Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,666	T→C	V324A (GTT→GCT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,666	0	T	C	100.0%	124.7 / NA	36	V324A (GTT→GCT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/19);  total (17/19)

AAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGA  >  NC_000913/4637531‑4637801                                                                                                                                        |                                                                                                                                        aaaTTTTACGCGAAGATCCGCCCCCTGAACTGGTGGCACGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTg                                                                                                                                     <  1:209689/140‑1 (MQ=255)  aaTTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGa                                                                                                                                    <  1:118999/140‑1 (MQ=255)  aaTTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGa                                                                                                                                    <  1:507357/140‑1 (MQ=255)   aTTTTACGGGAAGGTCTGCCGCTGGAAGTGGTGGCGCGTGTTACTGACCACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGAc                                                                                                                                   <  2:351174/140‑1 (MQ=255)   aTTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGAc                                                                                                                                   >  2:389178/1‑140 (MQ=255)      ttACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGActgc                                                                                                                                <  2:552574/140‑1 (MQ=255)                 tCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTgg                                                                                                                     <  2:682992/140‑1 (MQ=255)                               tggtggCTCGTTTTACTGACAATATTCTGACGCAACATGGGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTccgc                                                                                                       <  2:318652/140‑1 (MQ=255)                                    gCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGt                                                                                                  <  2:552561/140‑1 (MQ=255)                                                gACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAgcgcg                                                                                      <  2:721021/140‑1 (MQ=255)                                                gACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAgcgcg                                                                                      <  1:12986/140‑1 (MQ=255)                                                gACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAgcgcg                                                                                      <  1:384922/140‑1 (MQ=255)                                                gACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCG‑Agcgcgc                                                                                     >  2:402563/1‑140 (MQ=255)                                                  caacaTTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCa                                                                                    >  2:442942/1‑140 (MQ=255)                                                    acaTTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAgcgcg                                                                                      <  1:572581/136‑1 (MQ=255)                                                    acaTTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAgcgcg                                                                                      >  2:572581/1‑136 (MQ=255)                                                               cAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATAATTACGCAGCGTCAGCTAAGCTCGCACCGTGCAGTTGGc                                                                       >  2:234925/1‑140 (MQ=255)                                                                aaaaTGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCa                                                                      <  1:389178/140‑1 (MQ=255)                                                                             gCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCa                                                         <  2:411965/140‑1 (MQ=255)                                                                              cAGGCATTGGTAGAACCGTTACTACCCCATGCAAGACCGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCAc                                                        <  1:234925/140‑1 (MQ=255)                                                                              cAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCTCCGCGTCACCGACGCGGGCACCGTGGAGTTGGCAGAAAAAAAAATCAc                                                        >  2:284810/1‑140 (MQ=255)                                                                               aGGCATTGGTAGAAACGCTACTTCGCCAGGCCAGAATGGAGAATCGTCCGGAAGTCGCACTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCAct                                                       <  2:220725/140‑1 (MQ=255)                                                                                 gCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCActct                                                     >  2:33416/1‑140 (MQ=255)                                                                                 gCATTGGTAGAAACGTTACTACCCCGGGCAAGACTGGCGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCActct                                                     <  1:284810/140‑1 (MQ=255)                                                                                       gTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGt                                               >  2:135277/1‑140 (MQ=255)                                                                                               gTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGACATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCa                                       >  1:180886/1‑140 (MQ=255)                                                                                                 tactacGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCAcc                                     >  1:610769/1‑140 (MQ=255)                                                                                                        ccAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGTAGTTGGCATAAAAAAAAATCACTCTACATGATACGCCCACCGAGGTTa                              >  2:202756/1‑140 (MQ=255)                                                                                                                     gagaATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGa                 <  2:391918/140‑1 (MQ=255)                                                                                                                     gagaATCGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGGTAACGTTGCTGCTGa                 >  1:391918/1‑140 (MQ=255)                                                                                                                          tCGTCAGGAAGTCGCTCAGACTCCTGTTGATCTGGCGGCCTTATTCCGTCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCgg            <  2:211897/140‑1 (MQ=255)                                                                                                                           cGTCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCggg           >  1:684743/1‑139 (MQ=255)                                                                                                                            gTCAGGAAGTCGCTCAGACTGCTGTCGATCTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCg          <  1:202756/140‑1 (MQ=255)                                                                                                                             tCAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGt         >  1:109429/1‑140 (MQ=255)                                                                                                                             ccAGGAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGtt        >  1:666438/2‑140 (MQ=255)                                                                                                                                 gAAGTCGCTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGCCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACt     >  1:630490/1‑140 (MQ=255)                                                                                                                                    gTCGCTCTGACTACTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGa  >  2:627454/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        AAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGA  >  NC_000913/4637531‑4637801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑