Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	993,073	C→T	R818C (CGT→TGT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	993,073	0	C	T	100.0%	90.2 / NA	27	R818C (CGT→TGT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

GCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCCGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAG  >  NC_000913/992936‑993210                                                                                                                                          |                                                                                                                                          gCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGt                                                                                                                                         <  2:166378/140‑1 (MQ=255)            aTGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGt                                                                                                                              <  2:528119/140‑1 (MQ=255)                 cAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTGAACAGCTGTAACCCGCAGGTGGCtt                                                                                                                         >  1:296851/1‑140 (MQ=255)                 cAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATCCTTACCGACCTAAAAAGCTGTGACCCGCAGGTGGCtt                                                                                                                         >  1:197346/1‑140 (MQ=255)                               ttcgttcgTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGaa                                                                                                           >  1:698751/1‑140 (MQ=255)                                                 cgTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGaaa                                                                                         >  1:387149/1‑140 (MQ=255)                                                       cGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACTCGCAGGTGGCTTCACGTCTGACTGAACCGCTGATTCGCCTGAAACGTTAc                                                                                   <  2:387149/140‑1 (MQ=255)                                                       cGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTAc                                                                                   <  2:658435/140‑1 (MQ=255)                                                                         cGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGgag                                                                 >  2:711997/1‑140 (MQ=255)                                                                           ttCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCCGATTCGCTAGAAACGTCACGATTCCAAACGCCAGGAGaa                                                               >  2:353174/1‑140 (MQ=255)                                                                                   cGAAGATGGCAGCGGTTACCTGCTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATgcgcg                                                       <  1:256343/140‑1 (MQ=255)                                                                                    gAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATgcgcgc                                                      <  1:85946/140‑1 (MQ=255)                                                                                          ggCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCt                                                >  2:33031/1‑140 (MQ=255)                                                                                          ggCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCt                                                >  1:91036/1‑140 (MQ=255)                                                                                            cAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTgg                                              >  2:610688/1‑140 (MQ=255)                                                                                            cAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTAAGATGCCAGACGGCACGAGAAAATGCGCGCGGCGCTgg                                              >  1:225783/1‑140 (MQ=255)                                                                                              gCGGTTACCTGTTCCTGCTGGATATGCTTACCGACTTCAGCAGCTGTACCCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATACCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGaa                                            <  2:197346/140‑1 (MQ=255)                                                                                                     ccTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGa                                     <  2:16391/140‑1 (MQ=255)                                                                                                         ttCCTGGTGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAgg                                 >  2:58287/1‑140 (MQ=255)                                                                                                         ttCCTGGGGGAAATGCTTACCGACCTCAACAGCTGTAACCCGCAGGGGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTTAGGAGAACATGTGCCCGGCGCGGGAACAGTTGAAAgg                                 >  1:281472/1‑140 (MQ=255)                                                                                                                   aaaTGCTTACCGACCTCCACAGCTGTAACCCGCAGTTGGTTTCACGTCGGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAAt                       <  2:296851/140‑1 (MQ=255)                                                                                                                   aaaTGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAAt                       >  1:469312/1‑140 (MQ=255)                                                                                                                    aaTGCTTACCGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATACGCGCGGGGCTCGATCAGTTGCAAGGGCTGGAAAAtc                      >  1:340004/1‑140 (MQ=255)                                                                                                                            ccGACCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAAtctctggcga              >  1:41307/1‑135 (MQ=255)                                                                                                                               aCCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGAt           <  1:610688/140‑1 (MQ=255)                                                                                                                               aCCTCAACAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGAt           <  1:58287/140‑1 (MQ=255)                                                                                                                                     aCAGCTGTAACCCGCACTTGGCGTCACGTCGGATTTAACCGCTGATTCTCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTAc     <  2:340004/140‑1 (MQ=255)                                                                                                                                      cAGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAA‑‑TCTCTGGCGATCTGTACgag  <  2:41307/140‑1 (MQ=255)                                                                                                                                       aGCTGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACga   <  2:91036/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                          GCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCCGTAACCCGCAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAG  >  NC_000913/992936‑993210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑