Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	859,698	T→C	S111G (AGT→GGT)	ybiV ←	sugar phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	859,698	0	T	C	100.0%	144.5 / NA	42	S111G (AGT→GGT)	ybiV	sugar phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/19);  total (23/19)

TCCGGCAGGTTGAGCGAAAACTTGAACAGTACGTCGTCAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACTTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCATGTTCGTAAACCAGTGCGCCGTTTTCCG  >  NC_000913/859565‑859832                                                                                                                                      |                                                                                                                                       tCCGGCAGGTTGAGCGAAAACTTGAACTGTACGTCGGCAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAACTGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAg                                                                                                                                  >  2:29658/1‑140 (MQ=255)     gCAGGTTGAGCGAAAACTTGAACAGTACGTCGTCAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCg                                                                                                                              >  1:632194/1‑140 (MQ=255)         gTTGAGCGAAAACTTGAACAGTACGTCGTCAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAgg                                                                                                                          <  1:487686/140‑1 (MQ=255)                      ttGAACAGTACGTCGTCAATCTCCTGATAATCTTTTACAGGATTCAGGCGATGGTAGTGTTTTGCCATCAGAGCGACAAATGCTACGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCTGGCGACAAAATGGAg                                                                                                             <  1:29658/140‑1 (MQ=255)                           cAGTACGTCGTCAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCt                                                                                                        <  2:562501/140‑1 (MQ=255)                                   cgtcAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCtttt                                                                                                >  1:607426/1‑140 (MQ=255)                                      cAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGc                                                                                             >  2:570909/1‑140 (MQ=255)                                      cAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGc                                                                                             >  2:137380/1‑140 (MQ=255)                                      cAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACAGCAGACGGCAGAACTGAGATTGTTATCTTTTAGc                                                                                             >  2:678518/1‑140 (MQ=255)                                        aTCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCaa                                                                                           >  1:367160/1‑140 (MQ=255)                                         tctcCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAAc                                                                                          <  2:80212/140‑1 (MQ=255)                                         tctcCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAAc                                                                                          <  2:632194/140‑1 (MQ=255)                                                    tCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAAc                                                                               <  2:607426/140‑1 (MQ=255)                                                                ttCAGGCTATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTc                                                                   >  1:651082/1‑140 (MQ=255)                                                                  cAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCAt                                                                 >  2:319897/1‑140 (MQ=255)                                                                  cAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAAGTGAGTTGCGTATTTTTATGCAACTCGCCAAATACAATCCACGATTCAt                                                                 >  2:354799/1‑140 (MQ=255)                                                                        aTGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCggg                                                           >  1:419489/1‑140 (MQ=255)                                                                        aTGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCggg                                                           >  1:1939/1‑140 (MQ=255)                                                                          ggTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGTTTATCTTTAAGCTACTCGCCAATAACAATCCGCGATTCATGTCGGGTc                                                         >  2:269465/1‑140 (MQ=255)                                                                          ggTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGTCAAAAAAAATCCGTGATTCATGTCGGGTc                                                         >  1:231192/1‑140 (MQ=255)                                                                              gtgtTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGtt                                                     <  2:68522/140‑1 (MQ=255)                                                                                 ttttGCCATCAGTGCGACAAATTCTTCGGGGCCATTTTCGCTGACATAGGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGc                                                  <  2:231192/140‑1 (MQ=255)                                                                                   ttGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGtt                                                     <  2:91676/135‑1 (MQ=255)                                                                                   ttGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGtt                                                     <  2:506722/135‑1 (MQ=255)                                                                                   ttGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGtt                                                     >  1:91676/1‑135 (MQ=255)                                                                                   ttGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGtt                                                     >  1:506722/1‑135 (MQ=255)                                                                                   ttGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCg                                                >  2:48974/1‑140 (MQ=255)                                                                                    tGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGt                                               >  1:31951/1‑140 (MQ=255)                                                                                          cAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGaaca                                         >  1:713910/1‑140 (MQ=255)                                                                                             tGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACt                                      >  2:556159/1‑140 (MQ=255)                                                                                                  cAAATGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTg                                 >  1:1666/1‑140 (MQ=255)                                                                                                     aTGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCa                              >  2:10095/1‑140 (MQ=255)                                                                                                      tGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCAt                             <  2:367160/140‑1 (MQ=255)                                                                                                      tGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCAt                             <  2:567156/140‑1 (MQ=255)                                                                                                      tGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCAt                             <  1:678518/140‑1 (MQ=255)                                                                                                      tGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCAt                             <  1:570909/140‑1 (MQ=255)                                                                                                      tGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCAt                             <  1:535942/140‑1 (MQ=255)                                                                                                      tGCTTCGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCAt                             <  1:137380/140‑1 (MQ=255)                                                                                                           cGGGGGCATTTTCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCATGTTCg                        >  1:714202/1‑140 (MQ=255)                                                                                                                   ttttCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCATGTTCGTAAACCAg                >  2:508934/1‑140 (MQ=255)                                                                                                                      tCGCTGACATATGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCATGTTCGTGAACCAGTgc             <  1:556159/140‑1 (MQ=255)                                                                                                                               tatGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGATCAGTTCGCCGTGGAACAACTGCTTGCCATGTTCGTAAACCAGTGCGCCGTTTTc    <  1:10095/140‑1 (MQ=255)                                                                                                                                 tGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCATGTTCGTAAACCAGTGCGCCGTTTTCcg  <  2:31951/140‑1 (MQ=255)                                                                                                                                 tGCACCTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCATGTTCGTAAACCAGTGCGCCGTTTTCcg  <  1:410118/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TCCGGCAGGTTGAGCGAAAACTTGAACAGTACGTCGTCAATCTCCTGATAATCTTTTACAGGTTTCAGGCGATGGTAGTGTTTTGCCATCAGTGCGACAAATGCTTCGGGGGCATTTTCGCTGACATATGCACTTTGCAGACCGCAGGCGACAAAATTGAGTTGCTTATCTTTTAGCAACTCGCCAATAACAATCCGCGATTCATGTCGGGTCAGTTCGCCGTGGAACAACTGCTTGCCATGTTCGTAAACCAGTGCGCCGTTTTCCG  >  NC_000913/859565‑859832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑