Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,448,792	T→C	V34A (GTG→GCG)	chpB →	endoribonuclease toxin ChpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,448,792	0	T	C	100.0%	225.8 / NA	62	V34A (GTG→GCG)	chpB	endoribonuclease toxin ChpB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/31);  total (31/31)

TGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCG  >  NC_000913/4448656‑4448920                                                                                                                                         |                                                                                                                                 tGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCtgcgcttgcgct                                                                                                                               <  2:623026/140‑1 (MQ=255)   tCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGctct                                                                                                                             <  2:676630/140‑1 (MQ=255)   tCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGctct                                                                                                                             <  2:451165/140‑1 (MQ=255)         gTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTc                                                                                                                       <  2:105237/140‑1 (MQ=255)         gTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTc                                                                                                                       >  1:75338/1‑140 (MQ=255)         gTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTc                                                                                                                       >  1:528216/1‑140 (MQ=255)         gTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCAGCCATGAAAAGCGAGGGGCTGGTCGTCCTGCGCTTGCGCTCTCCGTTc                                                                                                                       >  2:271351/1‑140 (MQ=255)          tAAATCCACCCCTGCTGGTGACTAACTATGGTAAAGACAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCa                                                                                                                      <  1:339278/140‑1 (MQ=255)           aaaTCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCaa                                                                                                                     >  2:63123/1‑140 (MQ=255)             aTCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGc                                                                                                                   >  1:80528/1‑140 (MQ=255)             aTCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGc                                                                                                                   <  1:672535/140‑1 (MQ=255)              tCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGcc                                                                                                                  >  2:151682/1‑140 (MQ=255)              tCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGcc                                                                                                                  <  1:367056/140‑1 (MQ=255)               ccACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCt                                                                                                                 <  1:26543/140‑1 (MQ=255)                    ccTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAAt                                                                                                            >  2:89823/1‑140 (MQ=255)                        cGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAAc                                                                                                        >  1:649338/1‑140 (MQ=255)                             gACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGGGCTTGCGATCTACGATCAAGCGTTTAATCAACTGGGa                                                                                                   >  1:345735/1‑140 (MQ=255)                                          aaaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCACCGTTTATGCCTTTAAGCAACTGGGAATGAGGCtggtgg                                                                                      >  2:361063/1‑140 (MQ=255)                                           aaGAAAAGTGAATTTTAACGGGTAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                                                                     <  1:361063/140‑1 (MQ=255)                                           aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                                                                     <  1:151682/140‑1 (MQ=255)                                           aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                                                                     <  2:185069/140‑1 (MQ=255)                                           aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                                                                     <  1:631151/140‑1 (MQ=255)                                           aaGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                                                                     <  2:75338/140‑1 (MQ=255)                                           aaGAAAAGTGAATTTCAACGGGCAGACATAGTGCTGGGTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGc                                                                                     <  1:271351/140‑1 (MQ=255)                                              aaaaGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGcccc                                                                                  >  2:24147/1‑140 (MQ=255)                                                 aGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCAt                                                                               >  2:419653/1‑140 (MQ=255)                                                     aaTTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACg                                                                           >  1:732230/1‑140 (MQ=255)                                                              ggggAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGa                                                                  <  1:426415/140‑1 (MQ=255)                                                                          tgCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGt                                                      >  1:686954/1‑140 (MQ=255)                                                                          tgCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGt                                                      >  2:683315/1‑140 (MQ=255)                                                                           gCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGtt                                                     <  1:419653/140‑1 (MQ=255)                                                                             tGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTAt                                                   <  2:80528/140‑1 (MQ=255)                                                                               gTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGc                                                 <  1:683315/140‑1 (MQ=255)                                                                               gTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGc                                                 >  1:618131/1‑140 (MQ=255)                                                                                ttGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCGCCAATACGCAGGGCGGAGATTTTGCCCGTTATGcc                                                >  2:214073/1‑140 (MQ=255)                                                                                  ggCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCGCCATTACCCAGAGCTGAAATTATGCCCGTTATGCCgg                                              >  1:336482/1‑140 (MQ=255)                                                                                   gCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGa                                             <  1:63123/140‑1 (MQ=255)                                                                                    cTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGAt                                            >  2:572863/1‑140 (MQ=255)                                                                                         aTCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTTGCCCCCATGACGTAGGGCGGAAATTATGCCTGTTATGCCGGATTTAGc                                       >  2:231252/1‑140 (MQ=255)                                                                                          tCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCg                                      <  2:173789/140‑1 (MQ=255)                                                                                          tCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCg                                      <  2:373980/140‑1 (MQ=255)                                                                                          tCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCg                                      <  2:528216/140‑1 (MQ=255)                                                                                             aGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCg                                      <  1:534398/137‑1 (MQ=255)                                                                                             aGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCg                                      >  2:534398/1‑137 (MQ=255)                                                                                              gCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTcc                                  >  1:463095/1‑140 (MQ=255)                                                                                               cAAGCGGCGATGAACAAAAAGGTGCTGGTCGTCTTGCGCGTGCGCTCTGCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCt                                 <  2:345735/140‑1 (MQ=255)                                                                                               cAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCt                                 <  1:89823/140‑1 (MQ=255)                                                                                               cAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCt                                 <  2:463095/140‑1 (MQ=255)                                                                                                        aTGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGc                        >  2:445491/1‑140 (MQ=255)                                                                                                         tGAACAGCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTTTGCGGGGTTTAGCTTTCCTTTACATTGCg                       >  1:267504/1‑140 (MQ=255)                                                                                                               gCAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGaagaag                 <  2:649338/140‑1 (MQ=255)                                                                                                                cAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAgg                >  2:606197/1‑140 (MQ=255)                                                                                                                cAAGGTGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAgg                >  2:591451/1‑140 (MQ=255)                                                                                                                     tGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGAtg           >  2:35125/1‑140 (MQ=255)                                                                                                                     tGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGAtg           >  1:33055/1‑140 (MQ=255)                                                                                                                     tGCTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGAtg           >  1:500020/1‑140 (MQ=255)                                                                                                                       cTGGTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGAtgtg         <  2:407347/140‑1 (MQ=255)                                                                                                                        tGGTCGACCTGCGCTTGCGCTCTCCGTTCACGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGc        >  2:705340/1‑140 (MQ=255)                                                                                                                         ggTCGACCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCa       <  1:35125/140‑1 (MQ=255)                                                                                                                          gTCGACCCGCGCTTGCGCTATCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCAc      >  2:422552/1‑140 (MQ=255)                                                                                                                              aCCTGCGCTTGCGCTGTCCGTTTAACCCTCTAATCAATTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCg  <  1:231252/140‑1 (MQ=255)                                                                                                                              aCCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCg  <  2:500020/140‑1 (MQ=255)                                                                                                                              aCCTGCGCTTGCGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCg  <  1:445491/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 TGTCTGGGGTAAATCCACCCCTGCGGGTGACGAAATATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCG  >  NC_000913/4448656‑4448920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑