Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,231,808	A→G	D208G (GAT→GGT)	umuC →	DNA polymerase V catalytic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,231,808	0	A	G	100.0%	41.6 / NA	13	D208G (GAT→GGT)	umuC	DNA polymerase V catalytic protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/8);  total (5/8)

TGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTT  >  NC_000913/1231676‑1231944                                                                                                                                     |                                                                                                                                         tggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCCATGTGGAACAAAACCGTTCTCGTTTTGGCGGGTAAAGat                                                                                                                                   >  2:331042/1‑140 (MQ=255)        aTCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGt                                                                                                                            >  1:98188/1‑140 (MQ=255)                   aaCGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTaaa                                                                                                                 <  2:77453/140‑1 (MQ=255)                            gTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTAAACATTTTAAt                                                                                                        >  2:152757/1‑140 (MQ=255)                                    aTGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCt                                                                                                <  2:98188/140‑1 (MQ=255)                                     tGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTaaa                                                                                                                 <  1:57970/122‑1 (MQ=255)                                     tGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTaaa                                                                                                                 >  2:57970/1‑122 (MQ=255)                                           ctctCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGa                                                                                         >  1:118123/1‑140 (MQ=255)                                                cccGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGGAAAGAAATCCGGTTTTTCCCTAAAAATTTTAATGTTGTGCTCGAAAAAACggg                                                                                    >  2:707735/1‑139 (MQ=255)                                                           cGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTgc                                                                         <  1:707735/140‑1 (MQ=255)                                                               tGGGGGATTGGACGGGGGATCAGCAAAAATCTGGACGGGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCgcgg                                                                     <  1:331042/140‑1 (MQ=255)                                                                   ggATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGaa                                                                 <  1:152757/140‑1 (MQ=255)                                                                      ttGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGGACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAAccc                                                              >  2:512975/1‑140 (MQ=255)                                                                               ggATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCaa                                                     >  1:517557/1‑140 (MQ=255)                                                                                        aaaaaCTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGGGACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCTTGAACTGGGCGGCGGACCCTGGTTGCAACTGGAagag                                            >  1:271266/1‑140 (MQ=255)                                                                                                        ggggATCAAAACCGTTCTCGATTTGGCGGGTACAGATATCCGGTATATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGc                            <  2:422406/140‑1 (MQ=255)                                                                                                                           gATTTGGCGGGTGCAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCg         <  2:411975/140‑1 (MQ=255)                                                                                                                                  cGGGTACAGATATCCGGTTTATCCGTAAACACTTTAATGCCGTGCTCGGAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGttt  <  2:271266/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTT  >  NC_000913/1231676‑1231944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑