Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,437,788	A→G	G999G (GGT→GGC)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,437,788	0	A	G	100.0%	140.4 / NA	40	G999G (GGT→GGC)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/24);  total (16/24)

CGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATCGTAAGCCCAGCCATCACCACCAATCAG  >  NC_000913/1437653‑1437910                                                                                                                                        |                                                                                                                           cgcgcCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGgccac                                                                                                                        <  2:732984/140‑1 (MQ=255)  gcgcCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGgccacc                                                                                                                       <  1:620458/140‑1 (MQ=255)  gcgcCGAGAGAAATCTGCGCCACATAAACATCACCCTACTTCATCATAGTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGgccacc                                                                                                                       <  2:191609/140‑1 (MQ=255)   cgcCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCg                                                                                                                      >  2:139211/1‑140 (MQ=255)     ccGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGgt                                                                                                                    <  2:21937/140‑1 (MQ=255)       gagagaAATCTGCGCTACATAACCACGACGGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGgtgt                                                                                                                  <  1:247307/140‑1 (MQ=255)       gagagaAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGgtgt                                                                                                                  <  1:139211/140‑1 (MQ=255)       gagagaAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGgtgt                                                                                                                  <  1:169338/140‑1 (MQ=255)        agagaAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTAGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGtt                                                                                                                 >  2:439339/1‑140 (MQ=255)                        caTAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTa                                                                                                 >  2:568508/1‑140 (MQ=255)                         aTAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTAt                                                                                                >  2:666557/1‑140 (MQ=255)                             aCATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAg                                                                                            <  2:417843/140‑1 (MQ=255)                                  aCCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCa                                                                                       <  1:572352/140‑1 (MQ=255)                                  aCCGTACATCATCATACCGACGCCAAGATCTGTACTCGCTTTACGTTTCCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCa                                                                                       <  2:231366/140‑1 (MQ=255)                                             tcatACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACg                                                                            >  1:433987/1‑140 (MQ=255)                                               atACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGtt                                                                          >  1:511372/1‑140 (MQ=255)                                                    gACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCg                                                                     <  2:480414/140‑1 (MQ=255)                                                     aCGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGt                                                                    >  2:461482/1‑140 (MQ=255)                                                     aCGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGt                                                                    >  2:682608/1‑140 (MQ=255)                                                     aCGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGt                                                                    >  1:22591/1‑140 (MQ=255)                                                      cGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACTGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTc                                                                   <  2:433987/140‑1 (MQ=255)                                                      cGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTc                                                                   >  2:168076/1‑140 (MQ=255)                                                      cGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTc                                                                   <  1:106096/140‑1 (MQ=255)                                                      cGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTc                                                                   <  1:523596/140‑1 (MQ=255)                                                      cGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTc                                                                   <  1:439339/140‑1 (MQ=255)                                                       gCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCa                                                                  <  2:388035/140‑1 (MQ=255)                                                        ccAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCaa                                                                 >  1:378936/1‑140 (MQ=255)                                                         cAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCaaa                                                                >  1:44557/1‑140 (MQ=255)                                                           aGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACt                                                              <  2:511372/140‑1 (MQ=255)                                                            gATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTc                                                             >  1:550920/1‑140 (MQ=255)                                                             aTCTTTACGCGCTTTACGTTTGTCGTGCTCGCCAAATTTGGTTACTGCGCCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCa                                                            <  2:238913/140‑1 (MQ=255)                                                             aTCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCa                                                            <  1:168076/140‑1 (MQ=255)                                                                 ttACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATAc                                                        <  2:1067/140‑1 (MQ=255)                                                                 ttACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATAc                                                        <  1:568508/140‑1 (MQ=255)                                                                  tACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACa                                                       <  2:633690/140‑1 (MQ=255)                                                                                ttGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGcc                                         <  2:22591/140‑1 (MQ=255)                                                                                        cTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGa                                 <  1:666557/140‑1 (MQ=255)                                                                                                aTTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATCGTaa                         >  2:93381/1‑140 (MQ=255)                                                                                                          tGCACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATCATAAGCCCAGCCAt               <  2:556786/140‑1 (MQ=255)                                                                                                            cACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTGAATACATGATCCAGACCGCCAAAGCTGATACCGTAAGCCCAGCCATca             >  2:305900/1‑140 (MQ=255)                                                                                                            cACCCAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATCGTAAGCCCAGCCATca             >  2:184515/1‑140 (MQ=255)                                                                                                              cccAGCGGTGTCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATCGTAAGCCCAGCCATcacc           <  2:374677/140‑1 (MQ=255)                                                                                                                       gtCGCTTTCGACGCCTGGCCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATCGTAAGCCCAGCCATCACCACCAATCAg  <  2:378936/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                           CGCGCCGAGAGAAATCTGCGCCACATAAACATGACCGTACATCATCATACTGACGCCAAGATCTTTACGCGCTTTACGTTTGCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATCGTAAGCCCAGCCATCACCACCAATCAG  >  NC_000913/1437653‑1437910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑