Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,069,512	T→C	H297R (CAC→CGC)	yihR ←	putative aldose 1‑epimerase YihR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,069,512	0	T	C	100.0%	197.1 / NA	55	H297R (CAC→CGC)	yihR	putative aldose 1‑epimerase YihR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/34);  total (21/34)

GCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCACCCAGAGTTGATCGG  >  NC_000913/4069384‑4069647                                                                                                                                 |                                                                                                                                        gctgTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCt                                                                                                                              <  1:714855/140‑1 (MQ=255)    gTTTTTAACTTAATACATTGAAGATTCATAATGTTGCGTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGAt                                                                                                                           <  1:261064/140‑1 (MQ=255)      ttttAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTc                                                                                                                         <  1:452650/140‑1 (MQ=255)      ttttAACTTAATAAAGTGAAGAAACATAAGGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTc                                                                                                                         <  1:329929/140‑1 (MQ=255)         tAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAg                                                                                                                      <  2:100086/140‑1 (MQ=255)          aaCTTAATATATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGt                                                                                                                     <  2:415205/140‑1 (MQ=255)          aaCTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGt                                                                                                                     <  1:574229/140‑1 (MQ=255)               aataaATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCTGTAAATATTAAAAAACCGGCGGCGCCGTTTTCCTGATTCCAGtaataa                                                                                                                >  2:261064/1‑140 (MQ=255)               aataaATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGtaataa                                                                                                                >  2:382119/1‑140 (MQ=255)                ataaATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGtaataat                                                                                                               <  1:382119/140‑1 (MQ=255)                      tGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCg                                                                                                         >  2:363151/1‑140 (MQ=255)                      tGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCg                                                                                                         <  1:530294/140‑1 (MQ=255)                       gAAGATACATAATGTTGGCTTTAGCGAGTGAAATGGTCTGCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGa                                                                                                        <  2:223523/140‑1 (MQ=255)                       gAAGATACATAATGTTGCCTTTAGCGAGTGACATGTTCTACAATGATTGTGACCGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGa                                                                                                        <  2:246458/140‑1 (MQ=255)                           aTACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATAcc                                                                                                    >  2:697026/1‑140 (MQ=255)                           aTACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATAcc                                                                                                    <  1:697026/140‑1 (MQ=255)                               aTAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGaa                                                                                                >  1:47767/1‑140 (MQ=255)                               aTAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGaa                                                                                                >  2:169603/1‑140 (MQ=255)                                      tGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAgg                                                                                         >  2:598225/1‑140 (MQ=255)                                       gCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGc                                                                                        >  2:735281/1‑140 (MQ=255)                                            tAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAg                                                                                   <  1:169603/140‑1 (MQ=255)                                             aGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAgg                                                                                  >  2:448606/1‑140 (MQ=255)                                              gCGAGTGAAATGTTCTTAAATGTTTTTGACTGCTATTCAGTACAAGTTGTTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATT‑CAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCg                                                                                <  2:329039/140‑1 (MQ=255)                                                 aGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCggg                                                                              <  1:448606/140‑1 (MQ=255)                                                  gTGAAATGTTCTTCAGTGATTTCGACTGCGAATCAATTCAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGc                                                                             <  1:262099/140‑1 (MQ=255)                                                  gTGAAATGTTCTTCAATTATTTAGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGc                                                                             <  2:292189/140‑1 (MQ=255)                                                   tGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGGTTCCAGTAATAATAAGACGATACCTCAATTAAAGGCGTTAGGCGGGCa                                                                            >  2:247428/1‑140 (MQ=255)                                                               cAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTc                                                                >  2:716061/1‑140 (MQ=255)                                                                 aTGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCaa                                                              <  1:709389/140‑1 (MQ=255)                                                                     ttttGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGc                                                          <  1:647194/140‑1 (MQ=255)                                                                              cTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTg                                                 >  2:115897/1‑140 (MQ=255)                                                                                aaTCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGAc                                               <  1:551949/140‑1 (MQ=255)                                                                                    aaTACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTg                                           <  1:60217/140‑1 (MQ=255)                                                                                      tACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTa                                         >  2:496174/1‑140 (MQ=255)                                                                                           gTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAAtttt                                    >  2:658236/1‑140 (MQ=255)                                                                                              aTTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCa                                 <  2:504104/140‑1 (MQ=255)                                                                                               ttttCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  2:447152/140‑1 (MQ=255)                                                                                               ttttCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  1:496174/140‑1 (MQ=255)                                                                                               ttttCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  2:62118/140‑1 (MQ=255)                                                                                               ttttCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  1:88567/140‑1 (MQ=255)                                                                                               ttttCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  1:716061/140‑1 (MQ=255)                                                                                               ttttCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  1:708747/140‑1 (MQ=255)                                                                                               ttttCTCTGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  2:413271/140‑1 (MQ=255)                                                                                               ttttCTCTGGCCGTAAATATGAAAAAGCAGGCGGCGCGGTGTTCCGGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  2:143670/140‑1 (MQ=255)                                                                                                    tctGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCATCTCATAGGTTCAACTGACAGTCCCTAACGCCGTAATTTTTCACCGCta                           >  2:208419/1‑140 (MQ=255)                                                                                                    tctGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                <  1:140319/135‑1 (MQ=255)                                                                                                    tctGTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCAc                                >  2:140319/1‑135 (MQ=255)                                                                                                       gTCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATaa                        >  2:659476/1‑140 (MQ=255)                                                                                                        tCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATaaa                       <  1:735281/140‑1 (MQ=255)                                                                                                        tCCGTAAATATTAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACAGCCAGTCCCTGACGGTGTAATGTTTCACCGCTATaaa                       >  1:196568/1‑140 (MQ=255)                                                                                                                  ttAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCAcc               >  2:705191/1‑138 (MQ=255)                                                                                                                  ttAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCAcc               <  1:705191/138‑1 (MQ=255)                                                                                                                  ttAAAAAACAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCACCCa             >  2:585297/1‑140 (MQ=255)                                                                                                                        aaCAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCACCCAGAGTTg       >  2:491928/1‑140 (MQ=255)                                                                                                                          cAGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCACCCAGAGTTGAt     <  1:491928/140‑1 (MQ=255)                                                                                                                           aGGCGGCGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATCCCTGAATTAAAGGCGTTAGGCGGGCATCTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCACCCAGAGTTGATc    <  2:684624/140‑1 (MQ=255)                                                                                                                            ggcggcGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCACCCAGAGTTGAt     <  2:38902/138‑1 (MQ=255)                                                                                                                            ggcggcGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCACCCAGAGTTGAt     >  1:38902/1‑138 (MQ=255)                                                                                                                             gcggcgcggtTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTGTAAACCTGCGCCCAGAGTTGATCgg  >  1:303074/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                        GCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTTTCACCGCTATAAACCTGCACCCAGAGTTGATCGG  >  NC_000913/4069384‑4069647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑