Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,945,556 A→G noncoding (1853/2904 nt) rrlC → 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,945,5560AG97.4% 83.6 / NA 40noncoding (1853/2904 nt)rrlC23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (21/17);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAATGATGGCCAGG  >  NC_000913/3945432‑3945692
                                                                                                                            |                                                                                                                                        
ttGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACACCTGCCCCGTGGCGGAAGGTTCATTGATGGGGTCAgc                                                                                                                           >  1:286833/1‑140 (MQ=255)
                    aGTCGAAGATACCAGCTGGCTGCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCg                                                                                                       <  2:140104/140‑1 (MQ=18)
                                cAGCTGGCTGCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTaa                                                                                           >  1:713892/1‑140 (MQ=17)
                                  gctggctgCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAAc                                                                                         >  2:652207/1‑140 (MQ=17)
                                      gctgCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAAcggcg                                                                                     <  1:81047/140‑1 (MQ=17)
                                         gCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGcc                                                                                  <  2:703708/140‑1 (MQ=17)
                                           aaCTGTTTATTAAAATCACAGCTCTGTGCAAACACGAGAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGt                                                                                <  1:361314/140‑1 (MQ=255)
                                              tGTTTATTAAAAACACAGCACTTTGCAAACACGAATGTGTACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAc                                                                             <  2:286833/140‑1 (MQ=255)
                                                tttATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACta                                                                           >  2:396391/1‑140 (MQ=17)
                                                   attaAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATaa                                                                        <  1:176873/140‑1 (MQ=17)
                                                    ttaAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAAc                                                                       <  1:427469/140‑1 (MQ=17)
                                                        aaaCACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTc                                                                   >  1:685083/1‑140 (MQ=17)
                                                              aGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAgg                                                             >  2:424928/1‑140 (MQ=17)
                                                              aGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAgg                                                             >  1:674288/1‑140 (MQ=17)
                                                               gCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGt                                                            <  1:649129/140‑1 (MQ=17)
                                                               gCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGt                                                            >  1:686590/1‑140 (MQ=17)
                                                                     tgCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAATGGCGGCCGTAACAATAACGGTCCTAAGGTAGCGaa                                                      >  2:217089/1‑140 (MQ=12)
                                                                     tgCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGGCGTAACGATAACGATCCTAAGGTAGCGaa                                                      >  1:254897/1‑140 (MQ=255)
                                                                     tgCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGaa                                                      >  1:454571/1‑140 (MQ=17)
                                                                     tgCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGaa                                                      >  2:23401/1‑140 (MQ=17)
                                                                     tgCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGaa                                                      >  1:80859/1‑140 (MQ=17)
                                                                     tgCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACGATAACGCTCCTAAGGTAGCGaa                                                      >  1:357147/1‑140 (MQ=12)
                                                                     tgCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGGAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGaa                                                      >  2:239292/1‑140 (MQ=17)
                                                                     tgCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGCAAACGCCCGCCGTAACAATAACGGTCCTAAGGTAGCGaa                                                      >  2:315170/1‑140 (MQ=11)
                                                                      gCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGTGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGaaa                                                     >  1:668208/1‑140 (MQ=17)
                                                                          acacGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGGGGCCGTAACTAGAAGGGTCCTGAGGTAGCGAAATTcc                                                 >  1:211863/1‑140 (MQ=11)
                                                                          acacGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTcc                                                 <  1:676311/140‑1 (MQ=17)
                                                                           cacGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCt                                                <  2:685083/140‑1 (MQ=17)
                                                                               aaaGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTc                                            >  2:409743/1‑140 (MQ=17)
                                                                                               gtgtGACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCt                            >  1:36629/1‑140 (MQ=17)
                                                                                                tgtgACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTg                           >  2:147531/1‑140 (MQ=17)
                                                                                                tgtgACGCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTg                           >  2:162798/1‑140 (MQ=17)
                                                                                                      ggcTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTTAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCCAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGaa                     <  2:633461/138‑1 (MQ=255)
                                                                                                      gcctgcctGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGaa                     <  2:496238/140‑1 (MQ=17)
                                                                                                      gCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGaa                     <  2:80859/140‑1 (MQ=17)
                                                                                                      gCCTGCCCGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGaa                     <  1:652207/140‑1 (MQ=17)
                                                                                                      gCCTGCCCGGTGCCGGAAGGTTAATTGATAGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGaa                     <  1:324262/140‑1 (MQ=17)
                                                                                                           cccGGTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCg                <  2:36629/140‑1 (MQ=17)
                                                                                                              ggTGCCGGAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTaa             <  2:409854/140‑1 (MQ=17)
                                                                                                                     gAAGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAATGATGGc      <  2:454571/140‑1 (MQ=17)
                                                                                                                      aaGGTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAATGATGGcc     <  1:483349/140‑1 (MQ=17)
                                                                                                                         gTTGATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAATGATGGCCAgg  <  1:162798/140‑1 (MQ=17)
                                                                                                                            |                                                                                                                                        
TTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTCAGCGCAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAATGATGGCCAGG  >  NC_000913/3945432‑3945692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑