Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,369	T→C	Q728R (CAA→CGA)	fhuE ←	ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,369	0	T	C	100.0%	79.1 / NA	23	Q728R (CAA→CGA)	fhuE	ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/12);  total (11/12)

TCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGG  >  NC_000913/1159233‑1159497                                                                                                                                         |                                                                                                                                 tCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGAt                                                                                                                               <  2:571834/140‑1 (MQ=255)                          gATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTAcg                                                                                                      >  2:720422/1‑140 (MQ=255)                          gATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTAcg                                                                                                      <  2:416513/140‑1 (MQ=255)                          gATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTAcg                                                                                                      <  2:390033/140‑1 (MQ=255)                            tAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTAcgcg                                                                                                    <  1:13395/140‑1 (MQ=255)                                 tGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGtgtg                                                                                               <  2:550089/140‑1 (MQ=255)                                          agaTGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACg                                                                                      >  1:564304/1‑140 (MQ=255)                                               gatgAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGa                                                                                 <  1:720422/140‑1 (MQ=255)                                                     tACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTc                                                                           >  1:630764/1‑140 (MQ=255)                                                      aCATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTcc                                                                          <  2:564304/140‑1 (MQ=255)                                                        aTCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTGCGCGGTGTGCCGTAGACGATAGAACCTTCCAc                                                                        <  2:74751/140‑1 (MQ=255)                                                            gTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTg                                                                    <  1:377059/140‑1 (MQ=255)                                                                    tAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAGATTACGCGGTGTTCCGTCGACGATAGAACCCACCACGTTAGTATCGTa                                                            >  2:338692/1‑140 (MQ=255)                                                                             aGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTc                                                   <  2:701782/140‑1 (MQ=255)                                                                                cGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGaa                                                >  2:394554/1‑140 (MQ=255)                                                                                        agaCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTAt                                        <  2:630764/140‑1 (MQ=255)                                                                                           cTAAGGCAGCCAGATCGCTGCCATTATTATAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGa                                     <  1:338692/140‑1 (MQ=255)                                                                                               ggCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGtt                                 <  2:598394/140‑1 (MQ=255)                                                                                                  aGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGATATCGTAGGTTGTGACGAACACGTTATTGACGTTccc                              >  1:338847/1‑140 (MQ=255)                                                                                                    ccAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCt                            >  2:546294/1‑140 (MQ=255)                                                                                                             tGCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACgaga                   >  1:88703/1‑140 (MQ=255)                                                                                                              gCCTTTTTTACAGGTGTTATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGaa                  >  1:255315/1‑140 (MQ=255)                                                                                                                              ttATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTgg  >  1:643522/1‑140 (MQ=255)                                                                                                                              ttATTCAGAATCGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTCGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTACCCCTGTATCGAGGAGTTTTTCGTCACCTgg  >  2:212325/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                 TCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGG  >  NC_000913/1159233‑1159497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑