Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	103,198	T→C	V15A (GTT→GCT)	ftsQ →	cell division protein FtsQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	103,198	0	T	C	100.0%	102.1 / NA	30	V15A (GTT→GCT)	ftsQ	cell division protein FtsQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/14);  total (16/14)

CCACAGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGCTGGATGGAAGATGCGCAACGCCTGCCGCTC  >  NC_000913/103069‑103325                                                                                                                                  |                                                                                                                                ccACAGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTcgc                                                                                                                       <  2:63608/140‑1 (MQ=255) ccACAGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTcgc                                                                                                                       <  2:138008/140‑1 (MQ=255) ccACAGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTcgc                                                                                                                       >  1:35143/1‑140 (MQ=255) ccACAGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTcgc                                                                                                                       >  1:616531/1‑140 (MQ=255)   acaGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTcgccg                                                                                                                     <  2:35143/140‑1 (MQ=255)     aGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCa                                                                                                                   >  1:550970/1‑140 (MQ=255)           gTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATg                                                                                                             >  2:551205/1‑140 (MQ=255)                              aggcaggTATGAGCTCCTCGCATTTCGTACTACGAATCCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGAt                                                                                          <  1:201407/140‑1 (MQ=255)                                  aggTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCtt                                                                                      <  2:550970/140‑1 (MQ=255)                                        gAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCctg                                                                                >  1:456632/1‑140 (MQ=255)                                            ttCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGa                                                                            <  2:616531/140‑1 (MQ=255)                                              ctcGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGAcc                                                                          >  2:397329/1‑140 (MQ=255)                                              ctcGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGAcc                                                                          >  2:108591/1‑140 (MQ=255)                                                     ttGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTaa                                                                   <  1:551205/140‑1 (MQ=255)                                                      tGGTAGTACGAATTCTGGAACTCGCGGACTAGTATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAAc                                                                  <  2:345274/140‑1 (MQ=255)                                                        gtagtaCGAATTCTGGAACTGGCGGACTAATATGTCGGAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGa                                                                <  1:284963/140‑1 (MQ=255)                                                        gtagtaCGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGa                                                                >  2:64486/1‑140 (MQ=255)                                                             aCGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAgtg                                                           >  1:615358/1‑140 (MQ=255)                                                               gAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACCGGGtt                                                         >  2:651673/1‑140 (MQ=255)                                                                        aaCTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCgg                                                <  2:615358/140‑1 (MQ=255)                                                                                       atGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTggg                                 >  2:613727/1‑140 (MQ=255)                                                                                       atGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTggg                                 <  1:397329/140‑1 (MQ=255)                                                                                        tGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGc                                <  1:64486/140‑1 (MQ=255)                                                                                        tGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGc                                <  1:42930/140‑1 (MQ=255)                                                                                                   gacaggAACACGCGATACAGCGAAGTAGACGCTTCTTTTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGCTGGATGGAAGa                     <  1:189882/135‑1 (MQ=255)                                                                                                    ctctGAACACGCGAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGCTGGATGGAAGAt                    >  2:394837/1‑140 (MQ=255)                                                                                                              gcgAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGTCAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGCTGGATGGAAGATGCGCAACGcc          >  1:181763/1‑140 (MQ=255)                                                                                                              gcgAAACAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACCGTGTTGGTGAGCGGCTGGGTCGTGTTGGGCTGGATGGAAGATACGCAACGcc          >  2:383279/1‑140 (MQ=255)                                                                                                                 aaaCAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGCTGGATGGAAGATGCGCAACGCCTGc       <  2:181763/140‑1 (MQ=255)                                                                                                                   aCAGCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTTAGCGGCTGGGTCGTGTCGGGCTGGAAGGAAGATGCGCAACGccctgtc     >  2:189882/1‑135 (MQ=255)                                                                                                                      gCGAAGAAGAGGCTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGCTGGATGGAAGATGCGCAACGCCTGCCGctc  >  2:512616/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                CCACAGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTTCTCGCCGCAATAATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTGAGCGGCTGGGTCGTGTTGGGCTGGATGGAAGATGCGCAACGCCTGCCGCTC  >  NC_000913/103069‑103325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑