Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,314,648	A→G	Y210C (TAT→TGT)	ompW →	outer membrane protein W

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,314,648	0	A	G	100.0%	211.2 / NA	59	Y210C (TAT→TGT)	ompW	outer membrane protein W
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/29);  total (30/29)

GTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGA  >  NC_000913/1314519‑1314781                                                                                                                                  |                                                                                                                                      gTGACTGGTTGGTTAACATGTCAGTGTGGCACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTaa                                                                                                                             >  1:485678/1‑140 (MQ=255)    aCTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCGCAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTc                                                                                                                          >  2:719514/1‑140 (MQ=255)    aCTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTc                                                                                                                          >  1:146772/1‑140 (MQ=255)    aCTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTc                                                                                                                          >  2:127192/1‑140 (MQ=255)    aCTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTCGGTGTTTATGTACTTAGCAGGCTGTCGTTTTTAATTc                                                                                                                          >  1:303579/1‑140 (MQ=255)                   tGTCAGTGTGGTACACGGATATCGACACCTCCGCCAAGTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGAcc                                                                                                           <  1:258124/140‑1 (MQ=255)                     tCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGAcccc                                                                                                         >  1:638435/1‑140 (MQ=255)                      cAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCg                                                                                                        <  1:501323/140‑1 (MQ=255)                         tgtgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTaa                                                                                                     >  2:393114/1‑140 (MQ=255)                              tACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATAc                                                                                                >  1:122713/1‑140 (MQ=255)                              tACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATAc                                                                                                >  1:137460/1‑140 (MQ=255)                              tACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCAAAAAAACGACCCCGTAATATAc                                                                                                >  2:675974/1‑140 (MQ=255)                                cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgg                                                                                              <  2:638435/140‑1 (MQ=255)                                cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGAACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGATTTTAATTCCGAACAAAAACGTCCCCGTAATATACgg                                                                                              >  1:354898/1‑140 (MQ=255)                                  tGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                            <  2:122713/140‑1 (MQ=255)                                  tGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                            <  1:127192/140‑1 (MQ=255)                                   ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGAcc                                                                                                           >  2:8615/1‑124 (MQ=255)                                   ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGAcc                                                                                                           <  1:8615/124‑1 (MQ=255)                                   ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                           >  2:69018/1‑140 (MQ=255)                                   ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                           >  2:688844/1‑140 (MQ=255)                                   ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                           >  1:2710/1‑140 (MQ=255)                                   ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                           >  2:511056/1‑140 (MQ=255)                                        tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTATTTCAGCAAAAAAACGAACCCGGAATATACGGGGTCAATa                                                                                      >  2:311743/1‑140 (MQ=255)                                        tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAAt                                                                                       <  2:363450/139‑1 (MQ=255)                                        tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAAt                                                                                       >  1:363450/1‑139 (MQ=255)                                           aTACCACCGCCAATTATAAGCTGGGCGGTGCACATGAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                   <  1:174886/140‑1 (MQ=255)                                           aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATa                                                                                      <  2:730437/137‑1 (MQ=255)                                           aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATa                                                                                      >  1:730437/1‑137 (MQ=255)                                            tACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGa                                                                                  <  1:69018/140‑1 (MQ=255)                                             accaccGCCAATTATCAGCTGGCCGCTGCTCAGCAACTCGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGAc                                                                                 <  1:201495/140‑1 (MQ=255)                                             accaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGAc                                                                                 <  1:688844/140‑1 (MQ=255)                                              ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACa                                                                                <  1:50516/140‑1 (MQ=255)                                              ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACa                                                                                <  2:137460/140‑1 (MQ=255)                                               caccGCCAATTATAATCTGGGCGGTACACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAt                                                                               <  1:311743/140‑1 (MQ=255)                                               caccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAt                                                                               >  2:517430/1‑140 (MQ=255)                                               caccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAt                                                                               <  2:2710/140‑1 (MQ=255)                                                accGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                                              <  1:511056/140‑1 (MQ=255)                                                 ccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                     >  1:728362/1‑132 (MQ=255)                                                 ccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                     <  2:728362/132‑1 (MQ=255)                                                    ccAATTATAAGCTGGGCGGTGCACAGCAGCACGGTAGCGTAGGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTAAATAAGGACATGGtat                                                                          <  2:485678/140‑1 (MQ=255)                                                        ttATAAGCTGGGCGGGGCACAGAAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                      <  1:293989/140‑1 (MQ=255)                                                              gCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatt                                                                >  2:387515/1‑140 (MQ=255)                                                                  ggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                      >  1:402710/1‑130 (MQ=255)                                                                  ggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                      <  2:402710/130‑1 (MQ=255)                                                                  ggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTAttt                                                            >  1:611299/1‑140 (MQ=255)                                                                   gCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATATGGGGTCAGTAACGACATGGTATAAAGGGTATTATttc                                                           >  1:218474/1‑140 (MQ=255)                                                                                 cacGATAGCGAACGCCGTGATCTGTGGGTGTATCTTTTCCCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGcc                                             <  2:354898/140‑1 (MQ=255)                                                                                   cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                           <  1:517430/140‑1 (MQ=255)                                                                                         cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGttt                                     >  1:724423/1‑140 (MQ=255)                                                                                           tACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa                                   >  1:420089/1‑140 (MQ=255)                                                                                                cTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCg                              >  1:438751/1‑140 (MQ=255)                                                                                                   gATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGcc                                             <  1:712335/122‑1 (MQ=255)                                                                                                   gATCCGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGcc                                             >  2:712335/1‑122 (MQ=255)                                                                                                       cGTGGGTGTTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCGCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaa                       <  2:146772/140‑1 (MQ=255)                                                                                                         tGGGTGTTTATGTTCTCTGCAGAATGTCGTTTTTAATTCTGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaaa                     <  2:303579/140‑1 (MQ=255)                                                                                                            gtgtTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAttt                  >  1:86490/1‑140 (MQ=255)                                                                                                              gtTTATGTTCTCAGCAGGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtct                <  1:547378/140‑1 (MQ=255)                                                                                                                          agcagGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTCCTACGCCACAAGGTTCAGAGCGAATAAAAAATTTCTCAGTTGTTTGCg    >  2:296388/1‑140 (MQ=255)                                                                                                                            cagGATGTCGTTTTTAATTCCGGACAAAAACCACCGCGTCATATACGGTGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGa  <  2:218474/140‑1 (MQ=255)                                                                                                                            cagGATGTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGa  <  1:719514/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      GTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGA  >  NC_000913/1314519‑1314781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑