Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	888,832	A→G	F330L (TTC→CTC)	ybjL ←	putative transport protein YbjL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	888,832	0	A	G	100.0%	135.1 / NA	39	F330L (TTC→CTC)	ybjL	putative transport protein YbjL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/25);  total (14/25)

GAGATAAAGCCGATGCGATCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAAGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCATGTCGAGAAGGTCACGATCGAAAACTTCTTTACCGTTACG  >  NC_000913/888696‑888961                                                                                                                                         |                                                                                                                                  gagaTAAAGCCGATGCGATCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCa                                                                                                                                >  1:35644/1‑140 (MQ=255)  agaTAAAGCCGATGCGATCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCaa                                                                                                                               <  2:483878/140‑1 (MQ=255)  agaTAAAGCCGATGCCATCGGCTATAGTTCTTACGCGCCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCaa                                                                                                                               <  1:190143/140‑1 (MQ=255)        aGCCGATGCGATCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGa                                                                                                                         <  1:280474/140‑1 (MQ=255)            gATGCGATCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCCGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCgg                                                                                                                     <  2:421357/140‑1 (MQ=255)             aTGCGATCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGt                                                                                                                    >  1:32449/1‑140 (MQ=255)             aTGCGATCGGCGATGG‑TTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTc                                                                                                                   <  2:39890/140‑1 (MQ=255)              tGCGATCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTc                                                                                                                   <  2:75899/140‑1 (MQ=255)                  aTCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACt                                                                                                               >  2:612897/1‑140 (MQ=255)                              ttttACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCga                                                                                                   <  1:10836/140‑1 (MQ=255)                              ttttACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACATTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCga                                                                                                   <  1:644484/140‑1 (MQ=255)                                ttACGCGGCGGGCATCGGCGCTCACTTGTAAAACGCCACCTTTGTTAACCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCgaga                                                                                                 <  2:217258/140‑1 (MQ=255)                                ttACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCgaga                                                                                                 <  2:29460/140‑1 (MQ=255)                                ttACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCgaga                                                                                                 <  1:435014/140‑1 (MQ=255)                                    gcggcgGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGtt                                                                                             <  2:674750/140‑1 (MQ=255)                                     cggcggGCATCGCCGCTGACTTGTAAAACGTCACC‑TTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGATTAAGGAGGCAAACGTGATCGGTCATCTTCGGTTGTGCGAGACGTTTa                                                                                           >  2:292446/1‑140 (MQ=255)                                      ggcggGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCgaga                                                                                                 <  2:387524/134‑1 (MQ=255)                                      ggcggGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCgaga                                                                                                 >  1:387524/1‑134 (MQ=255)                                      ggcggGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGAACGGACAACTTCAGATGTGCGAGACGTTTa                                                                                           >  1:217258/1‑140 (MQ=255)                                                              aaaCGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACg                                                                   <  2:35644/140‑1 (MQ=255)                                                                aCGTCACCTTTGTTAGGCACGAAGTAGTCATCTATCGCCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGAc                                                                 <  1:267449/140‑1 (MQ=255)                                                                aCGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCTTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGAc                                                                 <  1:61774/140‑1 (MQ=255)                                                                aCGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGAc                                                                 <  2:29856/140‑1 (MQ=255)                                                                 cGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGAcc                                                                >  1:68610/1‑140 (MQ=255)                                                                   tCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCAc                                                              >  2:142385/1‑140 (MQ=255)                                                                      ccTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCATTTGTGCGAAACGGTTAGCTACAGCGTTATGATTTTTAACGACCActtc                                                           >  2:267449/1‑140 (MQ=255)                                                                            ttAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGt                                                     <  1:119820/140‑1 (MQ=255)                                                                              aaGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGa                                                   <  1:612897/140‑1 (MQ=255)                                                                                gCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACg                                                 >  2:681645/1‑140 (MQ=255)                                                                                   cgacgTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATa                                              >  2:531703/1‑140 (MQ=255)                                                                                    gacgTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAACGTCATGGTTTTTAACCACCACTTCTTCAGTGACGATAc                                             >  2:252183/1‑140 (MQ=255)                                                                                    gacgTTGTCATCTATCGCCATCTCAATCTGGCTACCAATCACGCGGTTCAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATAc                                             <  2:196929/140‑1 (MQ=255)                                                                                     acgTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGGTATGGTTATTAGCGGCCACTTTTTCAGTGACGATACg                                            >  1:196929/1‑140 (MQ=255)                                                                                             aTCTATCGGCATCTCTATCTGGCTATGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCATGTCga                                    <  1:311858/140‑1 (MQ=255)                                                                                              tCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCATGTCgag                                   <  2:574079/140‑1 (MQ=255)                                                                                              tCTATCGGCATCTCAATCTGGCAACGAATGACGCGGTCAAGGAGGCAACCGTGATCGGTCAACTTCGGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCAAGTCgag                                   <  1:292446/140‑1 (MQ=255)                                                                                                                   cTACGAATGACGCGGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCATGTCGAGAAGGTCACGATCGAAAActtc              >  2:577196/1‑140 (MQ=255)                                                                                                                       gAATGACGCGGTTAATGAGGCAACCTTGAACGGTCAAGTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCATGTCGAGAAGGTCACGATCGAAAACTTCTTTa          <  1:252183/140‑1 (MQ=255)                                                                                                                               cgGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCATGTCGAGAAGGTCACGATCGAAAACTTCTTTACCGTTACg  <  1:531703/140‑1 (MQ=255)                                                                                                                               cgGTTAAGGAGGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCATGTCGAGAAGGTCACGATCGAAAACTTCTTTACCGTTACg  <  1:411707/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GAGATAAAGCCGATGCGATCGGCGATGGTTTTTACGCGGCGGGCATCGCCGCTGACTTGTAAAACGTCACCTTTGTTAAGCACGACGTTGTCATCTATCGGCATCTCAATCTGGCTACGAATGACGCGGTTAAGGAAGCAACCGTGATCGGTCAACTTCAGTTGTGCGAGACGTTTACCTACAGCGTTATGGTTTTTAACGACCACTTCTTCAGTGACGATACGCATGTCGAGAAGGTCACGATCGAAAACTTCTTTACCGTTACG  >  NC_000913/888696‑888961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑