Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	555,650	A→G	D347G (GAC→GGC)	cysS →	cysteine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	555,650	0	A	G	100.0%	139.3 / NA	40	D347G (GAC→GGC)	cysS	cysteine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/23);  total (17/23)

AGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGACGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGCAGAAGATATGGCAGCGGCGAATGCAATGGCATCTCACCTGCGTAAACTTTCCGCTGTATTGGG  >  NC_000913/555512‑555785                                                                                                                                           |                                                                                                                                        aGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGgac                                                                                                                                        >  1:733468/1‑140 (MQ=255)    tGAACTACAGCGAAGAGTACCTTAAGCAGGCGCGTGCGGAGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGggcgac                                                                                                                                     <  2:273893/140‑1 (MQ=255)         tACAGCGAAGAGAACCTGAAGCCGGTGCGGGCGGCGCCGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGAttt                                                                                                                                <  1:361072/140‑1 (MQ=255)             gCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAac                                                                                                                            >  1:732088/1‑140 (MQ=255)              cGAAGAGAACCTGAAGCAGGCGTGTGCGGCGCTGGAGGGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAaca                                                                                                                           <  1:316477/140‑1 (MQ=255)              cGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAaca                                                                                                                           >  2:607197/1‑140 (MQ=255)                aaGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACAcc                                                                                                                         >  2:656810/1‑140 (MQ=255)                   agaACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCg                                                                                                                      <  1:607197/140‑1 (MQ=255)                   agaACCTGAAGCAGGCGCGTGCGGCACTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCg                                                                                                                      <  1:257591/140‑1 (MQ=255)                   agaACCTGAAGCAGGAGCGTGCTGCACTGTAGCGTCTATACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCg                                                                                                                      <  1:218363/140‑1 (MQ=255)                        cTGAAGCAGGCGCGTGCGGCGCTGGTGCGCCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGc                                                                                                                 <  1:229969/140‑1 (MQ=255)                        cTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGc                                                                                                                 <  1:109306/140‑1 (MQ=255)                        cTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGc                                                                                                                 <  1:643048/140‑1 (MQ=255)                          gAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCt                                                                                                               <  1:622709/140‑1 (MQ=255)                             gCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTAtt                                                                                                            >  1:536323/1‑140 (MQ=255)                               aGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTcc                                                                                                          >  1:468224/1‑140 (MQ=255)                                  ggcgTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTa                                                                                                       <  2:690902/139‑1 (MQ=255)                                  cgcgTGCGGCGCTGGGGCGTCTGTACACTGCGCTGCGTGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTa                                                                                                       <  1:243408/140‑1 (MQ=255)                                  cgcgTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTa                                                                                                       <  1:20397/140‑1 (MQ=255)                                  cgcgTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTa                                                                                                       <  1:656810/140‑1 (MQ=255)                                    cgTGCGGCGCTGGAGCGCCTCTCCACTGCGCTGCGCGGCCCAGATAAACCCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACt                                                                                                     <  2:194471/140‑1 (MQ=255)                                           cgcTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGat                                                                                              <  2:579710/140‑1 (MQ=255)                                                  gCGTCTCTACACTGCGTAGCGCGGCACAGATCACACCGTCGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGTGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGgcgc                                                                                       <  2:334064/140‑1 (MQ=255)                                                                cgctgcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGTGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGt                                                                         >  1:46794/1‑140 (MQ=255)                                                                cgctgcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTTTTCCGTAGTGTGTGGTATGGCGCGTGAAGTAAACCGt                                                                         >  1:297377/1‑140 (MQ=255)                                                                cgctgcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGgcgc                                                                                       <  2:452837/126‑1 (MQ=255)                                                                cgctgcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGgcgc                                                                                       >  1:452837/1‑126 (MQ=255)                                                                cgctgcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGt                                                                         >  1:704284/1‑140 (MQ=255)                                                                cgctgcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGt                                                                         >  1:46224/1‑140 (MQ=255)                                                                cgctgcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGt                                                                         >  1:370166/1‑140 (MQ=255)                                                                   tgcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTg                                                                      >  2:28389/1‑140 (MQ=255)                                                                    gcgcGGCACAGATAAAATCGTTTCGCCTGCCGGTGACGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGa                                                                     <  2:297377/140‑1 (MQ=255)                                                                    gcgcGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGa                                                                     <  2:107032/140‑1 (MQ=255)                                                                     cgcgGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGaa                                                                    >  1:72395/1‑140 (MQ=255)                                                                         gCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGCa                                                                <  1:28389/140‑1 (MQ=255)                                                                                        ttGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGc                                                                 <  1:714787/124‑1 (MQ=255)                                                                                        ttGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGc                                                                 <  1:592694/124‑1 (MQ=255)                                                                                        ttGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGc                                                                 >  2:592694/1‑124 (MQ=255)                                                                                        ttGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGc                                                                 >  2:714787/1‑124 (MQ=255)                                                                                                gCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGCAGAAGATATGGCAGCGGCGAATGc                                         >  2:596064/1‑140 (MQ=255)                                                                                                                                   aGCGATGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGCAGAAGATATGGCAGCGGCGAATGCAATGGCATCTCACCTGCGTAAACTTTCCGCTGTAt      <  2:46794/140‑1 (MQ=255)                                                                                                                                       aTGGGCGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGCAGAAGATATGGCAGCGGCGAATGCAATGGCATCTCACCTGCGTAAACTTTCCGCTGTATTggg  >  1:91301/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        AGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGACGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCGTGAAGTAAACCGTCTGAAAGCAGAAGATATGGCAGCGGCGAATGCAATGGCATCTCACCTGCGTAAACTTTCCGCTGTATTGGG  >  NC_000913/555512‑555785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑