Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,528	C→T	G515D (GGC→GAC)	cvrA ←	putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,528	0	C	T	100.0%	127.4 / NA	39	G515D (GGC→GAC)	cvrA	putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/20);  total (19/20)

CTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAC  >  NC_000913/1240391‑1240660                                                                                                                                          |                                                                                                                                     cttcttTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCAGCTTATCACGATATTCTAGCCCGTCTTCCAGGTCa                                                                                                                                    >  2:293531/1‑140 (MQ=255)   tctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCata                                                                                                                                  >  1:515321/1‑140 (MQ=255)     tttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCTGCGTTTGCAGCTGATCGCGATATTCGCGCCTGTCTTCCAGGTCatata                                                                                                                                >  2:210111/1‑140 (MQ=255)     tttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCatata                                                                                                                                >  2:118266/1‑140 (MQ=255)      ttctCGGCCACCGTCCAGATCAACCTGGCCAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCatatat                                                                                                                               <  1:210111/140‑1 (MQ=255)                     cAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGc                                                                                                                <  2:698231/140‑1 (MQ=255)                         tcatcCCGGCAAACTTTACCTGGTCACCGTCAACCGGAGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCacat                                                                                                            <  1:236301/140‑1 (MQ=255)                             cccGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTgg                                                                                                        >  2:480133/1‑140 (MQ=255)                             cccGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTgg                                                                                                        >  1:42749/1‑140 (MQ=255)                             cccGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTgg                                                                                                        >  1:586341/1‑140 (MQ=255)                               cGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGgcg                                                                                                      <  1:571008/140‑1 (MQ=255)                               cGGCAAACTCTACCTAGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGgcg                                                                                                      <  1:118266/140‑1 (MQ=255)                                  cAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCt                                                                                                    <  2:566258/139‑1 (MQ=255)                                  cAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCt                                                                                                    >  1:566258/1‑139 (MQ=255)                                  cAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGACCGTCTTCAAGGTCATGTATCAGCGGAACATCAGCACATTTGGCGCTg                                                                                                   >  2:236301/1‑140 (MQ=255)                                      ctctACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCtt                                                                                               <  2:42749/140‑1 (MQ=255)                                                tCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGaa                                                                                     <  2:515321/140‑1 (MQ=255)                                                tCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGaa                                                                                     <  1:403008/140‑1 (MQ=255)                                                tCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGaa                                                                                     >  2:491750/1‑140 (MQ=255)                                                           cGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAaaga                                                                          <  2:627164/140‑1 (MQ=255)                                                           cGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTAGCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAaaga                                                                          <  2:732724/140‑1 (MQ=255)                                                             gTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAaagaag                                                                        >  1:395060/1‑140 (MQ=255)                                                                ccGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGt                                                                     >  1:76244/1‑140 (MQ=255)                                                                     ccTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATc                                                                >  1:2101/1‑140 (MQ=255)                                                                             ctgctgGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGa                                                        <  2:2101/140‑1 (MQ=255)                                                                                ctgGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACcg                                                     >  1:526406/1‑140 (MQ=255)                                                                                      aaTTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGgcggcg                                               <  2:526406/140‑1 (MQ=255)                                                                                        tttCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGAc                                             <  1:48444/140‑1 (MQ=255)                                                                                                  cGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGa                                      >  2:64839/1‑137 (MQ=255)                                                                                                  cGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGa                                      <  1:64839/137‑1 (MQ=255)                                                                                                            ttATCACGATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAg                         <  1:293531/140‑1 (MQ=255)                                                                                                                   gATATTCTCGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGa                  <  1:480133/140‑1 (MQ=255)                                                                                                                         ctcGCCCGTCTTCCACGTCATACATCAGCCCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATcg            <  1:199186/140‑1 (MQ=255)                                                                                                                          tcGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGa                  >  2:509150/1‑133 (MQ=255)                                                                                                                          tcGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGa                  <  1:509150/133‑1 (MQ=255)                                                                                                                          tcGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATcgc           >  1:645352/1‑140 (MQ=255)                                                                                                                          tcGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCAGCGACTGACTGAACAGGTTACGGAGCGCCGGAAGATcgc           >  2:199186/1‑140 (MQ=255)                                                                                                                          tcGCCCGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAACGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATcgc           >  2:536071/1‑140 (MQ=255)                                                                                                                               cGTCTTCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTcc      <  2:586341/140‑1 (MQ=255)                                                                                                                                   ttCCAGGTCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTGCCCGAc  <  2:76244/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAC  >  NC_000913/1240391‑1240660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑