Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,426,089	T→C	intergenic (+109/‑365)	ydaY → / → ynaA	Rac prophage; putative uncharacterized protein YdaY/Rac prophage; putative prophage tail length tape measure domain‑containing protein YnaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,426,089	0	T	C	100.0%	106.6 / NA	32	intergenic (+109/‑365)	ydaY/ynaA	Rac prophage; putative uncharacterized protein YdaY/Rac prophage; putative prophage tail length tape measure domain‑containing protein YnaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

ATACGATAAAAAAGCCATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCC  >  NC_000913/1425961‑1426209                                                                                                                                 |                                                                                                                         aTACGATAAAAAAGCCATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAgt                                                                                                               <  1:370242/140‑1 (MQ=255) aTACGATAAAAAAGCCATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCAATACCGGTACCGGAAATAGACACGGCGCTTAAggctg                                                                                                               >  1:242533/1‑137 (MQ=255)       tAAAAAAGCCATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTtgttgt                                                                                                         >  2:682315/1‑140 (MQ=255)        aaaaaaGCCATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGG‑AAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAgttg                                                                                                             <  2:396750/134‑1 (MQ=255)        aaaaaaGCCATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGG‑AAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAgttg                                                                                                             >  1:396750/1‑134 (MQ=255)           aaaGCCATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGAAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGc                                                                                                     <  1:495282/140‑1 (MQ=255)            aaGCCATAGCTGCCTAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGcc                                                                                                    <  1:441091/140‑1 (MQ=255)                cATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTAtt                                                                                                >  1:71720/1‑140 (MQ=255)                cATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTAtt                                                                                                <  2:29847/140‑1 (MQ=255)                cATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTAtt                                                                                                <  1:729395/140‑1 (MQ=255)                  tAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCg                                                                                              <  1:696496/140‑1 (MQ=255)                  tAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCg                                                                                              >  2:165466/1‑140 (MQ=255)                             cGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCg                                                                                   >  1:680925/1‑140 (MQ=255)                                 ggtTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGcc                                                                               <  1:717925/140‑1 (MQ=255)                                  gtTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAGGGAGGTGTTGTACGCGTATTCGGTAATATCCCGTGccc                                                                              >  2:334648/1‑140 (MQ=255)                                  gtTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGccc                                                                              <  1:61961/140‑1 (MQ=255)                                    tATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGa                                                                            >  2:619863/1‑140 (MQ=255)                                    tATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGa                                                                            >  2:577747/1‑140 (MQ=255)                                           gTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATg                                                                     <  1:165466/140‑1 (MQ=255)                                                      aaaGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCt                                                          >  1:613319/1‑140 (MQ=255)                                                             ggAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGtgc                                                   >  1:458727/1‑140 (MQ=255)                                                               aaaaGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGtgcct                                                 <  2:504612/140‑1 (MQ=255)                                                                     cAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGtgcctttgcct                                           >  1:649751/1‑140 (MQ=255)                                                                      aaGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGtgcctttgcctt                                          <  1:545567/140‑1 (MQ=255)                                                                               gCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTAAGCTGGAAGGGCAGGGGTGCCTTTGCCTTTATCTCTGa                                 >  1:291183/1‑140 (MQ=255)                                                                                  agaAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACag                              >  1:401378/1‑140 (MQ=255)                                                                                             ggTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGaa                   >  2:379568/1‑140 (MQ=255)                                                                                              gTTGCCACCGATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGGGACTTTGCCTTTATCTCTGACAGAGATAAATGAat                  >  1:493342/1‑140 (MQ=255)                                                                                                       gATACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCa         <  1:682315/140‑1 (MQ=255)                                                                                                        aTACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCAc        <  1:42274/140‑1 (MQ=255)                                                                                                        aTACCGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCAc        <  2:688331/140‑1 (MQ=255)                                                                                                            cGGAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCAt    <  1:76420/140‑1 (MQ=255)                                                                                                              gAACCAGAAATAGACCCGGCGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATcc  <  2:71720/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                         ATACGATAAAAAAGCCATAGCTGCCCAGCGGTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCC  >  NC_000913/1425961‑1426209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑