Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,043,282	T→C	F290L (TTC→CTC)	srkA →	stress response kinase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,043,282	0	T	C	97.9%	162.9 / NA	48	F290L (TTC→CTC)	srkA	stress response kinase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (22/25);  minor base G (1/0);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCA  >  NC_000913/4043148‑4043418                                                                                                                                       |                                                                                                                                         tGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCg                                                                                                                                     <  1:119186/140‑1 (MQ=255)   gAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGaa                                                                                                                                   >  2:409349/1‑140 (MQ=255)     aaCTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGaaaa                                                                                                                                 <  1:658942/140‑1 (MQ=255)                aaGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTgg                                                                                                                      <  2:380134/140‑1 (MQ=255)                  gCTTATGAAGAATTTAGTGAGTACGACACCGCTGACATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGtt                                                                                                                    <  2:227892/140‑1 (MQ=255)                  gCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGAGCTGATCCCGCGGTCCCTAAAAATTTCCCGTGGtt                                                                                                                    >  2:215888/1‑140 (MQ=255)                    ttATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTaa                                                                                                                  <  1:161376/140‑1 (MQ=255)                     tATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAAc                                                                                                                 >  1:17192/1‑140 (MQ=255)                       tGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCg                                                                                                               >  1:259661/1‑140 (MQ=255)                             aTTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAg                                                                                                         >  2:630049/1‑140 (MQ=255)                                      gTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGc                                                                                                >  1:149915/1‑140 (MQ=255)                                        tCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTg                                                                                              >  2:641176/1‑140 (MQ=255)                                               cGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTa                                                                                                     <  1:15559/126‑1 (MQ=255)                                               cGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTa                                                                                                     >  2:15559/1‑126 (MQ=255)                                                 cTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACg                                                                                     >  1:555382/1‑140 (MQ=255)                                                 cTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACg                                                                                     >  1:69174/1‑140 (MQ=255)                                                 cTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACg                                                                                     <  2:50703/140‑1 (MQ=255)                                                   gAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGc                                                                                   <  2:726862/140‑1 (MQ=255)                                                      aTCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGAc                                                                                <  2:17192/140‑1 (MQ=255)                                                      aTCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGAc                                                                                <  1:409349/140‑1 (MQ=255)                                                           aCTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACTGGGGAAGATTAGTGACTGCGACAGACGGCGACTTTta                                                                           >  1:293875/1‑140 (MQ=255)                                                           aCTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTta                                                                           >  2:662982/1‑140 (MQ=255)                                                                    aCCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAgg                                                                  >  1:95037/1‑140 (MQ=255)                                                                         tACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGAGGGCGACTTTGATAGAACAGGCaaaa                                                             >  1:330763/1‑140 (MQ=255)                                                                         tACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGC‑‑‑‑‑‑‑‑GATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACa                                                     >  2:13371/1‑140 (MQ=255)                                                                               ccATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTa                                                       <  2:368269/140‑1 (MQ=255)                                                                               ccATGCGTTTGGTTTATTATCTCGCCTGGCTGATGCGGCCTTGGGCTGTTCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTa                                                       <  2:259661/140‑1 (MQ=255)                                                                                  tGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACaa                                                    >  1:440824/1‑140 (MQ=255)                                                                                              attatCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTg                                        <  2:54451/140‑1 (MQ=255)                                                                                              attatCTTGCCTGGCTAATGCCGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGATCCCTCTTTg                                        <  2:149915/140‑1 (MQ=255)                                                                                                   cTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAAtt                                   <  2:555382/140‑1 (MQ=255)                                                                                                     tGCCTGGCTAATGCGTCGTTGGACTCATCCCGCGCTCTCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTaa                                 <  1:215888/140‑1 (MQ=255)                                                                                                     tGCCTGGCTAATGCGGCGTTGGTCTGATCCCACGCGCCCGAAAAATTTTCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTaa                                 <  2:293875/140‑1 (MQ=255)                                                                                                     tGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTaa                                 <  2:60129/140‑1 (MQ=255)                                                                                                         tGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACAcc                             <  2:619120/140‑1 (MQ=255)                                                                                                         tGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACAcc                             >  1:612261/1‑140 (MQ=255)                                                                                                         tGGCTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACAcc                             >  1:551485/1‑140 (MQ=255)                                                                                                            cTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTAt                          <  2:551485/140‑1 (MQ=255)                                                                                                            cTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTAt                          >  2:375753/1‑140 (MQ=255)                                                                                                            cTAATGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTAt                          >  1:690881/1‑140 (MQ=255)                                                                                                                tGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTAt                      <  2:440824/140‑1 (MQ=255)                                                                                                                tGCGGCGTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTAt                      >  1:623620/1‑140 (MQ=255)                                                                                                                   ggcgTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTaa                   <  1:123492/140‑1 (MQ=255)                                                                                                                   ggcgTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTaa                   <  1:630049/140‑1 (MQ=255)                                                                                                                     cgTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGTGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATc                 >  2:574882/1‑140 (MQ=255)                                                                                                                     cgTTGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATc                 <  1:641176/140‑1 (MQ=255)                                                                                                                       ttGGGCTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCgg               <  1:684406/140‑1 (MQ=255)                                                                                                                            cTGATCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGgagaga          <  1:601862/140‑1 (MQ=255)                                                                                                                               aTCCCGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGACTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTa       >  1:697510/1‑140 (MQ=255)                                                                                                                                  ccGCGCTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGTGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGAt    <  1:574882/140‑1 (MQ=255)                                                                                                                                    gcgcTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCa  <  1:13371/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCA  >  NC_000913/4043148‑4043418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑