            >  1:141398/1‑139 (MQ=255)
                                                                                           tcaaaatcaTCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCgg                                          >  1:681847/1‑126 (MQ=255)
                                                                                           tcaaaatcaTCGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCgg                                          <  2:681847/126‑1 (MQ=255)
                                                                                                atcatcGTCTATTCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAg                       >  1:22283/1‑140 (MQ=255)
                                                                                                           ttCTCTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTggcgggcg            >  1:42151/1‑140 (MQ=255)
                                                                                                            tctcTTTGTGGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGGCTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGgcgggcgg           >  2:560613/1‑140 (MQ=255)
                                                                                                                  tgtgGTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAgg     >  1:613828/1‑140 (MQ=255)
                                                                                                                  tgtgGTCTACCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACTCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAgg     <  1:713964/140‑1 (MQ=255)
                                                                                                                     ggTCTGCCGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCg  >  1:182029/1‑140 (MQ=255)
                                                                                                                            |                                                                                                                                    
ATGCTGCATCACACGCGTCTGGGGCGTTACATCTACGCGCTGGGCGGCAACGAAGCGGCAACGCGTCTTTCTGGTATCAACGTCAATAAAATCAAAATCATCGTCTATTCTCTTTGTGGTCTGCTGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCG  >  NC_000913/3935852‑3936108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑