Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	854,750	A→G	I368M (ATA→ATG)	ybiR →	putative transporter YbiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	854,750	0	A	G	100.0%	120.3 / NA	35	I368M (ATA→ATG)	ybiR	putative transporter YbiR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/16);  total (19/16)

CGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGT  >  NC_000913/854624‑854885                                                                                                                               |                                                                                                                                        cGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACt                                                                                                                            >  1:44681/1‑140 (MQ=255)         tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTg                                                                                                                    >  1:61725/1‑140 (MQ=255)                     ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGTTTCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATaaaaaa                                                                                                        <  2:636189/140‑1 (MQ=255)                       gcgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                                                      <  2:61725/140‑1 (MQ=255)                               tATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTAt                                                                                              <  1:688359/140‑1 (MQ=255)                                  ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCGAACTAGGGTCTAGCCAATAAAAAAGGCGGATTATGAg                                                                                           >  1:260849/1‑140 (MQ=255)                                       aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGc                                                                                      >  2:420085/1‑140 (MQ=255)                                       aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGc                                                                                      >  2:406675/1‑140 (MQ=255)                                       aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGc                                                                                      >  1:404729/1‑140 (MQ=255)                                        cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGcc                                                                                     >  2:114295/1‑140 (MQ=255)                                             gccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTg                                                                                >  2:154654/1‑140 (MQ=255)                                                         ggCGTTTCCATCTCTATTCAATACCGATGCTGCTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                    <  2:497912/140‑1 (MQ=255)                                                                         ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACg                                                    <  2:404729/140‑1 (MQ=255)                                                                              tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGGCTTATGCTTTAAGTTTAGAAGGTTAATTCAGACGAACCg                                               >  2:239668/1‑140 (MQ=255)                                                                              tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                               >  2:91278/1‑140 (MQ=255)                                                                              tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                               >  1:390470/1‑140 (MQ=255)                                                                               aCCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCgg                                              <  2:390470/140‑1 (MQ=255)                                                                                 cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCa                                            >  1:104363/1‑140 (MQ=255)                                                                                   aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCACATCAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                          >  1:407987/1‑140 (MQ=255)                                                                                   aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                          >  2:697350/1‑140 (MQ=255)                                                                                   aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                          >  2:143326/1‑140 (MQ=255)                                                                                   aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                          >  1:523963/1‑140 (MQ=255)                                                                                   aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                          >  2:601013/1‑140 (MQ=255)                                                                                   aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                          >  2:526591/1‑140 (MQ=255)                                                                                   aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGCCCGCCTTTAGCTTTAAGTTTTGAAGGTTAACTCAGACGAACCGGCATc                                          >  2:311244/1‑140 (MQ=255)                                                                                     gCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGTTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATccc                                        <  1:406675/140‑1 (MQ=255)                                                                                         ttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                    <  1:91278/140‑1 (MQ=255)                                                                                                   cgTTGGTGGGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                          <  1:114295/140‑1 (MQ=255)                                                                                                        gtggGATATGTTTTGTTAGTTATGCTCCCGGCCAACTGGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                     <  1:320463/140‑1 (MQ=255)                                                                                                        gtggGATATGTTTTGTTAGTTATGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                     <  1:601013/140‑1 (MQ=255)                                                                                                        gtggGATATGTTTTGTTAGTTATGCACCCGGCCAACTCGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                     <  1:266175/140‑1 (MQ=255)                                                                                                                  ttttGTTACTTATGCTTCCGGCGAACTAGGGTCGGACAACTAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaa           <  2:334652/140‑1 (MQ=255)                                                                                                                      gttagttaTGCTCCCGGCCAACGAGGGTCTGCCAACTAAGAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac       <  2:260849/140‑1 (MQ=255)                                                                                                                       ttagttaTGCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAAcc      <  2:104363/140‑1 (MQ=255)                                                                                                                           ttaTGCTCCCGGCCACCTAGGGCCTGGCACATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt  <  1:239668/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                        CGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGT  >  NC_000913/854624‑854885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑