Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	913,631	C→T	V62I (GTT→ATT)	hcp ←	protein S‑nitrosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	913,631	0	C	T	100.0%	87.1 / NA	26	V62I (GTT→ATT)	hcp	protein S‑nitrosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/10);  total (16/10)

CCAGGCATTGCGCTTTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAG  >  NC_000913/913505‑913761                                                                                                                               |                                                                                                                                   ccAGGCATTGCGCTTTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTtgatg                                                                                                                       >  1:403974/1‑140 (MQ=255)             cTTTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCa                                                                                                           <  2:373780/140‑1 (MQ=255)                         cgcgCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTAcc                                                                                               >  1:535634/1‑140 (MQ=255)                                          tCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGccc                                                                              >  2:385063/1‑140 (MQ=255)                                           cACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCt                                                                             >  2:18838/1‑140 (MQ=255)                                               aGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCa                                                                         <  1:595679/140‑1 (MQ=255)                                                   tAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAgcgc                                                                     >  1:567192/1‑140 (MQ=255)                                                     gCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGcgccg                                                                   >  1:532656/1‑140 (MQ=255)                                                      ccGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGcgccgc                                                                  <  1:18838/140‑1 (MQ=255)                                                       cGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCcgcg                                                                 <  2:535634/140‑1 (MQ=255)                                                        gACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAgcgc                                                                     >  2:415568/1‑135 (MQ=255)                                                        gACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGATGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAgcgc                                                                     <  1:415568/135‑1 (MQ=255)                                                         aCAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGAt                                                               >  2:613258/1‑140 (MQ=255)                                                             tACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGt                                                           <  1:53041/140‑1 (MQ=255)                                                                     gaATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTg                                                   <  2:371287/140‑1 (MQ=255)                                                                                aaCGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAg                                        >  2:493718/1‑140 (MQ=255)                                                                                aaCGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGCCAGAAAGCCCTTTCTGCTCCGCGATAAGTAGCTCCTGAAAGTCAGAAg                                        >  2:353919/1‑140 (MQ=255)                                                                                      ggTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGTAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccg                                  >  2:473922/1‑140 (MQ=255)                                                                                      ggTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccg                                  >  2:714238/1‑140 (MQ=255)                                                                                      ggTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccg                                  >  1:384720/1‑140 (MQ=255)                                                                                                 agaAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccgc                                 >  2:468061/1‑130 (MQ=255)                                                                                                 agaAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccgc                                 <  1:468061/130‑1 (MQ=255)                                                                                                 agaAAAAGGCACGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACca                       >  1:388417/1‑140 (MQ=255)                                                                                                           aCGTGGCGCGAAGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCCGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCt             >  2:231480/1‑140 (MQ=255)                                                                                                            cGTGGCGCGAAGCTGTCAATATCGTGGTTTATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTg            <  1:353919/140‑1 (MQ=255)                                                                                                                      aGCTGTCAATATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGag  <  1:231480/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                   CCAGGCATTGCGCTTTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAG  >  NC_000913/913505‑913761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑