Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	655,867	A→G	G34G (GGT→GGC)	dcuC ←	anaerobic C4‑dicarboxylate transporter DcuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	655,867	0	A	G	97.4%	135.8 / NA	39	G34G (GGT→GGC)	dcuC	anaerobic C4‑dicarboxylate transporter DcuC
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (14/24);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAA  >  NC_000913/655730‑655998                                                                                                                                          |                                                                                                                                    ccGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGgccgcc                                                                                                                                   >  2:515844/1‑140 (MQ=255)     ggTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCTATACTGGGACTGATAAGCAGCGATAACAGGCCGCCGACa                                                                                                                               >  2:193821/1‑140 (MQ=255)     ggTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACa                                                                                                                               <  1:528426/140‑1 (MQ=255)        cgccgccgcGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAAt                                                                                                                            <  1:579627/140‑1 (MQ=255)         gccgccgcGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATa                                                                                                                           >  2:381326/1‑140 (MQ=255)          ccgccgcGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATaa                                                                                                                          >  2:92591/1‑140 (MQ=255)                cgGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACAcc                                                                                                                    >  2:154826/1‑140 (MQ=255)                  gCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAg                                                                                                                  <  1:381326/140‑1 (MQ=255)                  gCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAg                                                                                                                  <  1:548705/140‑1 (MQ=255)                  gCTCATTAGTAATATTTAAACGCATTCAACGTTATTCGTAGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAg                                                                                                                  <  2:288157/140‑1 (MQ=255)                    tCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTg                                                                                                                <  2:458554/140‑1 (MQ=255)                        tAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATA‑‑ACACCAGTGGCGGaa                                                                                                          <  1:405139/140‑1 (MQ=255)                          gagacagtttAACGTCTTCAACTATCTCCATGGCGCTCTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGaa                                                                                                          <  1:207733/133‑1 (MQ=255)                          gTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGaa                                                                                                          <  1:92591/140‑1 (MQ=255)                          gTAATATGTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGaa                                                                                                          <  2:41391/140‑1 (MQ=255)                             atatTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATa                                                                                                        <  1:385842/139‑1 (MQ=255)                             atatTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATa                                                                                                        >  2:385842/1‑139 (MQ=255)                                ttttAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATAc                                                                                                       >  1:518458/1‑137 (MQ=255)                                ttttAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATAc                                                                                                       <  2:518458/137‑1 (MQ=255)                                ttttAACGTATTCAACGATATCCGTGGAGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATAAGCAATAAGAGGCCACCGACAAATAACACAGAAGTGGCGGAATACCCt                                                                                                    >  2:353409/1‑140 (MQ=255)                                            cAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGACCGCCGACGAATGACAGACCAGTGGAGGAAGACCCTTTAatctgtctc                                                                                        >  2:207733/1‑133 (MQ=255)                                                gATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGC‑‑AATACCCTTTAATGATGTAGCGAGCt                                                                                  <  2:133796/140‑1 (MQ=255)                                                  tatCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCt                                                                                  <  1:4835/140‑1 (MQ=255)                                                   atCCGTGGCGCTGTAACCTGTTAAACCCTCGCTGGACCGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTa                                                                                 <  1:193821/140‑1 (MQ=255)                                                    tCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTac                                                                                >  2:59640/1‑140 (MQ=255)                                                        tGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACAccc                                                                            <  2:390901/140‑1 (MQ=255)                                                                     tGTTGAAGCCTGGCTGGACGGTAACCCTTTTGGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACaa                                                               <  1:418767/140‑1 (MQ=255)                                                                     tGTTGAAGCCTGGCTGGACGGTAACCCTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACaa                                                               <  1:353409/140‑1 (MQ=255)                                                                     tGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCTCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACaa                                                               <  2:262442/140‑1 (MQ=255)                                                                               tggctggACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAg                                                     <  1:59640/140‑1 (MQ=255)                                                                                                  tgtgCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAACAACCACAACCCGAAAAAGGAGATCAATGAATGTCAGc                                  >  1:236039/1‑140 (MQ=255)                                                                                                    tgCCCCATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATATCCACAACCCCAGTACGGAGCTGAATGAATGTCAGCAt                                >  1:302292/1‑140 (MQ=255)                                                                                                        ccATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAAtt                            <  2:524878/140‑1 (MQ=255)                                                                                                        ccATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAAtt                            >  2:498042/1‑140 (MQ=255)                                                                                                         cATAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAAttt                           >  1:688644/1‑140 (MQ=255)                                                                                                          aTAATGGCACTGATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAGCCCCAATAAGGGGCGCAATGAGTGTCAGCATAAtttt                          >  1:206174/1‑140 (MQ=255)                                                                                                               ggCACTGATAATCAGCAATAACGGGCCGCCGACAACTAACACACCAGTAGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTg                     <  2:302292/140‑1 (MQ=255)                                                                                                                     gATAATCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCa               <  1:664770/140‑1 (MQ=255)                                                                                                                          tCAGCAATAACAGGCCGCCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGccc          >  1:626806/1‑140 (MQ=255)                                                                                                                            aGCAATAACAGGCCGCCCACAAATAACACACCAGTCGCGGAATACCCTATAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCa        <  2:236039/140‑1 (MQ=255)                                                                                                                                  aaCAGGCCGCCGACAAACAACAAACCAGTGGCGGACTACTCTTTAATGCTGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTaa  <  2:206174/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    CCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAA  >  NC_000913/655730‑655998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑