Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,105,864	C→T	V15V (GTC→GTT)	cpxP →	periplasmic protein CpxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,105,864	0	C	T	100.0%	125.0 / NA	38	V15V (GTC→GTT)	cpxP	periplasmic protein CpxP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/22);  total (16/22)

TACGTTGTTTTACACCCCCTGACGCATGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA  >  NC_000913/4105728‑4105994                                                                                                                                         |                                                                                                                                   tACGTTGTTTTACACCCCCTGACGCATGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGcagttag                                                                                                                                  >  2:169459/1‑139 (MQ=255)   cGTTGTTTTACACCCTCTGACGCATCTTTTCAGCCTGTATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTc                                                                                                                               <  1:227834/140‑1 (MQ=255)       gttTTACACCCCCTGACGCATGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTa                                                                                                                           >  2:127493/1‑140 (MQ=255)            acacCCCCTGACGCATGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCa                                                                                                                      <  2:26556/140‑1 (MQ=255)                ccccTGACGCATGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACgct                                                                                                                  >  2:593611/1‑140 (MQ=255)                     gACGCATGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGa                                                                                                             >  2:603155/1‑140 (MQ=255)                        gCATGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGt                                                                                                          <  2:604167/140‑1 (MQ=255)                           tGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCgg                                                                                                       >  2:585694/1‑140 (MQ=255)                             tttGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGtt                                                                                                     <  1:497409/140‑1 (MQ=255)                                gCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTGATTTAGGCACGCTGCTGAAGTCGGTTCAg                                                                                                  >  1:273300/1‑140 (MQ=255)                                 cAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCCTTAAGCCACGCTGATGAAGTCGGTTCAgg                                                                                                 >  1:277593/1‑140 (MQ=255)                                  aGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGc                                                                                                >  1:75961/1‑140 (MQ=255)                                     cTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGAt                                                                                             <  1:593611/140‑1 (MQ=255)                                      tGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATa                                                                                            >  2:684633/1‑140 (MQ=255)                                        aaTCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAAc                                                                                          >  2:470973/1‑140 (MQ=255)                                        aaTCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAAc                                                                                          <  1:410904/140‑1 (MQ=255)                                                aCTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATcc                                                                                  <  2:440476/140‑1 (MQ=255)                                                  tctctATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTACATTAAGCGACGGTGCGGAAGTCGGGTCAGGCGATAACTGGCATCCgg                                                                                >  2:218016/1‑140 (MQ=255)                                                      tATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTga                                                                            >  2:419636/1‑140 (MQ=255)                                                      tATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTga                                                                            <  1:721325/140‑1 (MQ=255)                                                        tCGTTGAATCGCGACAGAAAGATTTTGGGCGCAAATGTTGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTgaag                                                                          <  1:278228/140‑1 (MQ=255)                                                        tCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTgaag                                                                          <  2:576730/140‑1 (MQ=255)                                                         cGTTGAATCGCGACAGACAGATGTTGGGAGCAAATTATGCGCATAGTTTCCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTgaaga                                                                         <  2:277593/140‑1 (MQ=255)                                                              aaTCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTga                                                                            <  1:397538/132‑1 (MQ=255)                                                              aaTCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTga                                                                            >  2:397538/1‑132 (MQ=255)                                                                     aCAGAAAGATTTTGGGAGCAAAAGATGCGCACAGTCACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcg                                                             <  2:273300/140‑1 (MQ=255)                                                                       aGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgca                                                           <  1:419636/140‑1 (MQ=255)                                                                            gATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGTGCAGTACg                                                      <  1:603155/140‑1 (MQ=255)                                                                            gATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACg                                                      <  1:650291/140‑1 (MQ=255)                                                                                       cAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGc                                                     >  2:165213/1‑130 (MQ=255)                                                                                       cAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGc                                                     <  1:165213/130‑1 (MQ=255)                                                                                                 gcATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGc                                 <  1:526863/140‑1 (MQ=255)                                                                                                           cGCTGCCGTCATGGCCTCAACGATGCCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTaa                       <  1:218016/140‑1 (MQ=255)                                                                                                               gCCGTCATGGCCTCAACGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                   >  2:89670/1‑140 (MQ=255)                                                                                                                              aCGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagca    <  1:684633/140‑1 (MQ=255)                                                                                                                              aCGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagca    <  1:470973/140‑1 (MQ=255)                                                                                                                              aCGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagca    <  1:43248/140‑1 (MQ=255)                                                                                                                               cGCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGCCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagcag   >  2:436947/1‑140 (MQ=255)                                                                                                                                gCTGGCAGTTAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa  >  1:443764/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   TACGTTGTTTTACACCCCCTGACGCATGTTTGCAGCCTGAATCGTAAACTCTCTATCGTTGAATCGCGACAGAAAGATTTTGGGAGCAAATGATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA  >  NC_000913/4105728‑4105994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑