Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,495,485	A→G	E66E (GAA→GAG)	ydcJ →	DUF1338 domain‑containing protein YdcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,495,485	0	A	G	100.0%	127.4 / NA	39	E66E (GAA→GAG)	ydcJ	DUF1338 domain‑containing protein YdcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/20);  total (19/20)

ATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACA  >  NC_000913/1495348‑1495614                                                                                                                                          |                                                                                                                                  aTGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCg                                                                                                                                 >  2:574692/1‑140 (MQ=255)            agaagTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGa                                                                                                                      >  1:568497/1‑140 (MQ=255)              aagTTCCGCAATATTGCACGTTGCTGGAACTGGTAGCTGATGTGACTCAGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGAAGAGCTGGAACGACTGAATGTTAAGCGTCATGGGGCGAtt                                                                                                                    >  2:356788/1‑140 (MQ=255)                      cAATATGGCACGCTGATGGAACTGGCAGCTGATGTGAGTCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTgg                                                                                                            <  1:339445/140‑1 (MQ=255)                        atatGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGa                                                                                                          <  2:490292/140‑1 (MQ=255)                        atatGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGa                                                                                                          >  1:490292/1‑140 (MQ=255)                         tatGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGAc                                                                                                         >  1:117458/1‑140 (MQ=255)                              cACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGcac                                                                                                    <  2:117458/140‑1 (MQ=255)                                  ctgctgGAACTGGTAGCTGATGCGAATCTGGATGTACTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAaga                                                                                                <  2:277655/140‑1 (MQ=255)                                   tgctgGAACTGGTAGCTGTTGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAagag                                                                                               >  2:30017/1‑140 (MQ=255)                                             ggTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTActc                                                                                     >  1:524036/1‑140 (MQ=255)                                                   tGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTcggc                                                                               <  1:111707/140‑1 (MQ=255)                                                          aaTCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGGGATTCGCGCTGGAACTCCACAAGAGCTTGCTACTCTTCGGCGGATGtt                                                                        >  2:243130/1‑140 (MQ=255)                                                           aTCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGttt                                                                       <  1:381552/140‑1 (MQ=255)                                                             cTGGCTGTGCTGGAAGACAATCTTCAACTTCACGAAATAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGc                                                                     <  1:234557/140‑1 (MQ=255)                                                               ggCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCa                                                                   <  2:696503/140‑1 (MQ=255)                                                                    tgCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTa                                                                >  1:140941/1‑138 (MQ=255)                                                                    tgCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTa                                                                <  2:140941/138‑1 (MQ=255)                                                                      cTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATggg                                                            >  1:623632/1‑140 (MQ=255)                                                                       tGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATgggg                                                           <  1:574692/140‑1 (MQ=255)                                                                          aaaaCAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATg                                                        >  2:377911/1‑140 (MQ=255)                                                                              cAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCTGCGGATGTTTGCCTTTATGGGGATGTAcc                                                    >  1:267215/1‑140 (MQ=255)                                                                                 tCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCgg                                                 <  1:689232/140‑1 (MQ=255)                                                                                     cAACTGCACGAAAAAATTGTAATTGCAGACGTGCTGGCGGGACTGAATAATGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAg                                             <  2:248317/140‑1 (MQ=255)                                                                                     cAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGTGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAg                                             <  1:356788/140‑1 (MQ=255)                                                                                        cTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTa                                          >  1:484048/1‑140 (MQ=255)                                                                                           cACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGCTGTTTGCAATTTTGAGGATGTTCCCGGTGAGCTATTa                                       >  1:356298/1‑140 (MQ=255)                                                                                           cACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTa                                       >  2:388481/1‑140 (MQ=255)                                                                                           cACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTa                                       >  2:670727/1‑140 (MQ=255)                                                                                           cACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGATGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTAttg                                       >  2:717812/1‑139 (MQ=255)                                                                                                     tGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCg                             >  1:267060/1‑140 (MQ=255)                                                                                                             gCAGACGAGCTGGCGCGGCTGAGTGTTGAGCGTCAGGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGcaggcagg                     <  2:267215/140‑1 (MQ=255)                                                                                                             gCAGACGAACTGGCGCAACTGAATGTTGAGTATCACGGGGCGATTCGCATTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGcaggcagg                     <  2:356298/140‑1 (MQ=255)                                                                                                               aGACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAgggg                   <  2:675030/140‑1 (MQ=255)                                                                                                                gACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtctctc                              >  1:591427/1‑128 (MQ=255)                                                                                                                gACGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtctctc                              <  2:591427/128‑1 (MQ=255)                                                                                                                 aCGAGCTGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTg                 <  1:30017/140‑1 (MQ=255)                                                                                                                       tGGCGCGACTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTa           >  2:123393/1‑140 (MQ=255)                                                                                                                               cTGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTGCTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGAc   <  1:536950/140‑1 (MQ=255)                                                                                                                                tGAATGTTGAGCGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACa  <  2:623632/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  ATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGAAAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACA  >  NC_000913/1495348‑1495614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑