Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 817,144 A→G D34G (GAT→GGT)  moaA → GTP 3',8'‑cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913817,1440AG98.1% 182.4 / NA 53D34G (GAT→GGT) moaAGTP 3',8'‑cyclase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (25/27);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTCCCGTATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGAC  >  NC_000913/817019‑817280
                                                                                                                             |                                                                                                                                        
gCCTCCCGTATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATCTGTGTAACTTTCGTTGCACCTACTGGCTGCCGGGTGGCTACAAACCGa                                                                                                                            >  2:268068/1‑140 (MQ=255)
   tCCCGTATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAgcg                                                                                                                         <  1:446123/140‑1 (MQ=255)
    cccGTATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAgcgg                                                                                                                        >  1:12718/1‑140 (MQ=255)
     ccGTATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAgcggc                                                                                                                       <  2:662581/140‑1 (MQ=255)
     ccGTATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAgcggc                                                                                                                       <  2:456547/140‑1 (MQ=255)
       gTATCTGGAAAGGTGCACATGGATTGACAGCTGACTGCTGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGt                                                                                                                     <  1:343611/140‑1 (MQ=255)
        tATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCt                                                                                                                                     <  2:59435/123‑1 (MQ=255)
        tATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCt                                                                                                                                     >  1:59435/1‑123 (MQ=255)
        gaTCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTc                                                                                                                    <  2:695604/139‑1 (MQ=255)
          tCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCa                                                                                                                   <  2:538796/139‑1 (MQ=255)
          tCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCa                                                                                                                   >  1:538796/1‑139 (MQ=255)
            tGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCa                                                                                                                >  1:124699/1‑140 (MQ=255)
               aaaGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGTCTGCCGGATGGGTACAAACCGTGCGGCGTCACCAATa                                                                                                             >  1:239416/1‑140 (MQ=255)
                   gtgtACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAgg                                                                                                         <  1:536226/140‑1 (MQ=255)
                   gtgtACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAgg                                                                                                         >  2:442503/1‑140 (MQ=255)
                    tgtACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACATACTGCCTGCCGGGTGGCTACAAACCGAGCAGCGTCACCAATAAAGGc                                                                                                        >  2:299489/1‑140 (MQ=255)
                      tACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCtt                                                                                                      >  1:581597/1‑140 (MQ=255)
                           ggCTTCACAACTGACTGATGCATTTCCGCATAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTa                                                                                                 <  1:234895/140‑1 (MQ=255)
                            gCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTAc                                                                                                >  1:412657/1‑140 (MQ=255)
                             cTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACCAGCCGAGCGGCGCCACCAGTAAAGGCGTTCTTAcc                                                                                               >  2:357784/1‑140 (MQ=255)
                             cTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTAcc                                                                                               >  2:54457/1‑140 (MQ=255)
                                cacaACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCTGCGGCACCAATAAGGGCTTTCTTACCGTc                                                                                            >  1:255484/1‑140 (MQ=255)
                                cacaACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTAAAAAGCGAGCGGCGTCAACAATGAAGGCTTTCTTACCGTc                                                                                            >  2:212413/1‑140 (MQ=255)
                                  caACTGACTGATGCATTTGCGCTTAAGTTTTTCTACTTGCGCCTGTCGGTTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGa                                                                                          <  2:255484/140‑1 (MQ=255)
                                          tgaTGCATTTGCGCGAAAGTTTCACTACTTGCGCCTGCCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTc                                                                                  <  1:212413/140‑1 (MQ=255)
                                                       cgTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTAcgcg                                                                     >  1:110488/1‑140 (MQ=255)
                                                       cgTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGGCACCAATAGAGGATTTATTACCGTCGATGAAATTCGCCGGGTTAcgcg                                                                     >  2:259139/1‑140 (MQ=255)
                                                          aaGTTTTACTACTTGCGCCTGTGGATTACCGCTGTCTGTCACTTTCGTCGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACgcgcgc                                                                  <  2:259439/140‑1 (MQ=255)
                                                          aaGTTTTACTACTTGAGCCTGTCGACTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACgcgcgc                                                                  <  1:305225/140‑1 (MQ=255)
                                                             ttttACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCtt                                                               <  2:412657/140‑1 (MQ=255)
                                                               ttACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCg                                                             <  2:12718/140‑1 (MQ=255)
                                                                       tGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTAGCCAGGATACGCGAGCCTTCGCCAGACTg                                                     >  1:304652/1‑140 (MQ=255)
                                                                         cgcCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTggg                                                   <  2:581597/140‑1 (MQ=255)
                                                                           ccTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCa                                                 <  2:462866/140‑1 (MQ=255)
                                                                             tGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCAcc                                               >  2:180786/1‑140 (MQ=255)
                                                                               tCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTTGCCGGGTTACGCGCTCCTTCGTCAGACTGGGCACCGa                                             >  1:329611/1‑140 (MQ=255)
                                                                                 gATTACCGATGTGTGTATCTTTAGTTGCACCTACTGCCTGCCGGGTGGTTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaa                                           <  2:304652/140‑1 (MQ=255)
                                                                                      ccGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTg                                      <  1:180786/140‑1 (MQ=255)
                                                                                         aTGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTgcgc                                   >  1:396463/1‑140 (MQ=255)
                                                                                         aTGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTgcgc                                   <  1:442503/140‑1 (MQ=255)
                                                                                           gtgtgtAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCt                                 <  1:54457/140‑1 (MQ=255)
                                                                                              tgtAACTTTCGTTGCCCCTACTACCCGCCAGGTGGCTTCAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGAc                              <  1:352415/140‑1 (MQ=255)
                                                                                                tAACTTTCGTTGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACag                            >  2:541021/1‑140 (MQ=255)
                                                                                                tAACTTTCCTTGTACCTCCTGCCTGCCGGGTGGATACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACag                            <  2:685235/140‑1 (MQ=255)
                                                                                                     ttCGTTGCACCTACTCCCTGCCGGGAGGCAACAAACCTAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAggagga                       <  1:259139/140‑1 (MQ=255)
                                                                                                         ttGCACCTACTGCCTGCCGGGTGGCTACAAACCGAGTGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGc                   <  1:546284/140‑1 (MQ=255)
                                                                                                             aCCTACTGCCTGCCGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGGTGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGtt               >  1:684936/1‑139 (MQ=255)
                                                                                                                    gcctgccGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTAcgc        <  2:54217/140‑1 (MQ=255)
                                                                                                                       tgccGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTAcgccgc     >  2:461004/1‑140 (MQ=255)
                                                                                                                         ccGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGa   >  1:71803/1‑140 (MQ=255)
                                                                                                                         ccGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGa   >  2:583402/1‑140 (MQ=255)
                                                                                                                         ccGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGa   >  1:636935/1‑140 (MQ=255)
                                                                                                                          cGGGTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGAc  <  2:110488/140‑1 (MQ=255)
                                                                                                                             |                                                                                                                                        
GCCTCCCGTATCTGGAAAGGTGTACATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGAC  >  NC_000913/817019‑817280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑