Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,401,719	A→G	I350V (ATA→GTA)	hflX →	ribosome rescue factor HflX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,401,719	0	A	G	100.0%	149.6 / NA	43	I350V (ATA→GTA)	hflX	ribosome rescue factor HflX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/22);  total (21/22)

ATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGG  >  NC_000913/4401599‑4401854                                                                                                                         |                                                                                                                                        aTCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGc                                                                                                                      >  2:656414/1‑140 (MQ=255)   cccAACCCTGCTGGTGAGGAACGAGATCGATCTGCGGGAGGATTTCGACCCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCtt                                                                                                                    <  2:270382/140‑1 (MQ=255)      aaCCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGa                                                                                                                 >  1:647382/1‑140 (MQ=255)           tgctgGTGATGAACAAGCTCGATATGCTGGATGATTTCGGACCGCGTACTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAg                                                                                                            <  2:221309/140‑1 (MQ=255)             ctgGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGGACAGACCGCAGCGGGGGTACCACAGATTTTCCAGGCTTTGACGGAGCg                                                                                                          >  2:270755/1‑140 (MQ=255)             ctgGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCg                                                                                                          >  2:404401/1‑140 (MQ=255)             ctgGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAACGGGGGTCCCATAGATTTTTCTGGCTTTGACGGAGCg                                                                                                          >  1:344241/1‑140 (MQ=255)                  gatgaACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACg                                                                                                               <  2:621834/130‑1 (MQ=255)                  gatgaACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACg                                                                                                               >  1:621834/1‑130 (MQ=255)                  gatgaACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCttt                                                                                                     >  1:534246/1‑140 (MQ=255)                       aCAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGc                                                                                                >  1:111705/1‑140 (MQ=255)                          aGATCGATTTGCTGGAGGATTTGGAACCGCATACTGATCAGGACGAAGGGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAg                                                                                             <  2:344241/140‑1 (MQ=255)                          aGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAg                                                                                             >  2:654044/1‑140 (MQ=255)                          aGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAg                                                                                             >  1:423669/1‑140 (MQ=255)                             tCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTg                                                                                          <  2:124756/140‑1 (MQ=255)                              ggATATGCT‑GAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgc                                                                                        <  1:470418/139‑1 (MQ=255)                               gATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgc                                                                                        <  2:370760/140‑1 (MQ=255)                               gATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgc                                                                                        <  2:4583/140‑1 (MQ=255)                               gATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgc                                                                                        <  1:654044/140‑1 (MQ=255)                                     cTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgcgc                                                                                      <  2:509574/136‑1 (MQ=255)                                     cTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgcgc                                                                                      <  2:87230/136‑1 (MQ=255)                                     cTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgcgc                                                                                      >  1:87230/1‑136 (MQ=255)                                     cTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgcgc                                                                                      >  1:509574/1‑136 (MQ=255)                                       ggAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATa                                                                                >  1:107336/1‑140 (MQ=255)                                        gAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCATTTTCAGGTTTTAACGGAGCGGCTTACCGGTGAGGTGGCGCAGCATAc                                                                               >  2:281458/1‑140 (MQ=255)                                                  aaCCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTg                                                                     >  1:177619/1‑140 (MQ=255)                                                      gcgTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCTTCTGCCAc                                                                 <  2:423669/140‑1 (MQ=255)                                                                 gggACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCTCAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAggg                                                      >  1:424171/1‑140 (MQ=255)                                                                 gggACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAggg                                                      <  1:652274/140‑1 (MQ=255)                                                                 gggACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAggg                                                      <  2:534246/140‑1 (MQ=255)                                                                 gggACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAggg                                                      <  2:107336/140‑1 (MQ=255)                                                                                 accgaaccgTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGtt                                      >  1:524015/1‑140 (MQ=255)                                                                                 accgaaccgTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTACGTCTGCAACCGCAAGAAGGGCGTCTGAGAAGTCGtt                                      >  1:329653/1‑140 (MQ=255)                                                                                 accgaaccgTGTCTGGCTTTCCGCACAGACCGGAGCGGGCGTACCACACCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGtt                                      <  2:222705/140‑1 (MQ=255)                                                                                      accgTGTCTGGCTTTACGCACAAACAGGACCGGGGGTCCCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTAt                                 <  1:270755/140‑1 (MQ=255)                                                                                                        cacaGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGaa               >  2:517654/1‑140 (MQ=255)                                                                                                           aGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGTGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGaaaaa            >  1:73042/1‑140 (MQ=255)                                                                                                           aGACCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCAGAGAAGTCGTTTTTCTCAGCTGCAGGCAATAGaaaaa            >  1:314985/1‑140 (MQ=255)                                                                                                             aCCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAaga          <  1:656414/140‑1 (MQ=255)                                                                                                             aCCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAaga          <  1:404401/140‑1 (MQ=255)                                                                                                             aCCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAaga          <  2:603014/140‑1 (MQ=255)                                                                                                             aCCGGAGCGGGGGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAaga          <  2:647382/140‑1 (MQ=255)                                                                                                                     ggggGTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtggatgg  <  1:517654/140‑1 (MQ=255)                                                                                                                         |                                                                                                                                        ATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGG  >  NC_000913/4401599‑4401854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑