Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,348	A→G	S422G (AGT→GGT)	ndh →	NADH:quinone oxidoreductase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,348	0	A	G	100.0%	139.6 / NA	40	S422G (AGT→GGT)	ndh	NADH:quinone oxidoreductase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/15);  total (25/15)

CCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGCCGCGTTGTATCAGGCATCCTTTCAGA  >  NC_000913/1167212‑1167482                                                                                                                                         |                                                                                                                                       ccTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTa                                                                                                                                     >  1:525810/1‑140 (MQ=255)  cTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTAt                                                                                                                                    >  1:389704/1‑140 (MQ=255)  cTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTAt                                                                                                                                    <  1:634691/140‑1 (MQ=255)     aTGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTaa                                                                                                                                 >  1:595038/1‑140 (MQ=255)      tGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAAc                                                                                                                                <  2:70132/140‑1 (MQ=255)               tGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTAtc                                                                                                                       >  1:549700/1‑140 (MQ=255)                   gcgcgGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTAtccgtc                                                                                                                   >  2:590214/1‑140 (MQ=255)                   gcgcgGCTCAATGATGATTGAACGATGAATTGCGCGCGTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTAtccgtc                                                                                                                   <  1:249082/140‑1 (MQ=255)                       ggCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCcgcg                                                                                                               >  2:649951/1‑140 (MQ=255)                                   atTGAAGGACGAATTGCGCGCTTTGTATATATGTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGtt                                                                                                             >  2:502080/1‑130 (MQ=255)                                   atTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAAGCGCGTTGTCCGTCAGCGTTTGAAGTTGCa                                                                                                   >  1:272515/1‑140 (MQ=255)                                   atTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGtt                                                                                                             <  1:502080/130‑1 (MQ=255)                                       aaGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTaa                                                                                               <  2:595038/140‑1 (MQ=255)                                       aaGGACGAATTGCGCGCGTTGTATATTGCTCGCTATACCGAATGCATCAGGTTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTaa                                                                                               <  2:7610/140‑1 (MQ=255)                                          gACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCg                                                                                            <  2:128337/140‑1 (MQ=255)                                               aTTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTa                                                                                       >  1:688523/1‑140 (MQ=255)                                               aTTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTa                                                                                       <  2:525810/140‑1 (MQ=255)                                                 tGCGCGCTTTGTATATAACTCGATATACCGAATGCCTCATATTGCGCTTCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCATTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTaca                                                                                     <  2:224999/140‑1 (MQ=255)                                                                  tctcGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATa                                                                    <  2:549700/140‑1 (MQ=255)                                                                    tcGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGCATTAACCGCGTTATCCGTCCGCGTTTGAAGGTGCATTACTCGGCGGACACTGTCGGATTTGGCATaaa                                                                  >  1:233991/1‑140 (MQ=255)                                                                      gCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACg                                                                >  1:87118/1‑140 (MQ=255)                                                                      gCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACg                                                                >  1:145103/1‑140 (MQ=255)                                                                      gCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGGTGCGTTAATCGACGTAAACTGTCGGATGTGGCATAAACg                                                                >  2:296816/1‑140 (MQ=255)                                                                             cGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATc                                                         <  2:688523/140‑1 (MQ=255)                                                                                aTGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGc                                                      <  2:389704/140‑1 (MQ=255)                                                                                aTGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGc                                                      >  1:625945/1‑140 (MQ=255)                                                                                aTGCATCACATTGCGGTCCATGCTTACTTTATATCTGGATTAATGATGCTGGGGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGc                                                      <  2:358124/140‑1 (MQ=255)                                                                                    aTCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGACATACACGCCTCATCCGCCCtt                                                  >  2:201075/1‑140 (MQ=255)                                                                                         aTTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTAGCGGATGTGGCATAGACGCCTTATCCGCCCTTGAGGa                                             >  1:213741/1‑140 (MQ=255)                                                                                           tGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAAc                                           >  1:705249/1‑140 (MQ=255)                                                                                            gcgcTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACa                                          >  2:25019/1‑140 (MQ=255)                                                                                             cgcTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAg                                         <  2:705249/140‑1 (MQ=255)                                                                                             cgcTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGACGCGGCATAAACGCCTCAGCCGCCCTTGAGGAACAg                                         >  2:340697/1‑140 (MQ=255)                                                                                             cgcTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACCTACACTGGAGGATGAGGCATAAACGTCTCACCCGCCCTTGAGGAACAg                                         >  2:217714/1‑140 (MQ=255)                                                                                              gcTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAgc                                        >  1:93345/1‑140 (MQ=255)                                                                                                          cTTTAAAACCGGATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGc                            <  2:145103/140‑1 (MQ=255)                                                                                                                     gATTAATGATGCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAAAGCCTCATCTGCCTTCGTGGAACAGCACGATCGGCAGCCGCGTTGTATc                 >  2:324955/1‑140 (MQ=255)                                                                                                                           tgatgCTGGTGGGGGCTATTAATCGAGTTATCCGTCCCCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGCCGCGTTGTATCAGGCAt           <  1:324955/140‑1 (MQ=255)                                                                                                                               gCTGGTGGGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGCCGCGTTGTATCAGGCATCCtt       >  2:183747/1‑140 (MQ=255)                                                                                                                                   gtggGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGCCGCGTTGTATCAGGCATCCTTTCAg   >  1:425408/1‑140 (MQ=255)                                                                                                                                    tggGGGGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGCCGCGTTGTATCAGGCATCCTTTCAGa  >  1:180066/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGCCGCGTTGTATCAGGCATCCTTTCAGA  >  NC_000913/1167212‑1167482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑