| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,302,129 | T→C | R316R (CGT→CGC) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,302,129 | 0 | T | C | 97.7% | 147.8 / NA | 44 | R316R (CGT→CGC) | oppA | oligopeptide ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (28/15); minor base G (1/0); total (29/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCATGCCGATCGAATTGTTCCAGAAGCTG‑AAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTG > NC_000913/1301998‑1302263 | aGCATGCCGATCGAATTGTTCCAGAAGCTG‑AAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACt < 2:150201/140‑1 (MQ=255) cATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCAcc < 2:473540/134‑1 (MQ=255) cATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCAcc > 1:473540/1‑134 (MQ=255) tGCCGATCGAATTGTTCCAGAAGCTG‑AAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCTACGCTGGGCGTGTACGCACCGCACTGaaa > 1:302218/1‑140 (MQ=255) gCCGATCGAATTGTTCCTGAAGCTG‑AAAAAAGAAATCCCCGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGTGTGTGCGCACCGCACTGAAAc < 2:343996/140‑1 (MQ=255) gCCGATCGAATTGTTCCGGAAGCTG‑AAAAAAGAGACCCCCGACGAAGTGCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAAc < 2:230650/140‑1 (MQ=255) aaTTGTTCCAGAAGCCG‑AAAAACGACATCCCGGACGATGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATg < 1:202897/140‑1 (MQ=255) aTTGTTCCAGAAGCTG‑AAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑TCGCCATTCAACGTTGTGCGTGTGCGGACCGAACTGAAACTAGGTATgg > 2:189430/1‑140 (MQ=255) tG‑AAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCAtt > 2:714675/1‑140 (MQ=255) tG‑AAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCAtt > 1:381254/1‑140 (MQ=255) aagaTCCCGGACGAAGTTCATGGTGTTCCATCCCTCTGCAGTTACTATTTCGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaataa < 2:229781/139‑1 (MQ=255) aaGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAg > 2:708919/1‑140 (MQ=255) aaGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAg < 1:714675/140‑1 (MQ=255) aTCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGccc > 2:180285/1‑140 (MQ=255) aTCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGccc > 2:687696/1‑140 (MQ=255) cATCCCTGTGCACTTACTATAAC‑AAAATAACAAACAAAAACCCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAACCTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGcc < 2:381254/140‑1 (MQ=255) cccTGTGCATTTACTATTACAAAATTAACACCCAGAAC‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAt < 2:580419/139‑1 (MQ=255) gtgCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt > 2:436758/1‑140 (MQ=255) tgCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa > 1:38831/1‑140 (MQ=255) tgCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa > 2:544310/1‑140 (MQ=255) tgCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa > 2:645461/1‑140 (MQ=255) tgCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa > 2:371688/1‑140 (MQ=255) tgCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa > 2:375023/1‑140 (MQ=255) tgCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa > 1:550516/1‑140 (MQ=255) tgCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa > 1:515084/1‑140 (MQ=255) gCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATTATTGTTAATAAACTAAAAGCGCAGGCCAACATGCCCGCCCATGGTTac > 2:337069/1‑140 (MQ=255) cACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTaca < 1:371688/140‑1 (MQ=255) ttACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTc > 1:739285/1‑140 (MQ=255) ttACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTc < 1:544310/140‑1 (MQ=255) ttACTATTACGAAATTAAC‑ACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTcc > 2:33467/1‑140 (MQ=255) aCTATTACGAAATTAACAACGAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa > 2:150420/1‑140 (MQ=255) aCTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTccc > 1:580419/1‑139 (MQ=255) aCTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa < 1:645461/140‑1 (MQ=255) aCTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa > 1:565602/1‑140 (MQ=255) aTTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCg > 1:525241/1‑140 (MQ=255) tACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGta > 1:82702/1‑140 (MQ=255) gAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAc > 1:713203/1‑140 (MQ=255) aaTTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTg > 1:125042/1‑140 (MQ=255) aaacacCAAAAACCCGCCATTCAACGATGGGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATg < 2:739285/136‑1 (MQ=255) aGAAA‑CCGCCATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCaaaa < 2:525241/140‑1 (MQ=255) ccATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCa > 1:710803/1‑140 (MQ=255) cATTCAACGATGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAg > 2:601167/1‑140 (MQ=255) aTGTGCGTGTGCGCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATgg > 2:612039/1‑140 (MQ=255) tgtgcgtgtgcgCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGt < 1:436758/140‑1 (MQ=255) tgtgcgtgtgcgCACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGt > 2:724614/1‑140 (MQ=255) tgcgtgtgcgcACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGttt > 2:2194/1‑140 (MQ=255) gtgcgcACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTg < 2:82702/140‑1 (MQ=255) | AGCATGCCGATCGAATTGTTCCAGAAGCTG‑AAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAA‑CCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTG > NC_000913/1301998‑1302263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |