Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	924,084	T→C	L274P (CTG→CCG)	clpA →	ATP‑dependent Clp protease ATP‑binding subunit ClpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	924,084	0	T	C	100.0%	62.9 / NA	19	L274P (CTG→CCG)	clpA	ATP‑dependent Clp protease ATP‑binding subunit ClpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/12);  total (7/12)

CGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCG  >  NC_000913/923951‑924172                                                                                                                                      |                                                                                         cGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTAAAAAACGTTTTGAAACGTTGCGCAAGCAGCCGGAGCa                                                                                    >  1:214790/1‑140 (MQ=255)  gAATTGTTCAGGGCGATGTGCCCGAGGTGTTGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAg                                                                                   <  1:277873/140‑1 (MQ=255)   aaTTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAgg                                                                                  <  1:588109/140‑1 (MQ=255)       gTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGacac                                                                              <  1:492030/140‑1 (MQ=255)                gATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCAt                                                                     <  1:593176/140‑1 (MQ=255)                  tgtgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGa                                                                                 <  2:732817/126‑1 (MQ=255)                  tgtgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGa                                                                                 >  1:732817/1‑126 (MQ=255)                  tgtgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATcc                                                                   >  1:517910/1‑140 (MQ=255)                   gtgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCt                                                                  <  1:567996/140‑1 (MQ=255)                         gAAGTGATGGCTGACTGGACGACTTACTCTCCCGATATCCGTTCTCTGCTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGTGCAGGACACTAACAGCATCCTGTTTAt                                                            <  2:233982/140‑1 (MQ=255)                              gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCATCCGGAGCCGGAGACTAACAGCATCCTGTTTATtgatg                                                       >  1:233982/1‑140 (MQ=255)                              gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCTGTTTATtgatg                                                       <  1:656766/140‑1 (MQ=255)                                    tGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATcc                                                 <  2:713227/140‑1 (MQ=255)                                     gACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCca                                                <  1:638009/140‑1 (MQ=255)                                                         cGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTg                            <  2:1633/140‑1 (MQ=255)                                                           atatCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGt                             <  2:374028/137‑1 (MQ=255)                                                           atatCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGt                             >  1:374028/1‑137 (MQ=255)                                                             atCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGc                        >  1:637216/1‑140 (MQ=255)                                                                                   caAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCCGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATTCACACCATCATCCGTGAGGGTGCAGAGTCTAGTGGTCAGGTCGATGCg  >  2:197865/1‑140 (MQ=255)                                                                                                                                      |                                                                                         CGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCG  >  NC_000913/923951‑924172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑