Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	93,241	A→G	T26A (ACA→GCA)	murE →	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	93,241	0	A	G	100.0%	117.9 / NA	33	T26A (ACA→GCA)	murE	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

GCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGC  >  NC_000913/93124‑93377                                                                                                                      |                                                                                                                                         gCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGGGTGGCTgc                                                                                                                    >  2:385927/1‑140 (MQ=255)    aTAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTgcggc                                                                                                                 >  2:708175/1‑140 (MQ=255)           tGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGAt                                                                                                          <  1:133933/140‑1 (MQ=255)           tGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGAt                                                                                                          <  1:578669/140‑1 (MQ=255)              aTTTGTGATTAATCATGGCGAGTGGTCAGCTGGCAGACCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGAtctc                                                                                                       <  1:335965/140‑1 (MQ=255)              aTTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGAAGGCACTCTACAGCCTTGTGGCTGCGGCGGGCGAtctc                                                                                                       >  1:354697/1‑140 (MQ=255)                ttGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCtt                                                                                                     <  2:123292/140‑1 (MQ=255)                   tgATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGt                                                                                                  <  2:652936/140‑1 (MQ=255)                        aaTCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTg                                                                                             >  2:668392/1‑140 (MQ=255)                             aGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTgtagta                                                                                        >  1:539072/1‑140 (MQ=255)                              ggCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGtagtag                                                                                       <  1:668392/140‑1 (MQ=255)                                    gggACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGTCTGGGGCCGGCGATCTCTTTGGAGCTGTAGTAGGtcatc                                                                                 >  2:273530/1‑140 (MQ=255)                                             ggCAGATCGTAATTTGCGCGACGTTCTTGCTACGTGGGTGCCAGACGCCCCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACg                                                                        <  2:225763/140‑1 (MQ=255)                                               cAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACggg                                                                      <  1:666817/140‑1 (MQ=255)                                                aGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGGCATCAGGcgggcgggg                                                                     >  2:479257/1‑139 (MQ=255)                                                 gATCGTAATTTGCGCTACCTTCTTGCTCCTTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                    <  1:273530/140‑1 (MQ=255)                                                 gATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                    <  1:97101/140‑1 (MQ=255)                                                 gATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                    <  2:629146/140‑1 (MQ=255)                                                  aTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGt                                                                   >  2:675989/1‑140 (MQ=255)                                                     gTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGa                                                                >  2:516539/1‑140 (MQ=255)                                                                      cttGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGAt                                               <  1:282326/140‑1 (MQ=255)                                                                             cGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCaa                                        >  2:486829/1‑140 (MQ=255)                                                                               tGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGGGGACGGGGGTCGATATATCCCTCAGGCGATAGCGCAAgg                                      >  2:302072/1‑140 (MQ=255)                                                                               tGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAgg                                      >  1:21618/1‑140 (MQ=255)                                                                                         aCGCACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGGCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGcc                            >  2:89243/1‑140 (MQ=255)                                                                                            cACCTTCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGAGGGGCGTCGATATGTACGGCAGGCGATAGCGTGAGGTGTGGCTGCCatt                         >  2:335110/1‑140 (MQ=255)                                                                                                ttCGCGAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGGCGATATATCCCGCAGGCGATAGCGCAAAGGGTGGCCGCCcttattt                     >  2:158593/1‑139 (MQ=255)                                                                                                  cgcgAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCa                   <  2:499346/140‑1 (MQ=255)                                                                                                  cgcgAGCACTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCa                   <  1:506193/140‑1 (MQ=255)                                                                                                         aCTGCGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGa            >  1:470154/1‑140 (MQ=255)                                                                                                             cGAGAGATGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGGCATCAGGCGGACGGGCGTCGATATATCCGGCAGGCGATAGCTCAACTTGTGGCTGCCATTATTGCAGAAGCGAAAGa        >  2:341791/1‑140 (MQ=255)                                                                                                                 agaTGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAg    <  1:516539/140‑1 (MQ=255)                                                                                                                   aTGGCACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGc  <  2:539072/140‑1 (MQ=255)                                                                                                                      |                                                                                                                                         GCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGC  >  NC_000913/93124‑93377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑