Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,375	T→C	T287A (ACG→GCG)	lysC ←	aspartate kinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,375	0	T	C	100.0%	119.3 / NA	34	T287A (ACG→GCG)	lysC	aspartate kinase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/13);  total (21/13)

GATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTT  >  NC_000913/4232241‑4232509                                                                                                                                           |                                                                                                                                       gATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGcaccac                                                                                                                                   <  1:540611/140‑1 (MQ=255)   tGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCt                                                                                                                                 <  2:178676/140‑1 (MQ=255)        aaaaCTTCCGCGAGGAACCCGCTAGAATGCACCATCTTCCGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGcgcg                                                                                                                            <  2:203757/140‑1 (MQ=255)              tCCGCGAGGAAACCGGGAGAATTCACCATGTTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTc                                                                                                                      <  1:241756/140‑1 (MQ=255)                        aaCCGCGAGAATGCAGCTTATTCAGGCTGTGTAAACTGACCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAg                                                                                                            <  2:289574/140‑1 (MQ=255)                        aaCCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAg                                                                                                            <  1:667941/140‑1 (MQ=255)                               agaATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACaa                                                                                                     >  1:132879/1‑140 (MQ=255)                                gaATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACaaa                                                                                                    <  2:170507/140‑1 (MQ=255)                                             tCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCg                                                                                       >  2:447464/1‑140 (MQ=255)                                               aGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCt                                                                                     >  2:662977/1‑140 (MQ=255)                                                  cTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCg                                                                                  >  2:59467/1‑140 (MQ=255)                                                     tgCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGAc                                                                                                 <  2:699223/122‑1 (MQ=255)                                                     tgCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGAc                                                                                                 >  1:699223/1‑122 (MQ=255)                                                     tgCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTAc                                                                               >  2:54172/1‑140 (MQ=255)                                                     tgCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTAc                                                                               >  1:440533/1‑140 (MQ=255)                                                               gCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCggg                                                                         >  1:90661/1‑140 (MQ=255)                                                               gCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGCAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGc                                                                     >  2:13277/1‑140 (MQ=255)                                                                     tCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTAGAGCCGACCAAGACCGAGATATCGCTGCGTCCTGCGGGTAGCAACGtt                                                               >  2:266014/1‑140 (MQ=255)                                                                        gATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGcc                                                            >  2:522195/1‑140 (MQ=255)                                                                             cgacgaAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATg                                                       >  2:634575/1‑140 (MQ=255)                                                                             cgacgaAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATg                                                       >  2:622268/1‑140 (MQ=255)                                                                             cgacgaAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATg                                                       >  2:610149/1‑140 (MQ=255)                                                                             cgacgaAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATg                                                       >  1:471695/1‑140 (MQ=255)                                                                             cgacgaAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATg                                                       <  1:59467/140‑1 (MQ=255)                                                                              gacgaAGCGCCAGAGCGCG‑‑‑‑GAATAGAGGCCGATTTTCTGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGc                                                      <  1:356711/140‑1 (MQ=255)                                                                               acgaAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCa                                                     >  2:78697/1‑140 (MQ=255)                                                                               acgaAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCa                                                     >  2:152517/1‑140 (MQ=255)                                                                                     cgcCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTCCGTACTGTGGGCAGAAACGTTCCCGGATGCAGTACtt                                               >  1:247242/1‑140 (MQ=255)                                                                                      gcCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACttt                                              <  1:78697/140‑1 (MQ=255)                                                                                              gcg‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCaa                                      <  1:662977/140‑1 (MQ=255)                                                                                                           cggcggATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCa                             >  2:28396/1‑140 (MQ=255)                                                                                                           cggcggATTTTCAGTTTTATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCa                             <  2:132879/140‑1 (MQ=255)                                                                                                                         ttttATTGCACACCAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCg               <  2:90661/140‑1 (MQ=255)                                                                                                                                      cAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCGAAAGTTGGCATGTCGGCCGCTGCGGCAAACGCGAttt  >  1:202925/1‑140 (MQ=255)                                                                                                                                      cAGCGCACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGAttt  >  1:57756/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       GATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCG‑‑‑‑GAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGACCGGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTT  >  NC_000913/4232241‑4232509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑