Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,463	A→G	M118V (ATG→GTG)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,463	0	A	G	100.0%	134.0 / NA	38	M118V (ATG→GTG)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/20);  total (18/20)

CTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGATGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCGATGATAAGTACGCCACCGTAGCGCAGGCACTTGAACTGGAAAACCCG  >  NC_000913/1154328‑1154573                                                                                                                                        |                                                                                                               ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACT‑GTAGAAGGGGTGCGt                                                                                                           >  1:481788/1‑140 (MQ=255)     gTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTg                                                                                                        <  1:438018/140‑1 (MQ=255)          gAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGa                                                                                                   <  1:632200/140‑1 (MQ=255)             ccGGATCTTTCTAACTTTAAAGCCGGGACTTACCGTTTTCCACCGCAGCTGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTa                                                                                                <  2:341360/140‑1 (MQ=255)                gATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTg                                                                                                        <  2:726762/129‑1 (MQ=255)                gATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTg                                                                                                        >  1:726762/1‑129 (MQ=255)                       ctcACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCaa                                                                                      <  2:26126/140‑1 (MQ=255)                       ctcACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATACTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCaa                                                                                      >  2:160477/1‑140 (MQ=255)                             ttAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGt                                                                                >  2:145112/1‑140 (MQ=255)                             ttAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACT‑GTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTg                                                                               <  2:481788/140‑1 (MQ=255)                              tAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTg                                                                               >  2:544911/1‑140 (MQ=255)                                aaGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTAcc                                                                                              <  1:106100/123‑1 (MQ=255)                                aaGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTAcc                                                                                              >  2:106100/1‑123 (MQ=255)                                aaGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGa                                                                              <  2:570969/139‑1 (MQ=255)                                aaGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGa                                                                              >  1:570969/1‑139 (MQ=255)                                aaGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAg                                                                             <  1:152747/140‑1 (MQ=255)                                aaGCCGGGACTTACCGCCTTACCCCGCAGATGACCATGCGCGAGATGCGGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAg                                                                             <  2:311347/140‑1 (MQ=255)                                 aGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAATTTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAgg                                                                            <  1:384519/140‑1 (MQ=255)                                  gCCGGGACTTACCGCCTTACACCGCTGATTACCGTGCCCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGc                                                                           <  1:235190/140‑1 (MQ=255)                                    cGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGccc                                                                         >  2:531641/1‑140 (MQ=255)                                    cGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGccc                                                                         >  2:375486/1‑140 (MQ=255)                                    cGGGACTTACCGCTTTACACCGAAGATGACCTTGCGCGAGATGCTGAAGTTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGccc                                                                         <  2:288987/140‑1 (MQ=255)                                      ggACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCTATTGCCTCAAGCACTTGCGTGAGGCCCCg                                                                       >  1:200082/1‑140 (MQ=255)                                      ggACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCg                                                                       >  2:674634/1‑140 (MQ=255)                                           tACCGCTTTACACCGCAGATGACCGTGCGCGAGATACTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTatat                                                                  <  1:160477/140‑1 (MQ=255)                                                  ttACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCAt                                                           >  2:408162/1‑140 (MQ=255)                                                         gCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCGCAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGa                                                    <  2:508252/140‑1 (MQ=255)                                                          cAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAg                                                   >  1:615138/1‑140 (MQ=255)                                                               gACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCgatg                                              <  1:375486/140‑1 (MQ=255)                                                               gACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCgatg                                              <  1:531641/140‑1 (MQ=255)                                                                  cGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCGATGATa                                           >  1:131114/1‑140 (MQ=255)                                                                    tGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCGATGATAAg                                         >  2:403814/1‑140 (MQ=255)                                                                    tGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCAATGATAAg                                         <  1:145112/140‑1 (MQ=255)                                                                                tGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCCAGCGTACGCTGAGCGATGATAAGTACGCCACCGTa                             >  2:283431/1‑140 (MQ=255)                                                                                 gAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGTGTGAGGCCCCGTATATCAAGCATATGCTGAGCGATGATAAGTACGCCACCGTAg                            <  1:559357/140‑1 (MQ=255)                                                                                                     aagaagCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCGATGATAAGTACGCCACCGTAGCGCAGGCACTTGAACTGGaa        >  2:492743/1‑140 (MQ=255)                                                                                                          gCACAGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCGATGATAAGTACGCCACCGTAGCGCAGGCACTTGAACTGGAAAAccc   >  2:481914/1‑140 (MQ=255)                                                                                                           cacaGTTCCCTCTGCGACTGGTAGAAGGGGTGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCGATGATAAGTACGCCACCGTAGCGCAGGCACTTGAACTGGAAAACCCg  <  1:544911/140‑1 (MQ=255)                                                                                                                                        |                                                                                                               CTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTGAAATTGCTGGAAAGCGGTAAAGAAGCACAGTTCCCTCTGCGACTGGTAGAAGGGATGCGTCTGAGCGATTACCTCAAGCAATTGCGTGAGGCCCCGTATATCAAGCATACGCTGAGCGATGATAAGTACGCCACCGTAGCGCAGGCACTTGAACTGGAAAACCCG  >  NC_000913/1154328‑1154573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑