Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	152,110	A→G	V41A (GTA→GCA)	yadL ←	putative fimbrial protein YadL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	152,110	0	A	G	100.0%	263.5 / NA	73	V41A (GTA→GCA)	yadL	putative fimbrial protein YadL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/38);  total (35/38)

TCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTT  >  NC_000913/151975‑152244                                                                                                                                        |                                                                                                                                       tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCagt                                                                                                                                    >  1:520719/1‑140 (MQ=255)   tttGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCagtag                                                                                                                                  >  1:465205/1‑140 (MQ=255)    ttGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTAAAAGTACCCATTTCTGCagtagt                                                                                                                                 >  1:264498/1‑140 (MQ=255)           tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACg                                                                                                                          >  1:107869/1‑140 (MQ=255)           tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACg                                                                                                                          <  2:633354/140‑1 (MQ=255)           tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACg                                                                                                                          >  1:501991/1‑140 (MQ=255)             aGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGt                                                                                                                        <  2:701477/140‑1 (MQ=255)             aGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGt                                                                                                                        <  1:56487/140‑1 (MQ=255)             aGTTTGAATGTTTTTACTTTGGCCTTGGCATCGATTTCATAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGt                                                                                                                        <  2:264498/140‑1 (MQ=255)                       tttttACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATa                                                                                                              <  2:501991/140‑1 (MQ=255)                       ttcttACTTTGCTCTGAGCATGGACTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGACCAGAACCATTTACTAATGTAGCGGTACAAGTACCCATCTCTGCAGTCGTAATAACGGTCAGTCCAATa                                                                                                              <  2:457847/137‑1 (MQ=255)                            aCTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATActgtc                                                                                                         >  1:81068/1‑140 (MQ=255)                               ttGGTCTTGGCATTGATTTCAGAAATATATACATCACCACAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATActgtctgt                                                                                                      <  2:332213/140‑1 (MQ=255)                               ttGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAGCGGTCAGTCCAATActgtctgt                                                                                                      >  1:279490/1‑140 (MQ=255)                               ttGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATActgtctgt                                                                                                      <  2:81068/140‑1 (MQ=255)                                 ggTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTg                                                                                                    <  2:534633/140‑1 (MQ=255)                                 ggTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGAACCCATTTCTGCAGTAGAAATAACGGTAAGTCCAATACTGTCTGTTg                                                                                                    >  1:95607/1‑140 (MQ=255)                                   tCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGcc                                                                                                  <  2:107869/140‑1 (MQ=255)                                       ggCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCAt                                                                                              >  1:48778/1‑140 (MQ=255)                                            tGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTg                                                                                                    <  1:11767/129‑1 (MQ=255)                                            tGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTg                                                                                                    >  2:11767/1‑129 (MQ=255)                                            tGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagag                                                                                         >  2:520495/1‑140 (MQ=255)                                            tGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagag                                                                                         >  2:570590/1‑140 (MQ=255)                                             gATTTCAGAAATATATATATCACCAAAATCACCAAGAGATATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagaga                                                                                        <  2:233119/140‑1 (MQ=255)                                                   aGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagaga                                                                                        <  2:174400/134‑1 (MQ=255)                                                   aGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagaga                                                                                        >  1:174400/1‑134 (MQ=255)                                                               aTCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTAGCCGCATAGGAGATTACGCTGAACAATGAgg                                                                      >  2:283237/1‑140 (MQ=255)                                                                 cACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCa                                                                    >  2:572436/1‑140 (MQ=255)                                                                 cACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCa                                                                    >  2:510579/1‑140 (MQ=255)                                                                 cACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGGCCAATACTATCTCTTGACGCATAAGAGAGTTCGCTGAACAATGAGGCa                                                                    >  2:253169/1‑140 (MQ=255)                                                                    cAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCGATACTGTCGGTTGCCGAATAAGAGAGTACGATGAACAATGAGGCAGcc                                                                 >  2:201826/1‑140 (MQ=255)                                                                    cAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTTCCGAATAAGAGAGTACGGTGAACAATGAGGCAGcc                                                                 >  2:242553/1‑140 (MQ=255)                                                                      aaaTCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCaa                                                               >  2:460314/1‑140 (MQ=255)                                                                         tCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAAc                                                            >  2:401555/1‑140 (MQ=255)                                                                           aacaacAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGa                                                          <  1:694862/140‑1 (MQ=255)                                                                           aacaacAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGa                                                          <  1:731086/140‑1 (MQ=255)                                                                           aacaacAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGa                                                          <  1:481253/140‑1 (MQ=255)                                                                           aacaacAGAAATGTCCTGATCAGAGTCATCTACTAATCTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGa                                                          <  1:348836/140‑1 (MQ=255)                                                                             caacaGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGaca                                                        <  2:465205/140‑1 (MQ=255)                                                                               acaGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAG‑CAAAACGACActg                                                     <  1:520495/140‑1 (MQ=255)                                                                                caGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACActg                                                     <  2:25159/140‑1 (MQ=255)                                                                                caGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACActg                                                     <  2:59440/140‑1 (MQ=255)                                                                                caGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACActg                                                     >  2:112938/1‑140 (MQ=255)                                                                                caGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACActg                                                     <  1:570590/140‑1 (MQ=255)                                                                                caGAAATGTCCTGATCAGAGTCATTTACTAAGGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACActg                                                     <  2:292177/140‑1 (MQ=255)                                                                                   aaaTGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCAGCATAAGAAAGTGCGCGGAACAATGGGGCATACAAAACGACActgctg                                                  >  2:348836/1‑140 (MQ=255)                                                                                     aggTCCTGATCAGAGTCTTTTACTAATGTAGCGGTCCAAGTACCCATTCCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGa                                                <  2:293381/138‑1 (MQ=255)                                                                                     aTGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGa                                                <  1:460314/140‑1 (MQ=255)                                                                                        tCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATaa                                             >  2:494895/1‑140 (MQ=255)                                                                                        tCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATaa                                             >  2:637111/1‑140 (MQ=255)                                                                                                  agTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGGGGCAGCCAAAATGAAACTGCTGGATAATCCATAAcct                                   >  1:293381/1‑138 (MQ=255)                                                                                                  agTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGt                                   >  1:699909/1‑140 (MQ=255)                                                                                                  agTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTACCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACActg                                                     >  1:430336/1‑122 (MQ=255)                                                                                                  agTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTACCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACActg                                                     <  2:430336/122‑1 (MQ=255)                                                                                                    tCATTTACTAATGTATCGGTACAAGTACCCATTTCTGTAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTaa                                 <  1:242553/140‑1 (MQ=255)                                                                                                    tCATTTACTAATGTAACGGTACTAGCACCCATTTCTGTAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTaa                                 <  1:201826/140‑1 (MQ=255)                                                                                                            tAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTaa                         >  1:146349/1‑140 (MQ=255)                                                                                                              aTGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAAt                       <  1:401555/140‑1 (MQ=255)                                                                                                                  aGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtca                   <  1:510579/140‑1 (MQ=255)                                                                                                                   gCGGTACAAGTACCCCTTTCTGCAGTAGTCATAACGGGCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtcat                  <  1:253169/140‑1 (MQ=255)                                                                                                                   gCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtcat                  <  2:95607/140‑1 (MQ=255)                                                                                                                      gTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCACAACGACACTGCTCGATAATCCATAACGTAAATTTTTAAATGTcatcat               >  2:222277/1‑140 (MQ=255)                                                                                                                      gTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTcatcat               <  1:112938/140‑1 (MQ=255)                                                                                                                        aCAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtcat                  >  2:152521/1‑135 (MQ=255)                                                                                                                        aCAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtcat                  <  1:727666/135‑1 (MQ=255)                                                                                                                        aCAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtcat                  >  2:727666/1‑135 (MQ=255)                                                                                                                        aCAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtcat                  <  1:152521/135‑1 (MQ=255)                                                                                                                              aCCCATTTCTGCAGTAGAAATAACGGTCAGTCCAATACTATCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATcc       <  2:279490/140‑1 (MQ=255)                                                                                                                                ccATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCctt     <  2:520719/140‑1 (MQ=255)                                                                                                                                 cATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCGAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCcttc    >  1:689111/1‑140 (MQ=255)                                                                                                                                 cATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtca                   <  2:470702/125‑1 (MQ=255)                                                                                                                                 cATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGtca                   >  1:470702/1‑125 (MQ=255)                                                                                                                                   tttCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCcttctt  >  2:376680/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTT  >  NC_000913/151975‑152244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑