Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,103,670	C→T	intergenic (‑474/‑281)	csgD ← / → csgB	DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,103,670	0	C	T	100.0%	130.6 / NA	41	intergenic (‑474/‑281)	csgD/csgB	DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/23);  total (18/23)

AAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAA  >  NC_000913/1103534‑1103804                                                                                                                                         |                                                                                                                                       aaaaaTATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAATACGTATCGCTGCAATCAGCGATggg                                                                                                                                     >  1:348187/1‑140 (MQ=255)   aaaTATTTCGGTGTATTCCTTTAGTCATGTAAAACGTCCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCa                                                                                                                                   <  1:212744/140‑1 (MQ=255)      tatTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCaaga                                                                                                                                >  1:695880/1‑140 (MQ=255)         ttCGGTGTAGTCCTTTCGACATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAagaaga                                                                                                                             >  1:719420/1‑140 (MQ=255)             gtgtAGTCCTTTCGTTATGTAATACCTTCATGTTTTTACTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGa                                                                                                                         <  1:284846/140‑1 (MQ=255)                tAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTg                                                                                                                      >  2:622187/1‑140 (MQ=255)                tAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAACAACCACCGATGGTCAAGAAGAATGACTg                                                                                                                      >  1:247815/1‑140 (MQ=255)                   tCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCt                                                                                                                   >  1:65882/1‑140 (MQ=255)                     cTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAATAAGAATGACTGTCTgg                                                                                                                 <  2:40822/140‑1 (MQ=255)                     cTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAATAAGAATGACTGTCTgg                                                                                                                 >  1:40822/1‑140 (MQ=255)                     cTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTgg                                                                                                                 <  2:445436/140‑1 (MQ=255)                             aTGTAAAACGTTCTTGTTTTTTCTCCACACTTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTgg                                                                                                                 >  2:457975/1‑132 (MQ=255)                             aTGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTgg                                                                                                                 <  1:457975/132‑1 (MQ=255)                             aTGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCttttt                                                                                                         >  1:314668/1‑140 (MQ=255)                               gTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGa                                                                                                       <  2:682942/140‑1 (MQ=255)                                 aaaaCGTTCTTGTTTTTTCTCCCCACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATa                                                                                                     <  2:314668/140‑1 (MQ=255)                                      gTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGa                                                                                                <  2:681752/140‑1 (MQ=255)                                         cTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCGGCGATGGGCAAGGAGATTGACTGTCTGGTGCTTTTTGATAGCGGaaaa                                                                                             >  2:245928/1‑140 (MQ=255)                                         cTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaaa                                                                                             >  1:685069/1‑140 (MQ=255)                                             ttttttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACAAATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGga                                                                                         >  2:708942/1‑140 (MQ=255)                                                      cacaCCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGGCTGGTGCTTTTTGACAGCGGACAACGGAGATTTaaaa                                                                                >  1:296238/1‑140 (MQ=255)                                                             ccGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACa                                                                         >  1:90186/1‑140 (MQ=255)                                                                tGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACaaaa                                                                      >  1:179327/1‑140 (MQ=255)                                                                tGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACaaaa                                                                      >  2:367515/1‑140 (MQ=255)                                                                   aCAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAAACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGGGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAatat                                                                   >  2:160101/1‑140 (MQ=255)                                                                         ttttACTGCAAAAAGTCGAGGTTTGCCACCGCTTGTGTGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATAttttttt                                                             <  1:202330/140‑1 (MQ=255)                                                                         ttttACTGCAAAAAGTCGAGGTCTGACACCGCTTGTGGGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATAttttttt                                                             <  1:219062/140‑1 (MQ=255)                                                                         ttttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATAttttttt                                                             <  1:103207/140‑1 (MQ=255)                                                                         ttttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATAttttttt                                                             <  2:90186/140‑1 (MQ=255)                                                                           ttACTGCAAAAAGACGGTGTTTGTCACGGCATGTGCGCAAGACATATCTCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGc                                                           <  2:348187/140‑1 (MQ=255)                                                                                 cAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGAt                                                     <  2:685069/140‑1 (MQ=255)                                                                                   aaaaGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATaa                                                   <  2:719420/140‑1 (MQ=255)                                                                                   aaaaGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATaa                                                   <  1:370450/140‑1 (MQ=255)                                                                                     aaGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACa                                                 <  1:719731/140‑1 (MQ=255)                                                                                     aaGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACa                                                 <  1:622187/140‑1 (MQ=255)                                                                                     aaGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACa                                                 <  1:367515/140‑1 (MQ=255)                                                                                                  tCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACAGCGTATTTACGTg                                    <  2:695880/140‑1 (MQ=255)                                                                                                          tgtgCGCAAGACATATTACAGCTATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACAGCGTATTTACGTGGGTTTTaa                            <  2:328587/140‑1 (MQ=255)                                                                                                                             agcaATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTa                  <  2:463338/131‑1 (MQ=255)                                                                                                                             agcaATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTa                  >  1:463338/1‑131 (MQ=255)                                                                                                                             agcaATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGGAGATAACAACGTATTTGCGTGGGTTTTAATACTTTGGTATGAACTaaa         >  2:310192/1‑140 (MQ=255)                                                                                                                              gcaATCAGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTaaaa        <  1:160101/140‑1 (MQ=255)                                                                                                                                    aGCGATGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGaaa  >  2:28118/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTTTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAA  >  NC_000913/1103534‑1103804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑