Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	605,132	A→G	V98A (GTT→GCT)	nfsB ←	NAD(P)H nitroreductase NfsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	605,132	0	A	G	100.0%	124.2 / NA	35	V98A (GTT→GCT)	nfsB	NAD(P)H nitroreductase NfsB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/15);  total (20/15)

CCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTT  >  NC_000913/604998‑605264                                                                                                                                       |                                                                                                                                     ccTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAAcagccagc                                                                                                                                 <  2:560692/140‑1 (MQ=255)   tGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCtt                                                                                                                               <  1:122864/140‑1 (MQ=255)   tGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCtt                                                                                                                               <  1:69410/140‑1 (MQ=255)              ccACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCAGCATCTTCCTGTTCGACAGCCTGCTTCAGCCAGACAt                                                                                                                    >  1:281819/1‑140 (MQ=255)                    tGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCa                                                                                                              >  1:53737/1‑140 (MQ=255)                      catcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATc                                                                                                            >  1:510110/1‑140 (MQ=255)                        tcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATcgc                                                                                                          <  2:492981/140‑1 (MQ=255)                           tcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGt                                                                                                       >  1:67786/1‑140 (MQ=255)                              tGCAGATCTTTACGGTGTATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGtttt                                                                                                    >  2:663203/1‑140 (MQ=255)                                     cTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAg                                                                                             >  2:524282/1‑140 (MQ=255)                                        tACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAac                                                                                          >  2:697278/1‑140 (MQ=255)                                           ggTGCATATCAGCGAAGAACTTTCGACCTTTCTCGATCGAGGCTTTCGGTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAAcacc                                                                                       <  2:278305/140‑1 (MQ=255)                                             tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAAcaccac                                                                                     >  1:530103/1‑140 (MQ=255)                                             tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAAcaccac                                                                                     >  1:551516/1‑140 (MQ=255)                                             tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAAcaccac                                                                                     >  1:636071/1‑140 (MQ=255)                                             tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAAcaccac                                                                                     >  1:651996/1‑140 (MQ=255)                                             tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAAcaccac                                                                                     >  1:399741/1‑140 (MQ=255)                                              gCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCACCGCGGTTTTTGCACAGAACACCacg                                                                                    >  1:278305/1‑140 (MQ=255)                                               cATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGttt                                                                                                     >  1:458697/1‑122 (MQ=255)                                               cATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGttt                                                                                                     <  2:458697/122‑1 (MQ=255)                                                       cGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAg                                                                           >  2:117784/1‑140 (MQ=255)                                                       cGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAg                                                                           <  1:477519/140‑1 (MQ=255)                                                       cGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCCTCCCTCGCGGTTTTTGCACAGAACACCACGACGTGCGAg                                                                           >  2:310628/1‑140 (MQ=255)                                                         aagaaCTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCATCAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGc                                                                         >  1:42548/1‑140 (MQ=255)                                                            aaCTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATc                                                                      <  1:117784/140‑1 (MQ=255)                                                              cTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCaa                                                                    <  1:594976/140‑1 (MQ=255)                                                              cTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCaa                                                                    <  2:666213/140‑1 (MQ=255)                                                                                cgcgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGACGCATCAAGCATTTTAGGCTCGTTGa                                                  >  1:727639/1‑140 (MQ=255)                                                                                  cgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAac                                                <  1:663203/140‑1 (MQ=255)                                                                                        tCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACg                                             <  2:365433/137‑1 (MQ=255)                                                                                        tCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACg                                             >  1:365433/1‑137 (MQ=255)                                                                                                 gCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCa                                 <  2:510110/140‑1 (MQ=255)                                                                                                 gCGTGGCAAAGCGGCCATCAGCATCTTCCTGGTCAACATCCATCTTCATCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCa                                 <  2:399741/140‑1 (MQ=255)                                                                                                                cATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAaca                  <  2:67786/140‑1 (MQ=255)                                                                                                                cATCGGCATCTTCCTGGTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGACAGCGGATTTGGCAaca                  <  2:530103/140‑1 (MQ=255)                                                                                                                      cATCTTCCTGGTCATCAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCt            <  2:42548/140‑1 (MQ=255)                                                                                                                                gTCAACAGCCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAAGTACTGCCGGCGGATTTAGCAATGCGCGCTTTACcttctt  >  2:355406/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     CCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTT  >  NC_000913/604998‑605264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑