Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,955,349	C→T	L7L (CTG→TTG)	ilvA →	threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,955,349	0	C	T	100.0%	99.9 / NA	30	L7L (CTG→TTG)	ilvA	threonine deaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

GACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCG  >  NC_000913/3955217‑3955485                                                                                                                                     |                                                                                                                                         gACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCgg                                                                                                                                   <  2:636389/140‑1 (MQ=255) gACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTCGGGGGTTTATACTGGCTGACTCGCAACCCTTGTCCgg                                                                                                                                   >  1:294192/1‑140 (MQ=255) gACGCCGAAAAATCGTGAACGTCAGATCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCgg                                                                                                                                   >  2:593876/1‑140 (MQ=255)  aCGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGt                                                                                                                                  <  2:476836/140‑1 (MQ=255)          aaaTCGTGAACGTCAGGTCTCCGTTGCCCTGCGTGATTACGCCAGCCTAGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGa                                                                                                                          <  2:294192/140‑1 (MQ=255)                    cGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGaa                                                                                                                <  1:446884/140‑1 (MQ=255)                    cGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCCGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGaa                                                                                                                <  2:424686/140‑1 (MQ=255)                       cAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAatat                                                                                                             >  2:600702/1‑140 (MQ=255)                         gatctcCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATAttt                                                                                                           <  1:593876/138‑1 (MQ=255)                              ccTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAagag                                                                                                      <  1:391990/140‑1 (MQ=255)                                    cccTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGtgc                                                                                                >  1:170298/1‑140 (MQ=255)                                         cGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgc                                                                                           >  1:600513/1‑140 (MQ=255)                                         cGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCATTGTCCGGTGCTACGGGAGGTGCCGGATATGTAAGAGCAGTGCTgcgc                                                                                           >  2:211542/1‑140 (MQ=255)                                             cTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgcgcgc                                                                                       <  2:170298/140‑1 (MQ=255)                                                             aaCCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAACCTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAggcggc                                                                       <  2:297971/140‑1 (MQ=255)                                                              aCCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGgcggcg                                                                      <  1:140808/140‑1 (MQ=255)                                                              aCCAGCGCCGACAAAGGCGCGGCGCGCGATACATCTAAACTGGGGGGTCAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGgcggcg                                                                      <  2:202056/140‑1 (MQ=255)                                                                         cAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgcc                                                           <  1:696331/140‑1 (MQ=255)                                                                                                        gggggTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACTCCGCTACAAAAAATGGAAAAACTGTCGTCGCGt                            >  1:38350/1‑140 (MQ=255)                                                                                                        gggggTTAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGt                            >  2:698520/1‑140 (MQ=255)                                                                                                         ggggTTAATAATGGCAGACTCGTAACCCTAGTCCGGTTCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTc                           <  1:211542/140‑1 (MQ=255)                                                                                                             ttAATAATGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGa                       >  1:613179/1‑140 (MQ=255)                                                                                                                   atGGCTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGt                 <  1:698520/140‑1 (MQ=255)                                                                                                                       cTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAGTGGGAAAACTGTTGTCGGGTCTTGATAACGTCAtt             >  2:203209/1‑140 (MQ=255)                                                                                                                       cTGACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCAtt             >  2:497258/1‑140 (MQ=255)                                                                                                                         gACTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCt           >  2:722341/1‑140 (MQ=255)                                                                                                                          aCTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTc                           <  1:514552/123‑1 (MQ=255)                                                                                                                          aCTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTc                           >  2:514552/1‑123 (MQ=255)                                                                                                                           cTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTgg         >  2:45459/1‑140 (MQ=255)                                                                                                                           cTCGCAACCCTTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTgg         >  1:178003/1‑140 (MQ=255)                                                                                                                                  ccctTGTCCGGTGCTCCCGAAGCTGCCGAATATTTTAGATCAGTGCTGGGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAgcg  <  2:220861/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCG  >  NC_000913/3955217‑3955485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑