Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,571,048	C→T	G176D (GGC→GAC)	yjiR ←	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,571,048	0	C	T	100.0%	128.1 / NA	39	G176D (GGC→GAC)	yjiR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/23);  total (16/23)

GTTGGGATTTCAATCACTTTCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCAC  >  NC_000913/4570913‑4571184                                                                                                                                        |                                                                                                                                         gTTGGGATTTCAATCACTTTCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCt                                                                                                                                      >  1:527497/1‑140 (MQ=255)   tGGGATTTCAATCACTTTCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGAATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTgg                                                                                                                                    <  1:591297/140‑1 (MQ=255)     ggATTTCAATCACTTTCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGtg                                                                                                                                  <  1:127984/140‑1 (MQ=255)           cAATCACTTTCACGCCCATGCCCCGTAGCTTCTGCATTGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGtgatgatg                                                                                                                            <  1:238488/140‑1 (MQ=255)            aaTCACTTTCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGtga                                                                                                                                 >  1:38479/1‑134 (MQ=255)            aaTCACTTTCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGtga                                                                                                                                 <  2:38479/134‑1 (MQ=255)                  tttCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCAt                                                                                                                     <  2:166276/140‑1 (MQ=255)                  tttCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCAt                                                                                                                     <  2:66187/140‑1 (MQ=255)                            aTGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGgagat                                                                                                           >  1:735369/1‑139 (MQ=255)                                  cgcAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGGTGTCGTCGGCGGTGaccacccc                                                                                                     >  2:215313/1‑138 (MQ=255)                                  cgcAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGaccacccc                                                                                                     >  2:374991/1‑138 (MQ=255)                                      gcaTCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCg                                                                                                 <  2:478363/140‑1 (MQ=255)                                      gcaTCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCg                                                                                                 <  2:545660/140‑1 (MQ=255)                                               tCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGAACCCCCCGCCGCCGTCGAGc                                                                                        >  2:379980/1‑140 (MQ=255)                                                  aaCCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGTCGGGGAACACCCAGCCCCTGTCGGGGATc                                                                                     >  1:312685/1‑140 (MQ=255)                                                     cGTAATAACAGGGGGATTCGACTGCGACAATTTCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAgg                                                                                  <  2:275765/140‑1 (MQ=255)                                                     cGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAgg                                                                                  <  2:506379/140‑1 (MQ=255)                                                               gggggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCt                                                                        >  1:633403/1‑140 (MQ=255)                                                               gggggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCCAGCCGCCGTCGGGCATCAGGCGGGGCATCt                                                                        >  2:601469/1‑140 (MQ=255)                                                               gggggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGGGAGCACCCAGCCGCCGGCGCGCCGCAGGCGGGCAAACt                                                                        >  2:160860/1‑140 (MQ=255)                                                                 gggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGc                                                                      <  2:633403/140‑1 (MQ=255)                                                                 gggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGc                                                                      <  1:558580/140‑1 (MQ=255)                                                                 gggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCCAGCCGCTGTCGGGCATCAGGCGGGCCATCTGc                                                                      >  1:68245/1‑140 (MQ=255)                                                                 gggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCCAGCCGCCGTCGAGCATCAAGCGGGccaaccgc                                                                      >  2:563622/1‑133 (MQ=255)                                                                 gggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCCAGCCCCTGTCGGGCATCAAGCGGGCAAACTGc                                                                      >  1:706224/1‑140 (MQ=255)                                                                  ggATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCCAGCCGCTGTCGAGCATCAGGCGGGCAATCTGct                                                                     >  1:622207/1‑140 (MQ=255)                                                                     ttCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCtctc                                                                  <  1:600299/140‑1 (MQ=255)                                                                                    atCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGAc                                                   <  1:379980/140‑1 (MQ=255)                                                                                           ggTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTaa                                            <  1:581979/140‑1 (MQ=255)                                                                                           ggTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTaa                                            <  1:160860/140‑1 (MQ=255)                                                                                           ggTTTACACACTGCCATCAACGGCAGCGACATCTACTTAAGGCAGTCGGTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTaa                                            <  2:312685/140‑1 (MQ=255)                                                                                                  acacTGCCATTAACGCCAGCGATATCGAGTTATGGCATTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCa                                     <  1:215313/140‑1 (MQ=255)                                                                                                       gCCATTAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTTGGGCATCACGCGGGCAATCTGGTCTCGCCATAATCCGTGGCCCGCCCACAAATCATAAcc                                >  2:649288/1‑140 (MQ=255)                                                                                                           ttAACGCCAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCgaga                            <  1:671841/140‑1 (MQ=255)                                                                                                                  cAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGttt                     <  2:735369/140‑1 (MQ=255)                                                                                                                  cAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGttt                     <  2:527497/140‑1 (MQ=255)                                                                                                                  cAGCGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGttt                     <  1:601469/140‑1 (MQ=255)                                                                                                                     cGACATCGAGTTATGGCAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCCAGCCGCCGTCGGGCATCAAGCGGGGAAACTGCTCTCGCCATACTCGCTGACCCGCCAACAAAACATAACCCAGAAAGGGTTGcc                  >  2:505463/1‑139 (MQ=255)                                                                                                                                     cAGTCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGcaccac  <  2:118113/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GTTGGGATTTCAATCACTTTCACGCCCATGCCGCGCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGGTTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCAC  >  NC_000913/4570913‑4571184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑