Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,655	A→G	L325P (CTG→CCG)	sapD ←	putrescine ABC exporter ATP binding protein SapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,655	0	A	G	100.0%	109.5 / NA	32	L325P (CTG→CCG)	sapD	putrescine ABC exporter ATP binding protein SapD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

AGCGTAAAGCTCAAGGGTTTTACCGCTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCAGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGC  >  NC_000913/1352525‑1352790                                                                                                                                   |                                                                                                                                        aGCGTAAAGCTCAAGGGTTTTACCGCTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAAt                                                                                                                                >  2:375837/1‑140 (MQ=255)       aaGCTCAAGGGTTTTACCGCTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCTTCTCACTGTTTGTCAATGTTCGGCGGGAAATGACAgg                                                                                                                          >  1:226509/1‑140 (MQ=255)             aaGGGTTTTACCGCTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATaga                                                                                                                    >  1:494420/1‑140 (MQ=255)                   tttACCGCTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGAc                                                                                                                             <  2:727968/125‑1 (MQ=255)                   tttACCGCTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGAc                                                                                                                             >  1:727968/1‑125 (MQ=255)                    ttACCGCTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGAtt                                                                                                             >  1:31355/1‑140 (MQ=255)                          cTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGc                                                                                                       <  2:494420/140‑1 (MQ=255)                                     tGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAAc                                                                                            >  2:57203/1‑140 (MQ=255)                                       acgacgAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTATCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGt                                                                                          >  2:733145/1‑140 (MQ=255)                                         gacgaAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTTAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTgg                                                                                        <  1:500164/140‑1 (MQ=255)                                            gaAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGTTGAATTTTCGCCCCCGTCAAACGTGGCGt                                                                                     >  1:289609/1‑140 (MQ=255)                                             aaaCCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTc                                                                                    <  1:375837/140‑1 (MQ=255)                                                ccAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTcaca                                                                                 <  1:451102/140‑1 (MQ=255)                                                   gCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATg                                                                              >  2:375825/1‑140 (MQ=255)                                                      ggtccggtAGCGGAACGTTTTAATTAAATTACGCACTGCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCAt                                                                           <  1:300424/140‑1 (MQ=255)                                                       gtccggtAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGTTTTTTCGACCCGGTGAAACGTCGCGTCACAATGCAtt                                                                          >  1:223712/1‑140 (MQ=255)                                                          cggtAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTATCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATtctc                                                                       <  1:733145/140‑1 (MQ=255)                                                              aGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTtg                                                                   <  1:375825/140‑1 (MQ=255)                                                                  gAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCAtt                                                                          <  1:363531/129‑1 (MQ=255)                                                                  gAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCAtt                                                                          >  2:363531/1‑129 (MQ=255)                                                                  gAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCa                                                               >  2:559543/1‑140 (MQ=255)                                                                                            cAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGcccagac                                          >  1:533137/1‑131 (MQ=255)                                                                                            cAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGcccagac                                          <  2:533137/135‑5 (MQ=255)                                                                                             aaGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAAc                                   >  2:103777/1‑140 (MQ=255)                                                                                               gcagcGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCa                                 <  2:118606/140‑1 (MQ=255)                                                                                               gcagcGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCa                                 <  2:31355/140‑1 (MQ=255)                                                                                                      ttCGATCATCTCACTCTTTCTCCATGTTCGGTGGGGAATTACAGGCATGGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTa                          <  2:226509/140‑1 (MQ=255)                                                                                                              tctcACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTcc                  <  1:57203/140‑1 (MQ=255)                                                                                                                tcACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCcag                <  1:103777/140‑1 (MQ=255)                                                                                                                        tctcCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCTAGACGACCACCTATAGGTAACAGTTCCAGCAGTGGGa        >  1:337291/1‑140 (MQ=255)                                                                                                                        tctcCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCcagcag             >  1:639953/1‑135 (MQ=255)                                                                                                                        tctcCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCcagcag             <  2:639953/135‑1 (MQ=255)                                                                                                                              tGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAgcgc  >  2:138488/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        AGCGTAAAGCTCAAGGGTTTTACCGCTTCTACGGTCTGACGACGAAACCAGCCGGTCCGGTAGCGGAACGTTTTACTTAAATTACGCACTTCAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCAGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGC  >  NC_000913/1352525‑1352790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑