Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,112	G→A	intergenic (+163/‑148)	dadX → / → ymgM	alanine racemase 2/protein YmgM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,112	0	G	A	100.0%	104.6 / NA	32	intergenic (+163/‑148)	dadX/ymgM	alanine racemase 2/protein YmgM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

CGTCTTCTGGGTGCAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAAT  >  NC_000913/1239976‑1240247                                                                                                                                         |                                                                                                                                        cGTCTTCTGGGTGCAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGt                                                                                                                                      <  1:380071/140‑1 (MQ=255)  gTCTTCTGGGTGCAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGtt                                                                                                                                     <  1:250306/140‑1 (MQ=255)            tGCAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGAAACTATTTCCCCGGAACCTGAACTCATCGCACAGACGTTCTACATaaaa                                                                                                                           >  2:306233/1‑140 (MQ=255)              cAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAacg                                                                                                                         >  1:659569/1‑140 (MQ=255)              cAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAacg                                                                                                                         >  1:427363/1‑140 (MQ=255)               aaaaaaaTCATCCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCAGCGGACAGACGTTCTACATAAAacgc                                                                                                                        >  1:196564/1‑140 (MQ=255)                                             cTGTAGTTGTTAATGTGACAGAGCCGTTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACt                                                                                          <  1:306233/140‑1 (MQ=255)                                                           gtgACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACg                                                                            <  1:652069/140‑1 (MQ=255)                                                             gACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCt                                                                          >  2:540528/1‑140 (MQ=255)                                                             gACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCCGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCt                                                                          <  2:424429/140‑1 (MQ=255)                                                                    ccATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCt                                                                   >  1:475020/1‑140 (MQ=255)                                                                     cATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACGCGAACTCGACTTGAGATGAATCACGCTTTACCCtt                                                                  >  2:191953/1‑140 (MQ=255)                                                                        tGCCCATGATAGTGTCCATTAACAGGATGGACACTCTTTCCCCGGAACTTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGAt                                                               <  2:196564/140‑1 (MQ=255)                                                                             aTGATAGTGTCCATTCAAAGGATGGTCACGATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGc                                                          <  1:307410/140‑1 (MQ=255)                                                                             aTGATAGTGTCCATTAAAAGGATGGTCACGATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTCCCCTTGATGGAGc                                                          <  1:288020/140‑1 (MQ=255)                                                                             aTGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGc                                                          <  2:475020/140‑1 (MQ=255)                                                                             aTGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTATTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGc                                                          <  2:427363/140‑1 (MQ=255)                                                                                    tgtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGc                                                          >  2:576893/1‑133 (MQ=255)                                                                                    tgtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGc                                                          <  1:576893/133‑1 (MQ=255)                                                                                    tgtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCt                                                        <  1:527102/135‑1 (MQ=255)                                                                                    tgtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCt                                                        >  2:527102/1‑135 (MQ=255)                                                                                    tgtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACa                                                   >  1:553261/1‑140 (MQ=255)                                                                                        cATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGc                                                          >  1:461376/1‑129 (MQ=255)                                                                                        cATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGc                                                          <  2:461376/129‑1 (MQ=255)                                                                                         aTTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGAt                                              >  1:619105/1‑140 (MQ=255)                                                                                           tAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGAttt                                            >  2:503765/1‑140 (MQ=255)                                                                                                    ggACACTATTTCCCCGGGACCTTAACTCACCCCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATC‑CGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGc                                  <  2:282590/140‑1 (MQ=255)                                                                                                      acacTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGcc                                 <  2:659569/140‑1 (MQ=255)                                                                                                                  cGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGatat                     >  1:553209/1‑140 (MQ=255)                                                                                                                                aCCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGat                       >  2:497741/1‑124 (MQ=255)                                                                                                                                aCCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGat                       <  1:497741/124‑1 (MQ=255)                                                                                                                                     acaGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAAt  <  1:540528/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGTCTTCTGGGTGCAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAAT  >  NC_000913/1239976‑1240247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑