Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,155,941 C→T A314V (GCG→GTG)  argC → N‑acetylglutamylphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,155,9410CT97.0% 102.7 / NA 33A314V (GCG→GTG) argCN‑acetylglutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (15/17);  total (15/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGG  >  NC_000913/4155804‑4156073
                                                                                                                                         |                                                                                                                                    
gCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGcggtgg                                                                                                                                    >  1:680570/1‑140 (MQ=255)
 cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggtggc                                                                                                                                   >  1:115303/1‑140 (MQ=255)
 cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggtggc                                                                                                                                   >  1:179232/1‑140 (MQ=255)
 cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCCGAAAAATGTCGTTGGGCTGCCATTTTACGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggtggc                                                                                                                                   >  1:396395/1‑140 (MQ=255)
 cTGGTGCGGCTGTATCACAAAGTCGTTCCCGCGCTGATAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggtggc                                                                                                                                   <  1:219445/140‑1 (MQ=255)
 cTGGGGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGGCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggtggc                                                                                                                                   <  2:14311/140‑1 (MQ=255)
         gCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCgg                                                                                                                           >  1:135195/1‑140 (MQ=255)
                 aCAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTgc                                                                                                                   <  2:388672/140‑1 (MQ=255)
                       gCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGTAAGACGCGGTGGAACAAACGGTACAGTGCGCCAat                                                                                                             >  2:299842/1‑140 (MQ=255)
                         gTTCCGGCGCTGAAAAATGGCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAatat                                                                                                           >  1:141218/1‑140 (MQ=255)
                          ttCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATAtt                                                                                                          <  2:121113/140‑1 (MQ=255)
                              ggCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGtt                                                                                                      <  2:135195/140‑1 (MQ=255)
                                 gcTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCg                                                                                                   >  1:423147/1‑140 (MQ=255)
                                 gcTGAAAAATGTCATTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCg                                                                                                   >  1:423151/1‑140 (MQ=255)
                                  cTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCgg                                                                                                  <  2:423147/140‑1 (MQ=255)
                                  cTGAAAAATGTCATTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCgg                                                                                                  <  2:423151/140‑1 (MQ=255)
                                           gTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGa                                                                                         <  2:377896/140‑1 (MQ=255)
                                               ttGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACg                                                                                     <  1:693049/140‑1 (MQ=255)
                                                 gggCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCa                                                                                   <  1:438655/140‑1 (MQ=255)
                                                 gggCTGCCATTTTGCGATATCGAGTGTGCAGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCa                                                                                   <  1:293713/140‑1 (MQ=255)
                                                    cTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGtc                                                                                >  2:105692/1‑140 (MQ=255)
                                                      gCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGtctc                                                                              <  2:141218/140‑1 (MQ=255)
                                                      gCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGtctc                                                                              >  2:621248/1‑140 (MQ=255)
                                                      gCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAACATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGtctc                                                                              <  1:40986/140‑1 (MQ=255)
                                                                gATATCGGGTTTGCCCTTCAGGGCGTGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAgg                                                                    <  1:299842/140‑1 (MQ=255)
                                                                    tCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTaa                                                                      <  1:734950/134‑1 (MQ=255)
                                                                    tCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTaa                                                                      >  2:734950/1‑134 (MQ=255)
                                                                              cGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCTGTCACTGATTTAAAGGTGCAATGATGAAt                                                      >  1:193187/1‑140 (MQ=255)
                                                                              cGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAAt                                                      <  1:105692/140‑1 (MQ=255)
                                                                                               tGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGAGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACt                                     <  1:200068/140‑1 (MQ=255)
                                                                                                  ttattGTGGCGACCGAACACAATTTACTGAATGGCCCGGCGGCACAAGTGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTggg                                  <  2:203554/140‑1 (MQ=255)
                                                                                                       gtgGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg                             >  2:411584/1‑140 (MQ=255)
                                                                                                       gtgGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg                             <  2:680570/140‑1 (MQ=255)
                                                                                                        tgGCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTGATTTAAGGGTGCGATGGTGAATCCAGTAATGATCAAACTGGGCGGCGt                            >  2:200068/1‑140 (MQ=255)
                                                                                                          gCGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTAc                          >  2:23991/1‑140 (MQ=255)
                                                                                                           cGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTAct                         >  2:410037/1‑140 (MQ=255)
                                                                                                           cGACCGAAGACAACTTACTGAAAGGCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTAct                         >  2:667739/1‑140 (MQ=255)
                                                                                                                 aaGACAACTTACTGAAAAGCGCAGTGGCACAAGCGATACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGa                   <  2:265072/140‑1 (MQ=255)
                                                                                                                                  ggCGCGGTGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTgg  >  1:627321/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                    
GCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGG  >  NC_000913/4155804‑4156073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑