Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,015,783	A→G	G23G (GGA→GGG)	rmf →	ribosome modulation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,015,783	0	A	G	100.0%	161.8 / NA	46	G23G (GGA→GGG)	rmf	ribosome modulation factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)

CTGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTC  >  NC_000913/1015648‑1015907                                                                                                                                        |                                                                                                                             cTGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACTTCAGCGTGGTTATCAGGGCGGCATCGCCGGGCGCt                                                                                                                          >  2:242857/1‑140 (MQ=255)      aTTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCaaaa                                                                                                                     >  2:666781/1‑140 (MQ=255)      aTTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCaaaa                                                                                                                     >  1:620605/1‑140 (MQ=255)      aTTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCaaaa                                                                                                                     >  1:419539/1‑140 (MQ=255)          cacaTTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGaaa                                                                                                                 >  1:722486/1‑140 (MQ=255)           acaTTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGGACATCAACCTGGGTATAAGGCCGGCATCGCGGGGCGCTCAAAAGAAAt                                                                                                                >  2:320100/1‑140 (MQ=255)               ttAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATgtgt                                                                                                            <  2:693632/140‑1 (MQ=255)                   tAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCt                                                                                                        >  2:511013/1‑140 (MQ=255)                        aCTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAg                                                                                                   >  2:653840/1‑140 (MQ=255)                        aCTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAg                                                                                                   >  1:113029/1‑140 (MQ=255)                         cTGTTTTCTTTTCCACCAGGAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATgtgt                                                                                                            <  1:321976/130‑1 (MQ=255)                         cTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATgtgt                                                                                                            >  2:321976/1‑130 (MQ=255)                         cTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGa                                                                                                  <  1:561354/140‑1 (MQ=255)                         cTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGa                                                                                                  >  1:184718/1‑140 (MQ=255)                          tGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGACATCGCCAGGCGCTCAGAAGACATGTGTCCCTATCAGAc                                                                                                 >  2:319201/1‑140 (MQ=255)                            ttttcttttcCACCATAAACCACTATGAGCGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGc                                                                                               <  1:320100/140‑1 (MQ=255)                            ttttcttttcCACCAGAAACCAGTATGAGGGAAACGATGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGc                                                                                               <  1:358429/140‑1 (MQ=255)                               tcttttcCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCa                                                                                                    <  1:526632/132‑1 (MQ=255)                               tcttttcCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCa                                                                                                    >  2:526632/1‑132 (MQ=255)                                 ttttcCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAAt                                                                                          >  2:559292/1‑140 (MQ=255)                                      caccaGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCAACGCCGGGCACTCGAAAGAAATGTGTGCCTAGCAGACGCTGAATCAAAg                                                                                     >  2:212605/1‑140 (MQ=255)                                            aaaCCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTcaca                                                                               <  2:2876/140‑1 (MQ=255)                                                        gggAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAgg                                                                   >  2:575428/1‑140 (MQ=255)                                                          gAAACGAGGCATGAACAGACAATAAGGAGGTCGCCTGAAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCt                                                                 <  1:252829/140‑1 (MQ=255)                                                            aaCGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTgg                                                               <  2:184718/140‑1 (MQ=255)                                                                        agagACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATg                                                   <  1:511013/140‑1 (MQ=255)                                                                        agagACAAAAACGAGATCGCCTCGAACGGGAACCTAAACATGGTTATCCGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATg                                                   <  2:338013/140‑1 (MQ=255)                                                                            aCAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGCTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCgaga                                                          <  2:170751/129‑1 (MQ=255)                                                                            aCAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGCTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCgaga                                                          >  1:170751/1‑129 (MQ=255)                                                                             cAAAAACGAGATCGCGTGGAACCGGCACATCAACGTGATTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGa                                              <  1:196750/140‑1 (MQ=255)                                                                             cAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGa                                              <  2:483001/140‑1 (MQ=255)                                                                             cAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGa                                              <  1:717302/140‑1 (MQ=255)                                                                             cAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGa                                              <  2:130288/140‑1 (MQ=255)                                                                             cAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGa                                              <  1:653840/140‑1 (MQ=255)                                                                             cAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGa                                              <  1:460523/140‑1 (MQ=255)                                                                             cAAAAACGAGATCGCCTGGAACAGGCACAACAACGTGATTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGa                                              <  1:242857/140‑1 (MQ=255)                                                                              aaaaaCGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGAc                                             <  1:575428/140‑1 (MQ=255)                                                                                  aCGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAggg                                         <  1:559292/140‑1 (MQ=255)                                                                                              ggAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCt                             >  1:183851/1‑140 (MQ=255)                                                                                               gAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTg                            >  1:601917/1‑140 (MQ=255)                                                                                                    ggCACATCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCt                       >  2:471406/1‑140 (MQ=255)                                                                                                      cacaTCAACGTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGt                     >  1:677804/1‑140 (MQ=255)                                                                                                               gTGGTTATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTaaa            <  2:620605/140‑1 (MQ=255)                                                                                                                   ttATCAGGCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa        >  1:69428/1‑140 (MQ=255)                                                                                                                         ggCCGGCATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTc  >  2:546209/1‑140 (MQ=255)                                                                                                                               cATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCt   >  2:115134/1‑133 (MQ=255)                                                                                                                               cATCGCCGGGCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCt   <  1:115134/133‑1 (MQ=255)                                                                                                                                        |                                                                                                                             CTGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTC  >  NC_000913/1015648‑1015907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑