Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	886,543	A→G	V266A (GTT→GCT)	ybjJ ←	inner membrane protein YbjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	886,543	0	A	G	100.0%	94.8 / NA	27	V266A (GTT→GCT)	ybjJ	inner membrane protein YbjJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

ACACCCCAGCGACCCAGGCGCTATCGACAAAAATAATCAGCCCAATACCCAACGCCCCCATTAGTGCACTGGCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAACGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACCAGTCGTTGGCAGAACCTTCGGCAAAGGCCATCGCCAGCACCACAA  >  NC_000913/886412‑886675                                                                                                                                    |                                                                                                                                     acacCCCAGCGACCCAGGCGCTATCGACAAAAATAATCAGCCCAATACCCAACGCCCCCATTAGTGCACTGGCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATc                                                                                                                              >  1:738378/1‑140 (MQ=255) acacCCCAGCGACCCAGGCGCTATCGACAAAAATAATCAGCCCAATACCCAACGCCCCCATTAGTGCACTGGCCCGAACCACGGCAACGCGACTGTAACGGTAGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATc                                                                                                                              >  2:505782/1‑140 (MQ=255)              ccAGGCGCTATCGAAAAAAATAAACGGCCAAACCCCCGCCCCCCCCATTAGTCCTCTGGCCCGAACCGCGGCACCGCGTGTGAAACGGGCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAAc                                                                                                                 <  2:682331/140‑1 (MQ=255)                     cTATCGACAAAAATAATCAGCCCAATACCCAACGCCCCCATTAGTGCACTGGCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATa                                                                                                          >  1:467594/1‑140 (MQ=255)                           aaaaaaaTACCCAGCCCAACCCCCACCCCCCCCATTAGTGCACGGCCCCGACCCACGGCAACGCGACTGTACCGGTCAATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAg                                                                                                    <  2:440680/138‑1 (MQ=255)                            aaaaaaTAATCACCCCAGCCCCCACCCCCCCCATTAGTCTACGGCCCCCAACCCCGGCAACGCGACTGAAACGGTCGAGAAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGc                                                                                                   <  2:279769/139‑1 (MQ=255)                               aaaaaaCCACCCCAACCCCCACCCCCCCAATAACTGCACGGGCCCAACCCACGCCAACGCGACTGAACCGGCCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAg                                                                                                <  2:738378/140‑1 (MQ=255)                                           cAATACCCAACGCCCCCATTAGTGCACTGGCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAAGGAAAAATCGGCATAAATCACGGACCCGGAAGTAggg                                                                                    >  1:296350/1‑140 (MQ=255)                                                      gCCCCCATTAGTGCACTGGCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTg                                                                         >  1:104156/1‑140 (MQ=255)                                                             ttAGTGCCCGGGCCCGACCCCGGGCAGGGCGACTGAAACGGGCGATAAACCAGCCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCa                                                                  <  2:587757/140‑1 (MQ=255)                                                             ttAGCGCCCGGGCCCGACCCACGGCAACGCGACTGAAACGGTCGATAAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCa                                                                  <  2:586928/140‑1 (MQ=255)                                                                gTGCACGGGCCCGACCCGCGGCAACGGGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAAcc                                                               <  2:104156/140‑1 (MQ=255)                                                                     cTGGCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATtaa                                                          >  1:586928/1‑140 (MQ=255)                                                                     cTGCCCCGACCCACGGCAACGCGATTGAAACGGTCGATAAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATtaa                                                          <  2:560785/140‑1 (MQ=255)                                                                       ggCCCGACCCAGGGAACCGCGATTGAACCGGTGGAGAAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATtaata                                                        <  2:437495/140‑1 (MQ=255)                                                                        gCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATtaataa                                                       >  1:92379/1‑140 (MQ=255)                                                                        gCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATtaataa                                                       >  1:560785/1‑140 (MQ=255)                                                                        gCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATtaataa                                                       >  1:437495/1‑140 (MQ=255)                                                                        gCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATtaa                                                          >  1:662693/1‑137 (MQ=255)                                                                        gCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATtaa                                                          <  2:662693/137‑1 (MQ=255)                                                                                  cGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGt                                                   <  2:687856/134‑1 (MQ=255)                                                                                  cGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGt                                                   >  1:687856/1‑134 (MQ=255)                                                                                  cGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACCAg                                             >  2:655506/1‑140 (MQ=255)                                                                                                     gTCGATGAACCAACCGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACCAGTCGTTGGCAGAACCTTCgg                          >  1:401557/1‑140 (MQ=255)                                                                                                             accaaccGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACCAATCGTTGGCAGAACCTTCGGCAAAGAcc                  <  2:92379/140‑1 (MQ=255)                                                                                                                 accGCCGGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACCAGACGTTGGCCGAACCATCGGCAAAGGCCATCg              >  2:341134/1‑140 (MQ=255)                                                                                                                    gccgGTGAAGCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACCAGTCGTTGGCAGAACCTTCGGCAAAGGCCATCGCCa           >  1:90590/1‑140 (MQ=255)                                                                                                                             gCGTCCAGCGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACCAGTCGTTGGCAGAACCTTCGGCAAAGGCCATCGCCAGCACCacaa  >  2:119027/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     ACACCCCAGCGACCCAGGCGCTATCGACAAAAATAATCAGCCCAATACCCAACGCCCCCATTAGTGCACTGGCCCGAACCACGGCAACGCGACTGTAACGGTCGATGAACCAACCGCCGGTGAAGCGTCCAACGGTCATCCCCAGGGTAAAACCGGCATAAATCAGCGAGCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACCAGTCGTTGGCAGAACCTTCGGCAAAGGCCATCGCCAGCACCACAA  >  NC_000913/886412‑886675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑