Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,972,840 A→G T107A (ACA→GCA)  rffG → dTDP‑glucose 4,6‑dehydratase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,972,8400AG96.9% 103.1 / NA 32T107A (ACA→GCA) rffGdTDP‑glucose 4,6‑dehydratase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (18/13);  minor base C (0/1);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACGGATGATTTCTTCACC  >  NC_000913/3972703‑3972968
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gCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCACCATTGTCTGGACTTATGCa                                                                                                                                <  2:144673/140‑1 (MQ=255)
 cGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGTCAGAGTTGATTGAAACGAACAGTGTCGGGACTTATGCAt                                                                                                                               >  2:195132/1‑140 (MQ=255)
 cGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCAt                                                                                                                               >  1:475166/1‑140 (MQ=255)
   aTCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGAGTTTATTAAAAGCAACATTGTAGGGACTTATGCATTg                                                                                                                             >  1:236955/1‑140 (MQ=39)
                  cACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCgg                                                                                                              <  2:683334/140‑1 (MQ=255)
                               tGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATg                                                                                                 >  1:176074/1‑140 (MQ=255)
                                 aGCATCAGCCAGACTGTGTCATGCATCTGGCTGCCCAAATCCATGTTGTCCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATgcg                                                                                               <  2:236955/140‑1 (MQ=255)
                                 aGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATgcg                                                                                               >  1:406900/1‑140 (MQ=255)
                                   cATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGGTTACTGGAATGCGCt                                                                                             >  1:326764/1‑140 (MQ=255)
                                   cATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCt                                                                                             >  2:564322/1‑140 (MQ=255)
                                   cATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCt                                                                                             >  1:451016/1‑140 (MQ=255)
                                     tCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGa                                                                                           >  2:484289/1‑140 (MQ=255)
                                                   tCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATaaaaaa                                                                             >  2:642312/1‑140 (MQ=255)
                                                      tGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACCGGAGTGGGCTGACAGAAGATAAAAAATCa                                                                          >  1:310120/1‑140 (MQ=38)
                                                         aTCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCg                                                                       >  1:663458/1‑140 (MQ=255)
                                                                 gCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGttt                                                               >  1:67951/1‑140 (MQ=255)
                                                                  ccGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGtttt                                                              <  1:564322/140‑1 (MQ=255)
                                                                     aaaGCCATGTTGACCGTGCTATTGACGGCCCCGCACCGTTTATTGAAATCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTtcat                                                           <  2:310120/140‑1 (MQ=255)
                                                                        gCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAGTGCGCTGAGGGAAGATAAAGAATCATCGTTCCATTTGcatcat                                                        >  1:455709/1‑140 (MQ=255)
                                                                        gCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTcatcat                                                        <  2:451016/140‑1 (MQ=255)
                                                                          cATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCatat                                                      <  2:663458/140‑1 (MQ=255)
                                                                                    gTTCTATTGACGGCCCGGCAGCGTTTATTGAGACCGACACTGTCGGGAATTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGAc                                            <  2:272698/140‑1 (MQ=255)
                                                                                    gTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACAGTGTCGGGATTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGAc                                            <  2:268717/140‑1 (MQ=255)
                                                                                     ttCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACg                                           <  2:176074/140‑1 (MQ=255)
                                                                                      tCTATTGACGGCCCGGCAGCGTCTACTGATACCAACAATGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGa                                          <  1:243217/140‑1 (MQ=255)
                                                                                         aTTGACGGCCCGGCAGCTTTTACTGAAACCAACATTGTCAGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGt                                       <  2:326764/140‑1 (MQ=255)
                                                                                             aCGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGAGAAAAAATCGGCGGTCCGTTTTCATCATATCTCCACTGACGAAGTatat                                   >  2:243217/1‑140 (MQ=255)
                                                                                                    ggCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGAc                                            >  2:436163/1‑124 (MQ=255)
                                                                                                    ggCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGAc                                            <  1:436163/124‑1 (MQ=255)
                                                                                                    ggCAGCGTTTATTGAAACCAACATTGTCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGAcc                            >  2:462607/1‑140 (MQ=255)
                                                                                                                tGAAACCAACATTGTCGGGACTAATCCATTGCTTGAATCGGCACGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACgg                <  1:195132/140‑1 (MQ=255)
                                                                                                                tGAAACAAACATTTCCGGGACTTATGCATTGCATGCAGCGGCGCGGGCTCACTGCAATGCCCTGACTGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGATGAAGTATATGGTGACCTGCACTCGACgg                <  2:455709/140‑1 (MQ=255)
                                                                                                                              tCGGGACTTATGCATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACGGATGATTTCTTCAcc  >  1:33289/1‑140 (MQ=255)
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GCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACGGATGATTTCTTCACC  >  NC_000913/3972703‑3972968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑